Cell Labelling For Hepatocyte Transplantation
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Cell Labelling For Hepatocyte Transplantation
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Author : Juliana Puppi
language : en
Publisher:
Release Date : 2011
Cell Labelling For Hepatocyte Transplantation written by Juliana Puppi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with Liver cells categories.
Hepatocyte transplantation is being evaluated as an alternative to orthotopic liver transplantation in the management of liver-based metabolic disorders and acute liver failure. The use of magnetic resonance imaging (MRI) as a non-invasive technique to monitor donor cell engraftment following transplantation was investigated. -- Studies were performed to optimise labelling conditions for human hepatocytes with clinically available superparamagnetic iron oxide nanoparticles (SPIOs). Labelling with 50 g Fe/ml of SPIOs in culture had minimal effect on cell metabolic function and allowed label incorporation at sufficient levels for in vitro detection on a 7-T MRI system. Intrasplenic transplantation of human hepatocytes labelled with SPIOs was performed in non-obese diabetic/severe combined immunodeficient (NOD-SCID) mice. Two hours after hepatocyte transplantation, SPIO-labelled hepatocytes could be imaged in the liver. -- Incorporation of the contrast agent in hepatocytes was significantly improved using the transfection agents poly-L-lysine and protamine sulphate (PS). A rat model of acute liver failure induced by D(+)-galactosamine was used to study the engraftment potential of the labelled cells in vivo. At day 7 there was a decrease in the MRI signal intensity in the liver of rats transplanted with SPIO-PS complex-labelled cells. However, histology demonstrated that the iron in the liver was in Kupffer and endothelial cells in periportal areas rather than hepatocytes, indicating clearance of labelled hepatocytes. Donor cells were detected in the spleen of animals receiving SPIO-PS labelled hepatocytes, suggesting that some of the cells were retained in the spleen in this model. -- As an alternative approach, 99mTechnetium-labelled galactosyl-serum albumin, which binds to asialoglycoprotein receptors on the surface of hepatocytes was also investigated.
Hepatocyte Transplantation
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Author : S. Gupta
language : en
Publisher: Springer Science & Business Media
Release Date : 2002-09-30
Hepatocyte Transplantation written by S. Gupta and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-09-30 with Medical categories.
In recent years there has been an increasing need for transplantation, but the number of donor livers available has increased only slightly, despite intensive public relations activities. New concepts in the field of transplantation, for instance the transplantation of living donor organs or the splitting of organs, are urgently required, to safeguard the treatment of patients with severe liver disease. The development and clinical application of cell therapy for patients with liver disease could soon present a significant enhancement of the therapeutic options. The aim of such cell therapy is to repair or improve the biological function of the chronically and acutely damaged liver. Even though systematic trials are not available, individual case reports and small series already show promising clinical results. Present concepts of cell therapy for liver diseases based on the use of primary hepatocytes have recently been considerably extended through new data on the biology of stem cells. The adult haematopoetic stem cell as a pool for hepatocyte grafts - what would be the perspectives for the clinical application? This book is the proceedings of the Falk Symposium No. 126 on `Hepatocyte Transplantation' (Progress in Gastroenterology and Hepatology Part III) held in Hannover, Germany, October 2-3, 2001, and is a forum for basic research, but also for questions concerning clinical applications in the field of hepatocyte transplantation.
Hepatocyte Transplantation
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Author : Anil Dhawan
language : en
Publisher: Humana Press
Release Date : 2010-07-19
Hepatocyte Transplantation written by Anil Dhawan and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-07-19 with Science categories.
Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease.
Ex Vivo Cell Therapy
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Author : Klaus Schindhelm
language : en
Publisher: Academic Press
Release Date : 1999
Ex Vivo Cell Therapy written by Klaus Schindhelm and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Health & Fitness categories.
R.E. Nordon and K. Schindhelm, Introduction. -- L. Robb, A.G. Elefanty, and C.G. Begley, Transcriptional Control of Hematopoieses. -- R. Starr and N.A. Nicola, Cell Signaling by Hemopoietic Growth Factor Receptors. -- P.J. Simmons, D.N. Haylock, and J.-P. Lévesque, Influence of Cytokines and Adhesion Molecules on Hematopoietic Stem Cell Development. -- P.A. Rowlings, Allogeneic Hematopoietic Stem Cell Transplantation. -- U. Hahn and L.B. To, Autologous Stem Cell Transplantation. -- M.R. Vowels, Cord Blood Stem Cell Transplantation. -- S.R. Riddell, E.H. Warren, D. Lewinsohn, C. Yee, and P.D. Greenberg, Reconstitution of Immunity by Adoptive Immunotherapy with T Cells. -- L.Q. Sun, M. Miller, and G. Symonds, Exogenous Gene Transfer into Lymphoid and Hematopoietic Progenitor Cells. -- C. Dowding, T. Leemhuis, A. Jakubowski, and C. Reading, Process Development for Ex Vivo Cell Therapy. -- R.E. Nordon and K. Schindhelm, Cell Separation. -- P.W. Zandstra, C.J. Eaves, and J.M. Piret, Environ ...
Hepatocyte Transplantation
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Author : Michio Mito
language : en
Publisher: S. Karger AG (Switzerland)
Release Date : 1997
Hepatocyte Transplantation written by Michio Mito and has been published by S. Karger AG (Switzerland) this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Medical categories.
The rapidly increasing frequency of organ transplantation is causing a severe shortage of donor organs. Hepatocyte transplantation has thus been studied to develop an alternative therapeutic method for patients with various liver diseases. In experimental studies using rodents, transplanted hepatocytes survive and function in ectopic sites for a long period of time. Recent advances in molecular biology and tissue engineering enable us to manipulate isolated hepatocytes before transplantation. Before clinical application of this new therapeutic method, there are many issues to be resolved such as determining an optimal location for transplantation, rapid proliferation of transplanted hepatocytes, transplantation in large animals, immunological reactions and long-term gene expression. In this book, authorities on hepatocyte transplantation from all over the world describe progress in this field and discuss the unsolved problems. For researchers and clinicians interested in the liver, this book will be the definitive resource on hepatocyte transplantation.
Hepatocyte Transplantation
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Author : Anil Dhawan
language : en
Publisher: Humana Press
Release Date : 2009-01-28
Hepatocyte Transplantation written by Anil Dhawan and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-28 with Science categories.
Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease.
Stem Cell Labeling For Delivery And Tracking Using Noninvasive Imaging
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Author : Dara L. Kraitchman
language : en
Publisher: CRC Press
Release Date : 2011-11-18
Stem Cell Labeling For Delivery And Tracking Using Noninvasive Imaging written by Dara L. Kraitchman and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-11-18 with Science categories.
Stem Cell Labeling for Delivery and Tracking Using Noninvasive Imaging provides a comprehensive overview of cell therapy imaging, ranging from the basic biology of cell therapeutic choices to the preclinical and clinical applications of cell therapy. It emphasizes the use of medical imaging for therapeutic delivery/targeting, cell tracking, and determining therapeutic efficacy. The book first presents background information and insight on the major classes of stem and progenitor cells. It then describes the main imaging modalities and state-of-the-art techniques that are currently employed for stem cell tracking. In the final chapters, leading scholars offer clinical perspectives on existing and potential uses of stem cells as well as the impact of image-guided delivery and tracking in major organ systems. Through clear descriptions and color images, this volume illustrates how noninvasive imaging is used to track stem cells as they repair damaged tissue in the body. With contributions from some of the most prominent preclinical and clinical researchers in the field, the book helps readers to understand the evolving concepts of stem cell labeling and tracking as the field continues to move forward.
Liver Stem Cells
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Author : Takahiro Ochiya
language : en
Publisher: Humana Press
Release Date : 2011-12-14
Liver Stem Cells written by Takahiro Ochiya and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-12-14 with Science categories.
Increasing evidence suggests that liver stem cells have the capacity to differentiate into parenchymal hepatocytes or into bile ductular cells. These stem cells may be activated to proliferate after severe liver injury or exposure to hepatocarcinogens. Stem cell replacement strategies are being investigated as an alternative approach to liver repair and regeneration. Additionally, stem cell transplantation has been shown to significantly improve liver function and increase survival in experimentally-induced liver-injury models in animals. In Liver Stem Cells: Methods and Protocols, expert researchers focus on several hepatic progenitor cells, hepatic differentiation form stem cells, bile ductal cell formation from stem cells, liver stem cells and hepatocarcinogenesi, and application of liver stem cells for cell therapy. These topics shed light on stem cell technology which may lead to the development of effective clinical modalities for human liver diseases. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive, Liver Stem Cells: Methods and Protocols seeks to aid scientists in the further study of preclinical and clinical investigations that explore the therapeutic potential of stem cells in repair of liver injuries.
Stem Cells And The Future Of Regenerative Medicine
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Author : Institute of Medicine
language : en
Publisher: National Academies Press
Release Date : 2002-01-25
Stem Cells And The Future Of Regenerative Medicine written by Institute of Medicine and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-01-25 with Science categories.
Recent scientific breakthroughs, celebrity patient advocates, and conflicting religious beliefs have come together to bring the state of stem cell researchâ€"specifically embryonic stem cell researchâ€"into the political crosshairs. President Bush's watershed policy statement allows federal funding for embryonic stem cell research but only on a limited number of stem cell lines. Millions of Americans could be affected by the continuing political debate among policymakers and the public. Stem Cells and the Future of Regenerative Medicine provides a deeper exploration of the biological, ethical, and funding questions prompted by the therapeutic potential of undifferentiated human cells. In terms accessible to lay readers, the book summarizes what we know about adult and embryonic stem cells and discusses how to go about the transition from mouse studies to research that has therapeutic implications for people. Perhaps most important, Stem Cells and the Future of Regenerative Medicine also provides an overview of the moral and ethical problems that arise from the use of embryonic stem cells. This timely book compares the impact of public and private research funding and discusses approaches to appropriate research oversight. Based on the insights of leading scientists, ethicists, and other authorities, the book offers authoritative recommendations regarding the use of existing stem cell lines versus new lines in research, the important role of the federal government in this field of research, and other fundamental issues.
Cellular Transplantation
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Author : Craig Halberstadt
language : en
Publisher: Elsevier
Release Date : 2011-10-10
Cellular Transplantation written by Craig Halberstadt and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-10 with Science categories.
There have been tremendous strides in cellular transplantation in recent years, leading to accepted practice for the treatment of certain diseases, and use for many others in trial phases. The long history of cellular transplantation, or the transfer of cells from one organism or region of the body to another, has been revolutionized by advances in stem cell research, as well as developments in gene therapy. Cellular Transplants: From Lab to Clinic provides a thorough foundation of the basic science underpinning this exciting field, expert overviews of the state-of-the-art, and detailed description of clinical success stories to date, as well as insights into the road ahead. As highlighted by this timely and authoritative survey, scale-up technologies and whole organ transplantation are among the hurdles representing the next frontier. The contents are organized into four main sections, with the first covering basic biology, including transplant immunology, the use of immunosuppressive drugs, stem cell biology, and the development of donor animals for transplantation. The next part looks at peripheral and reconstructive applications, followed by a section devoted to transplantation for diseases of the central nervous system. The last part presents efforts to address the key challenges ahead, such as identifying novel transplantable cells and integrating biomaterials and nanotechnology with cell matrices. Provides detailed description of clinical trials in cell transplantation Review of current therapeutic approaches Coverage of the broad range of diseases addressed by cell therapeutics Discussion of stem cell biology and its role in transplantation