Human Amniotic Epithelial Cells
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Human Amniotic Epithelial Cells
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Author : Ruth Gomez Dominguez
language : en
Publisher:
Release Date : 2008
Human Amniotic Epithelial Cells written by Ruth Gomez Dominguez and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with categories.
Stem Cell Properties Of Human Amniotic Epithelial Cells
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Author : Sivakami Ilancheran
language : en
Publisher:
Release Date : 2007
Stem Cell Properties Of Human Amniotic Epithelial Cells written by Sivakami Ilancheran and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Amnion categories.
Amniotic Membrane
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Author : Ana Catarina Mamede
language : en
Publisher: Springer
Release Date : 2015-07-14
Amniotic Membrane written by Ana Catarina Mamede and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-07-14 with Medical categories.
This book describes the human amniotic membrane from its origin, characterization and medical applications, summarizing all the latest developments and findings related to this tissue with contributions from some of the leading researchers in the field. The book addresses issues for its preservation, separation and identification of amniotic membrane-derived cells, as well as the potential ethical issues involved in their use. The topic of this book is particularly pertinent for clinicians and researchers involved in the research and use of this tissue.
An Evaluation Of The Therapeutic Potential Of Human Amniotic Epithelial Cells During Ex Vivo Donor Lung Perfusion
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Author : Chelsea Paige Griffiths
language : en
Publisher:
Release Date : 2021
An Evaluation Of The Therapeutic Potential Of Human Amniotic Epithelial Cells During Ex Vivo Donor Lung Perfusion written by Chelsea Paige Griffiths and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021 with categories.
Regulation Of Cell Cycle During Maturation Of Human Amniotic Epithelial Cells Directed To Express Germ Cells And Primary Occyte Markers
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Author : Rawan Damouni Kampania
language : en
Publisher:
Release Date : 2016
Regulation Of Cell Cycle During Maturation Of Human Amniotic Epithelial Cells Directed To Express Germ Cells And Primary Occyte Markers written by Rawan Damouni Kampania and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with categories.
Human Amniotic Epithelial Cells Inhibit Cd4 T Cell Activation In Acute Kidney Injury Patients By Influencing The Mir 101 C Rel Il 2 Pathway
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Author :
language : en
Publisher:
Release Date : 2017
Human Amniotic Epithelial Cells Inhibit Cd4 T Cell Activation In Acute Kidney Injury Patients By Influencing The Mir 101 C Rel Il 2 Pathway written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.
Evaluating The Immunomodulatory Potential Of Human Amniotic Epithelial Cells As A Therapeutic In Ex Vivo Lung Reconditioning
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Author :
language : en
Publisher:
Release Date : 2017
Evaluating The Immunomodulatory Potential Of Human Amniotic Epithelial Cells As A Therapeutic In Ex Vivo Lung Reconditioning written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.
Introduction: Ex Vivo Lung Perfusion (EVLP) provides a platform for the evaluation and reconditioning of donor lungs deemed unsuitable for transplant. Elevated expression of pro-inflammatory cytokines, interleukin-8 (IL-8) and Tumour necrosis factor-u03b1 (TNFu03b1), in donor lungs are associated with worsened outcomes after transplantation. EVLP offers a platform to administer advanced therapeutics, including cell-based therapies such as human Amniotic Epithelial Cells (hAECs). This study aims to evaluate the immunomodulatory properties of hAECs against pro-inflammatory macrophage activation.Methods: hAECs were isolated from term-placenta through enzymatic digest and cultured for 3, 4 and 5 days to generate conditioned media. Isolated cells were characterised using immunocytochemistry and flow cytometry for surface markers. The THP-1 monocytic cell line was differentiated into macrophages using phorbol 12-myristate 13-acetate (PMA) then treated with IFNu03b3 and LPS to generate pro-inflammatory macrophages. ELISA and qPCR were used to detect any decrease in IL-8 and TNFu03b1 expression upon subsequent treatment with hAECs or their conditioned media.Results: Three term-placenta were used to harvest hAECs through a serum-free process, generating large yields of viable cells. Isolated hAECs strongly expressed epithelial cell markers Cytokeratin 19 and EpCAM (>90%) but not markers of endothelial or mesenchymal cells (u2264 2%). The THP-1 cell line was successfully polarised to pro-inflammatory macrophages, demonstrated by significantly increased protein expression of IL-8 and TNFu03b1. A significant decrease was observed for IL-8 and TNFu03b1 protein expression upon hAEC treatment of the pro-inflammatory macrophages at 6 and 24 hours, compared to the LPS control. hAEC treatment also lead to a 1.2 fold decrease in IL-8 gene expression compared to the LPS treated control at 6 hours.Discussion: In this study, we demonstrated the potential for hAECs to reduce the production of key inflammatory cytokines (IL-8 and TNFu03b1) from pro-inflammatory macrophages. Future studies plan to assess the therapeutic potential of hAECs within the EVLP platform.
Ex Vivo Expansion Of Human Limbal Epithelial Cells On Intact Human Amniotic Membrane
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Author : Elias Eduardo Hernandez Galindo
language : en
Publisher:
Release Date : 2004
Ex Vivo Expansion Of Human Limbal Epithelial Cells On Intact Human Amniotic Membrane written by Elias Eduardo Hernandez Galindo and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with categories.
Human Amniotic Membrane Basic Science And Clinical Application
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Author : Abdul Aziz Nather
language : en
Publisher: World Scientific
Release Date : 2017-08-23
Human Amniotic Membrane Basic Science And Clinical Application written by Abdul Aziz Nather and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-08-23 with Science categories.
This book is a comprehensive guide for all tissue bank operators to screen, procure and process amniotic membrane for clinical application.The amnion comes close to being the ideal biological membrane or dressing — readily available, inexpensive to procure and process. Its basic science is discussed in detail — anatomy, biological and biomechanical properties.It can be procured from the placenta in normal vaginal deliveries and from Caesarean Sections. Processing is by freeze-drying or by air-drying process with sterilisation using gamma irradiation.The product has low antigenicity, has anti-microbial properties with ability to enhance epithelisation with marked relief of pain. It is useful as a dressing for wounds — flap wounds, burn wounds, injury wounds, diabetic ulcers, leprous ulcers and post-surgery wounds and post-radiation wounds. It is also used as a biological scaffold for cells in tissue engineering. Its ophthalmic applications include treatment of corneal ulcers and conjunctival tumours. Oral uses include gingiva depigmentation and periodontal regeneration.
P005 Evaluating The Immunomodulatory Potential Of Human Amniotic Epithelial Cells As A Therapeutic In Ex Vivou00a0donor Lung Reconditioning
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Author :
language : en
Publisher:
Release Date : 2017
P005 Evaluating The Immunomodulatory Potential Of Human Amniotic Epithelial Cells As A Therapeutic In Ex Vivou00a0donor Lung Reconditioning written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.
Introduction: Ex Vivo Lung Perfusion (EVLP) provides a platform for the evaluation and reconditioning of donor lungs deemed unsuitable for transplant. Elevated expression of pro-inflammatory cytokines, interleukin-8 (IL-8) and Tumour necrosis factor-u03b1 (TNFu03b1), in donor lungs are associated with worsened outcomes after transplantation. EVLP offers a platform to administer advanced therapeutics, including cell-based therapies such as human Amniotic Epithelial Cells (hAECs). This study aims to evaluate the immunomodulatory properties of hAECs against pro-inflammatory macrophage activation.Methods: hAECs were isolated from term-placenta through enzymatic digest and cultured for 3, 4 and 5 days to generate conditioned media. Isolated cells were characterised using immunocytochemistry and flow cytometry for surface markers. The THP-1 monocytic cell line was differentiated into macrophages using phorbol 12-myristate 13-acetate (PMA) then treated with IFNu03b3 and LPS to generate pro-inflammatory macrophages. ELISA and qPCR were used to detect any decrease in IL-8 and TNFu03b1 expression upon subsequent treatment with hAECs or their conditioned media.Results: Three term-placenta were used to harvest hAECs through a serum-free process, generating large yields of viable cells. Isolated hAECs strongly expressed epithelial cell markers Cytokeratin 19 and EpCAM (>90%) but not markers of endothelial or mesenchymal cells (u2264 2%). The THP-1 cell line was successfully polarised to pro-inflammatory macrophages, demonstrated by significantly increased protein expression of IL-8 and TNFu03b1. A significant decrease was observed for IL-8 and TNFu03b1 protein expression upon hAEC treatment of the pro-inflammatory macrophages at 6 and 24 hours, compared to the LPS control. hAEC treatment also lead to a 1.2 fold decrease in IL-8 gene expression compared to the LPS treated control at 6 hours.Discussion: In this study, we demonstrated the potential for hAECs to reduce the production of key inflammatory cytokines (IL-8 and TNFu03b1) from pro-inflammatory macrophages. Future studies plan to assess the therapeutic potential of hAECs within the EVLP platform.