Hepatocyte Transplantation
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Hepatocyte Transplantation
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Author : S. Gupta
language : en
Publisher: Springer Science & Business Media
Release Date : 2002-09-30
Hepatocyte Transplantation written by S. Gupta and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-09-30 with Medical categories.
In recent years there has been an increasing need for transplantation, but the number of donor livers available has increased only slightly, despite intensive public relations activities. New concepts in the field of transplantation, for instance the transplantation of living donor organs or the splitting of organs, are urgently required, to safeguard the treatment of patients with severe liver disease. The development and clinical application of cell therapy for patients with liver disease could soon present a significant enhancement of the therapeutic options. The aim of such cell therapy is to repair or improve the biological function of the chronically and acutely damaged liver. Even though systematic trials are not available, individual case reports and small series already show promising clinical results. Present concepts of cell therapy for liver diseases based on the use of primary hepatocytes have recently been considerably extended through new data on the biology of stem cells. The adult haematopoetic stem cell as a pool for hepatocyte grafts - what would be the perspectives for the clinical application? This book is the proceedings of the Falk Symposium No. 126 on `Hepatocyte Transplantation' (Progress in Gastroenterology and Hepatology Part III) held in Hannover, Germany, October 2-3, 2001, and is a forum for basic research, but also for questions concerning clinical applications in the field of hepatocyte transplantation.
Hepatocyte Transplantation
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Author : Anil Dhawan
language : en
Publisher: Humana Press
Release Date : 2010-07-19
Hepatocyte Transplantation written by Anil Dhawan and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-07-19 with Science categories.
Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease.
Cell Labelling For Hepatocyte Transplantation
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Author : Juliana Puppi
language : en
Publisher:
Release Date : 2011
Cell Labelling For Hepatocyte Transplantation written by Juliana Puppi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with Liver cells categories.
Hepatocyte transplantation is being evaluated as an alternative to orthotopic liver transplantation in the management of liver-based metabolic disorders and acute liver failure. The use of magnetic resonance imaging (MRI) as a non-invasive technique to monitor donor cell engraftment following transplantation was investigated. -- Studies were performed to optimise labelling conditions for human hepatocytes with clinically available superparamagnetic iron oxide nanoparticles (SPIOs). Labelling with 50 g Fe/ml of SPIOs in culture had minimal effect on cell metabolic function and allowed label incorporation at sufficient levels for in vitro detection on a 7-T MRI system. Intrasplenic transplantation of human hepatocytes labelled with SPIOs was performed in non-obese diabetic/severe combined immunodeficient (NOD-SCID) mice. Two hours after hepatocyte transplantation, SPIO-labelled hepatocytes could be imaged in the liver. -- Incorporation of the contrast agent in hepatocytes was significantly improved using the transfection agents poly-L-lysine and protamine sulphate (PS). A rat model of acute liver failure induced by D(+)-galactosamine was used to study the engraftment potential of the labelled cells in vivo. At day 7 there was a decrease in the MRI signal intensity in the liver of rats transplanted with SPIO-PS complex-labelled cells. However, histology demonstrated that the iron in the liver was in Kupffer and endothelial cells in periportal areas rather than hepatocytes, indicating clearance of labelled hepatocytes. Donor cells were detected in the spleen of animals receiving SPIO-PS labelled hepatocytes, suggesting that some of the cells were retained in the spleen in this model. -- As an alternative approach, 99mTechnetium-labelled galactosyl-serum albumin, which binds to asialoglycoprotein receptors on the surface of hepatocytes was also investigated.
Hepatocyte Transplantation
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Author : Mike Morris
language : en
Publisher: Createspace Independent Publishing Platform
Release Date : 2017-12-14
Hepatocyte Transplantation written by Mike Morris and has been published by Createspace Independent Publishing Platform this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-14 with categories.
Hepatocyte Transplantation guides readers through laboratory protocols for the generation of humanized livers for the assessment of biological actions in vivo and techniques to monitor cell engraftment after cell transplantation in vivo are described and procedures for computational analyses of hepatocyte transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Pediatric Liver Transplantation
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Author : Nedim Hadzic
language : en
Publisher: Elsevier Health Sciences
Release Date : 2020-11-13
Pediatric Liver Transplantation written by Nedim Hadzic and has been published by Elsevier Health Sciences this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-11-13 with Medical categories.
Written and edited by global leaders in the field, Pediatric Liver Transplantation: A Clinical Guide covers all aspects of treatment and management regarding this multifaceted procedure and unique patient population. This practical reference offers detailed, focused guidance in a highly templated, easy-to-consult format, covering everything from pre-transplantation preparation to surgical techniques to post-operative complications. Provides an in-depth understanding of all aspects of pediatric liver transplantation, ideal for pediatric hepatologists, pediatric transplant surgeons, and others on the pediatric transplant team. Covers all surgical techniques in detail, including split graft, living related, auxiliary, and domino. Discusses pediatric liver transplantation consideration for an increasing number of additional metabolic, hematologic and renal conditions; breakthroughs in grafting and stem cell therapy; and techniques and present role of hepatocyte transplantation. Uses a quick-reference templated format; each chapter includes an overview, pathophysiology, conventional management, controversies, and bulleted summary of key take-aways. Includes state-of-the-art mini-reviews based on updated references and author experience throughout the text. Features a full-color design with numerous algorithms, figures, and radiological and histopathological photos.
Understanding And Manipulating Transplanted Hepatocyte Death
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Author : Molly Kay Smith
language : en
Publisher:
Release Date : 2004
Understanding And Manipulating Transplanted Hepatocyte Death written by Molly Kay Smith and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with categories.
The Hepatocyte Review
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Author : M. N. Berry
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
The Hepatocyte Review written by M. N. Berry and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.
It is thirty years since the technique of high-yield preparation of isolated hepatocytes, by collagenase perfusion of the liver, was published. The original method described by Berry and Friend has undergone many minor modifications by other workers, and the two-step procedure introduced by Seglen in 1976 has become the most frequent way to prepare hepatocyte suspensions. An important development introduced by Bissell in 1973 was the use of the cells as the first step in monolayer culture. The availability of the isolated hepatocyte preparation as cells in suspension or culture has undoubtedly facilitated research on the liver. This was emphasised in our book, published (with Dr. Greg Barritt) in 1990, which described in detail methods of preparation and the properties of the isolated hepatocytes. It also discussed the usefulness of the preparation for the study of intermediary and xenobiotic metabolism, calcium ion transport, and the growth and differentiation of hepatocytes in culture. The book also touched briefly on a range of specialised techniques, including peri fusion, subcellular fractionation, transplantation, cryopreservation and measurement of intracellular pH. Although standard procedures for the manipulation of hepatocytes have not changed a great deal in ten years, they have undoubtedly been refined. This applies particularly to hepatocyte culture techniques, cryopreservation, and even to preparation of hepatocyte suspensions, where it is now feasible to use purified enzymes. There is also much more emphasis on the use and study of human hepatocytes, particularly in the field of pharmacology and therapeutics.
Hepatocyte Transplantation
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Author : Joseph Pieter Anna Maria Vroemen
language : en
Publisher:
Release Date : 1987
Hepatocyte Transplantation written by Joseph Pieter Anna Maria Vroemen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with categories.
Hepatocyte Transplantation
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Author : Anil Dhawan
language : en
Publisher: Humana Press
Release Date : 2009-01-28
Hepatocyte Transplantation written by Anil Dhawan and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-28 with Science categories.
Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease.
Hepatocyte Transplantation
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Author :
language : en
Publisher:
Release Date : 2015
Hepatocyte Transplantation written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.