Identifying Unknown Substrates Of S Adenosyl Methionine Dependent Methyltransferases Via In Situ Formation Of Tight Binding Bisubstrate Adducts
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Identifying Unknown Substrates Of S Adenosyl Methionine Dependent Methyltransferases Via In Situ Formation Of Tight Binding Bisubstrate Adducts
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Author : Wanlu Qu
language : en
Publisher:
Release Date : 2015
Identifying Unknown Substrates Of S Adenosyl Methionine Dependent Methyltransferases Via In Situ Formation Of Tight Binding Bisubstrate Adducts written by Wanlu Qu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with Adenosylmethionine categories.
Identification of enzyme substrates is an essential step toward understanding the physiological functions of enzymes. However, progress in substrate elucidation is hampered by the transient nature of enzyme-substrate interactions. This challenge is exemplified by S-adenosyl-methionine (AdoMet or SAM) dependent methyltransferases (MTases). AdoMet dependent MTases catalyze the methyl-transfer reaction from AdoMet to a wide array of nucleophilic substrates. These nucleophilic substrates, however, are only transiently associated to the enzyme. Inspired by the concept of multisubstrate adduct inhibition, a novel approach of enzyme-catalyzed in situ bisubstrate-adduct formation is presented for capturing substrates of MTases via tight binding MTase-adduct complexes. In our approach, the electrophilic methyl sulfonium in AdoMet is replaced with a vinyl sulfonium in S-adenosyl-vinthionine (AdoVin). AdoVin forms a covalent bisubstrate-adduct with the nucleophilic substrate and the resulting adduct tightly binds the enzyme. Using this strategy, an unknown substrate was readily identified in crude cell lysates, and was found to be a derivative of exogenous substrates. Another significant achievement is the detection of the complex between the bisubstrate-adduct and enzyme using native mass spectrometry directly from the reaction mixture, without prior purification even using crude cell lysates. The subsequent identification of substrates was achieved by employing various tandem mass spectrometric techniques, such as collision induced dissociation (CID) and surface induced dissociation (SID), in a single run. To the best of our knowledge, this is the first example of MTase substrate identification from cell lysates without any separation prior to mass spectrometric analysis. The approaches outlined here should be generally applicable for identifying substrates of other group transferases or enzymes sharing similar mechanisms.
Dna And Rna Modification Enzymes
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Author : Henri Grosjean
language : en
Publisher: CRC Press
Release Date : 2009-06-11
Dna And Rna Modification Enzymes written by Henri Grosjean and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-06-11 with Science categories.
This volume is a timely and comprehensive description of the many facets of DNA and RNA modification-editing processes and to some extent repair mechanisms. Each chapter offers fundamental principles as well as up to date information on recent advances in the field (up to end 2008). They ended by a short ‘conclusion and future prospect’ section and an exhaustive list of 35 to up to 257 references (in average 87). Contributors are geneticists, structural enzymologists and molecular biologists working at the forefront of this exciting, fast-moving and diverse field of researches. This book will be a major interest to PhD students and University teachers alike. It will also serve as an invaluable reference tool for new researchers in the field, as well as for specialists of RNA modification enzymes generally not well informed about what is going on in similar processes acting on DNA and vice-versa for specialists of the DNA modification-editing and repair processes usually not much acquainted with what is going on in the RNA maturation field. The book is subdivided into 41 chapters (740 pages). The common links between them are mainly the enzymatic aspects of the different modification-editing and repair machineries: structural, mechanistic, functional and evolutionary aspects. It starts with two general and historical overview of the discovery of modified nucleosides in DNA and RNA and corresponding modification-editing enzymes. Then follows eleven chapters on DNA modification and editing (mechanistic and functional aspects). Two additional chapters cover problems related to DNA/RNA repair and base editing by C-to-U deaminases, followed by three chapters on RNA editing by C-to-U and A-to-I type of deamination. Discussions about interplay between DNA and RNA modifications and the emergence of DNA are covered in two independent chapters, followed by twenty chapters on different but complementary aspects of RNA modification enzymes and their cellular implications. The last chapter concerns the description of the present state-of-the art for incorporating modified nucleosides by in vitro chemical synthesis. At the end of the book, six appendicies give useful details on modified nucleosides, modification-editing enzymes and nucleosides analogs. This information is usually difficult to obtain from current scientific literature.
Nutritional Biochemistry Of The Vitamins
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Author : David A. Bender
language : en
Publisher: Cambridge University Press
Release Date : 2003-09-18
Nutritional Biochemistry Of The Vitamins written by David A. Bender and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-09-18 with Science categories.
An authoritative and comprehensive review of our current knowledge of the vitamins, their metabolic functions and the scientific basis for setting recommended intakes for the prevention of deficiency and promotion of optimum health. This publication will be a valuable reference for students and specialists alike in the field of nutritional biochemistry.
Fundamentals Of Chromatin
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Author : Jerry L. Workman
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-12-04
Fundamentals Of Chromatin written by Jerry L. Workman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-04 with Medical categories.
While there has been an increasing number of books on various aspects of epigenetics, there has been a gap over the years in books that provide a comprehensive understanding of the fundamentals of chromatin. Chromatin is the combination of DNA and proteins that make up the genetic material of chromosomes. Its primary function is to package DNA to fit into the cell, to strengthen the DNA to prevent damage, to allow mitosis and meiosis, and to control the expression of genes and DNA replication. The audience for this book is mainly newly established scientists and graduate students. Rather than going into the more specific areas of recent research on chromatin the chapters in this book give a strong, updated groundwork about the topic. Some the fundamentals that this book will cover include the structure of chromatin and biochemistry and the enzyme complexes that manage it.
Basic Aspects Of Catechol O Methyltransferase And The Clinical Applications Of Its Inhibitors
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-11-20
Basic Aspects Of Catechol O Methyltransferase And The Clinical Applications Of Its Inhibitors written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-20 with Science categories.
Volume 95 of International Review of Neurobiology focuses on Catechol-O-methyltransferase inhibition, and its clinical application in relation to Parkinson’s disease. Chapters cover COMT gene and proteins, L-dopa treatment in Parkinson’s disease, the latest research on COMT inhibitors and their clinical applications, as well as future prospects for their use. Up-to-date summary of biochemistry and pharmacology of COMT and its inhibitors Preclinical models in COMT inhibitor devlopment Transgenic COMT mice – latest information summarized Chemistry of COMT inhibitors and their design with molecular modelling COMT gene and its regulation and relation to dopamine related diseases Role of cofactor SAM regulation in relation to homocysteine Nonclinical and clinical safety of COMT inhibitors summarized Future prospects of COMT inhibitors in Parkinson’s disease
Is Arsenic An Aphrodisiac
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Author : William R. Cullen
language : en
Publisher: Royal Society of Chemistry
Release Date : 2008
Is Arsenic An Aphrodisiac written by William R. Cullen and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Medical categories.
Sex, drugs, rocks, gold, murder, war, mass poisonings, the deaths of Napoleon, Tchaikovsky, Mozart, and others are all linked by one element - arsenic! Arsenic has been around since the beginning of time and the word has become a metaphor for poison with associated shock value. The general public are fearful of any possible exposure to it and yet it holds a certain dark and eerie fascination! The average person has only one idea about arsenic - it is poison - and this reputation has a sound base. Some arsenic compounds are very toxic and have been used with criminal intent from the time of the.
Advances In Medicinal Chemistry
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Author : B.E. Maryanoff
language : en
Publisher: Elsevier
Release Date : 1999-04-01
Advances In Medicinal Chemistry written by B.E. Maryanoff and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-04-01 with Science categories.
Volume 4 of Advances in Medicinal Chemistry is comprised of six chapters on a wide range of topics in medicinal chemistry, including molecular modeling, structure-based drug design, organic synthesis, peptide conformational analysis, biological assessment, structure-activity correlation, and lead optimization. Chapter 1 presents an account about amino acid-based peptide mimetics corresponding to b-turn, loop, helical motifs in proteins as a probe of ligand-receptor and ligand-enzyme molecular interactions. Chapter 2 addresses new facets of the medicinal chemistry of the important anticancer drug Taxol® (paclitaxel). Chapter 3 relates an account of the search for new drugs for the treatment of malaria based on the natural product artemisinin. Chapter 4 applies computational chemistry to the evaluation of compound libraries for biological testing. Chapter 5 describes the construction of a 3-dimensional molecular model of the human thrombin receptor, the first protease-activated G-protein coupled receptor (PAR-1), as a means to explore the intermolecular contacts involved in agonist peptide recognition. Finally, Chapter 6 describes the research conducted at Merck on inhibitors of farnesyl transferase as a potential treatment for human cancers.
Modified Nucleosides
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Author : Piet Herdewijn
language : de
Publisher: John Wiley & Sons
Release Date : 2008-09-08
Modified Nucleosides written by Piet Herdewijn and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-09-08 with Science categories.
Edited by one of the main driving forces behind the field's momentous rise in recent years, this one-stop reference is the first comprehensive resource to integrate recent advances. The first part addresses biochemical aspects and applications, the second and third parts are devoted to compounds with therapeutic potential, with the third part focusing on newly introduced anticancer nucleoside drugs. Essential reading for every scientist working in this area.
Glyco Engineering
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Author : Alexandra Castilho
language : en
Publisher: Humana
Release Date : 2015-06-17
Glyco Engineering written by Alexandra Castilho and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-06-17 with Science categories.
Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and the properties of proteins through manipulations on the production host rather than in the protein itself. The first five sections deal with host-specific glyco-engineering and contain chapters that provide protocols for modifications of the glycosylation pathway in bacteria, yeast, insect, plants and mammalian cells, while the last two sections explore alternative approaches to host glyco-engineering and selected protocols for the analysis of the N-glycans and glyco-profiling by mass spectrometry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and extensive, Glyco-Engineering: Methods and Protocols offers vast options to help researchers to choose the expression system and approach that best suits their intended protein research or applications.
S Adenosylmethionine Dependent Methyltransferases Structures And Functions
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Author : Robert M Blumenthal
language : en
Publisher: World Scientific
Release Date : 1999-07-23
S Adenosylmethionine Dependent Methyltransferases Structures And Functions written by Robert M Blumenthal and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-07-23 with Science categories.
This invaluable volume, written by an international group of scientists, presents an overview of the AdoMet-dependent methyltransferases, with special emphasis on structure-function relationships.S-adenosyl-L-methionine (AdoMet) is the second most commonly used enzyme cofactor after ATP. The AdoMet-dependent methyltransferases act on a wide variety of target molecules, including DNA, RNA, protein, polysaccharides, lipids and a range of small molecules.The well-conserved architecture of these enzymes, and the implications of this conservation for their evolutionary history, are major themes of this book. The thirteen chapters describe in detail the structures, enzyme kinetics and biological roles of the AdoMet-dependent methyltransferases from a wide range of cell types: plant, animal, bacterial and archaeal.