Human Embryonic Stem Cell Protocols
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Human Embryonic Stem Cell Protocols
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Author : KURSAD TURKSEN
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Human Embryonic Stem Cell Protocols written by KURSAD TURKSEN and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Embryonic Stem Cell Protocols
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Author : Kursad Turksen
language : en
Publisher: Springer Nature
Release Date : 2022-07-25
Embryonic Stem Cell Protocols written by Kursad Turksen and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-07-25 with Science categories.
This fully updated volume explores improved and extended protocols for embryonic stem cell (ESC) research, provided with the most direct and informative methodologies currently available. The book examines how these models for cell lineage and differentiation studies have continued to mature into a critical research workhorse. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Embryonic Stem Cell Protocols, Fourth Edition serves as an ideal resource for researching mining the depths of the ESC field.
Human Embryonic Stem Cell Protocols
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Author : ANONIMO
language : en
Publisher: Springer
Release Date : 2010-10-28
Human Embryonic Stem Cell Protocols written by ANONIMO and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-10-28 with Medical categories.
A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful Methods in Molecular Biologya series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Human Embryonic Stem Cells
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Author : Stephen Sullivan
language : en
Publisher: John Wiley & Sons
Release Date : 2007-06-13
Human Embryonic Stem Cells written by Stephen Sullivan and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-06-13 with Science categories.
With this valuable practical guide, three members of the Harvard Stem Cell Institute have compiled and edited the definite handbook for the exciting new field of human embryonic stem cell research. The editors have gathered protocols from scientists with extensive reputation and expertise, describing and comparing currently used techniques for the culture of human stem cells and discussing the strengths and weaknesses of the different approaches. Human Embryonic Stem Cells: The Practical Handbook contains the first centralised collection of methods used in human embryonic stem cell biology. The book covers the derivation of human stem cell lines, the obtaining of cells from human stem cell banks, the culturing and characterisation of the cells, and the differentiation of the cells in vitro and in vivo. Lastly, almost all of these protocols can also be used for analyzing and manipulating induced pluripotency iPS stem cells. This allows an even greater number of opportunities for those interested in purusing work in pluripotent stem cells, disease modelling, and other aspects of basic regenerative medicine research. The novel and useful focus of this book sets it apart from other available books: Compares and evaluates the protocols used in leading laboratories working on human embryonic stem cells Centred solely on practical protocols for human (not mouse) embryonic stem cell research Includes extensive troubleshooting sections Addresses the different proclivities and behaviours of individual human embryonic cell lines Contains techniques currently known only to a small number of specialised laboratories worldwide This handbook represents an essential source of up-to-date practical information for all cell and developmental biologists working with human embryonic stem cells or wishing to enter the field. It is also essential reading for clinical researchers in areas such as diabetes, cardiovascular disease, and neurological diseases. Praise from the reviews: "...a highly readable and useful book... A notable feature of the book is its air of openness and honesty... This book... will help many to navigate the uncharted waters of human embryonic stem cell biology." BRITISH SOCIETY FOR CELL BIOLOGY "... the imaginative solutions in this book can inspire us to get past our most frustrating limitations." CELL STEM CELL "... the richness in the details of each protocol presented will certainly encourage more scientists to begin studies of Human pluripotent stem cells..." REGENERATIVE MEDICINE "In this fast-moving field, this [handbook] will help drive advances of more and more researchers." DIFFERENTIATION "...a valuable resource for seasoned and novice researchers... an excellent addition to the reference collection of any medical library or research laboratory." THE AMERICAN MEDICAL ASSOCIATION
Human Embryonic Stem Cell Protocols
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Author : Turksen
language : en
Publisher:
Release Date : 2009-08-01
Human Embryonic Stem Cell Protocols written by Turksen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-08-01 with categories.
Guidelines For Human Embryonic Stem Cell Research
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Author : National Research Council
language : en
Publisher: National Academies Press
Release Date : 2005-09-15
Guidelines For Human Embryonic Stem Cell Research written by National Research Council and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-09-15 with Medical categories.
Since 1998, the volume of research being conducted using human embryonic stem (hES) cells has expanded primarily using private funds because of restrictions on the use of federal funds for such research. Given limited federal involvement, privately funded hES cell research has thus far been carried out under a patchwork of existing regulations, many of which were not designed with this research specifically in mind. In addition, hES cell research touches on many ethical, legal, scientific, and policy issues that are of concern to the public. This report provides guidelines for the conduct of hES cell research to address both ethical and scientific concerns. The guidelines are intended to enhance the integrity of privately funded hES cell research by encouraging responsible practices in the conduct of that research.
Embryonic Stem Cell Protocols
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Author : Kursad Turksen
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Embryonic Stem Cell Protocols written by Kursad Turksen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.
Human Embryonic And Induced Pluripotent Stem Cells
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Author : Kaiming Ye
language : en
Publisher: Humana Press
Release Date : 2016-08-23
Human Embryonic And Induced Pluripotent Stem Cells written by Kaiming Ye and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.
Because of the huge potential of human embryonic stem (hES) cells, especially the newly developed human induced pluripotent stem (hiPS) cells, in disease treatment and life quality improvement, enormous efforts have been made to develop new methodologies to translate lab discoveries in stem cell research into bed-side clinical technologies. In Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols, experts in the field present a comprehensive collection of protocols designed for labs around the world. The topics covered in this detailed volume include techniques used for maintenance of hES and iPS cells in either small or large scale, techniques for directing hES and iPS cell lineage specification, techniques for enhancing the maturity of differentiated hES and iPS cells within three-dimensional scaffolds, techniques for reprogramming adult cells into iPS cells, techniques for generating patient-specific iPS cells, and techniques for translating hES and iPS cell research into new therapies. Chapters include lab ready protocols with tips on troubleshooting and avoiding known pitfalls. Wide-ranging and authoritative, Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols will be a tremendous aid for researchers and students who wish to explore these areas and transform their discoveries into the next generation of regenerative medicine and tissue engineering technologies.
Culture Of Human Stem Cells
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Author : R. Ian Freshney
language : en
Publisher: John Wiley & Sons
Release Date : 2007-07-16
Culture Of Human Stem Cells written by R. Ian Freshney and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-07-16 with Science categories.
This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource. This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture. The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.
Embryonic Stem Cells
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Author : Michael S. Kallos
language : en
Publisher: BoD – Books on Demand
Release Date : 2011-09-15
Embryonic Stem Cells written by Michael S. Kallos and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-15 with Science categories.
Embryonic stem cells are one of the key building blocks of the emerging multidisciplinary field of regenerative medicine, and discoveries and new technology related to embryonic stem cells are being made at an ever increasing rate. This book provides a snapshot of some of the research occurring across a wide range of areas related to embryonic stem cells, including new methods, tools and technologies; new understandings about the molecular biology and pluripotency of these cells; as well as new uses for and sources of embryonic stem cells. The book will serve as a valuable resource for engineers, scientists, and clinicians as well as students in a wide range of disciplines.