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Immobilized Enzymes For Industrial Reactors


Immobilized Enzymes For Industrial Reactors
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Immobilized Enzymes For Industrial Reactors


Immobilized Enzymes For Industrial Reactors
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Author : Ralph Messing
language : en
Publisher: Elsevier
Release Date : 2012-12-02

Immobilized Enzymes For Industrial Reactors written by Ralph Messing and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Technology & Engineering categories.


Immobilized Enzymes for Industrial Reactors aims to guide the engineer and scientist along the path toward the industrial application of immobilized enzymes. It is necessary to identify the hazards and pitfalls that will be encountered, not only in the initial research efforts, but also during the final engineering phases of a commercial program. Each contributing factor in an immobilized enzyme system will be scrutinized by the authors in an effort to accomplish the overall objectives. This book comprises 10 chapters, with the first being an introduction to and general history of immobilized enzymes. The next chapters go on to discuss basic enzymology; controlled-pore glasses for enzyme immobilization; carriers; immobilization by adsorption and inorganic bridge formation; immobilization by covalent attachment and by entrapment; characteristics of free vs. immobilized enzymes; immobilized coenzymes; design and operation of immobilized enzyme reactors; and applications of immobilized enzymes. This book will be of interest to practitioners in the fields of chemistry and engineering.



Analytical Applications Of Immobilized Enzyme Reactors


Analytical Applications Of Immobilized Enzyme Reactors
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Author : S. Lam
language : en
Publisher: Springer
Release Date : 1994

Analytical Applications Of Immobilized Enzyme Reactors written by S. Lam and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Medical categories.


Enzymes are bio-catalysts which effect transformation of substrates to products with high specificity. The usage of enzymes in domestic and industrial applications is well known and has been well documented since the early history of civilization. With the advances in understanding of enzymology, usage of enzymes in industrial and biotechnological pro cesses and molecular medicine has proliferated. One of the key factors in the widespread application of enzymes in modern technologies is the development of enzyme immobilization tech niques, which overcome certain practical, functional and economic con straints. Many natural enzymes can be stabilized by immobilization on solid matrices, with most of the activity retained, for a variety of applica tions. An important application of immobilized enzymes is in liquid chromatography. In the last decade, post-column enzyme detection has become established as an important discipline in liquid chromatography. The new detection approach offers more sensitive and specific ways for measuring major classes of biomolecules. Reactors are fabricated by packing the immobilized enzymes into small columns, which can be placed immediately after an HPLC column.



Immobilization Of Enzymes And Cells


Immobilization Of Enzymes And Cells
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Author : José M. Guisán
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05

Immobilization Of Enzymes And Cells written by José M. Guisán and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.


Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation—from the laboratory to industry—of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of protocols for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few protocols are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related properties.



Enzyme Immobilization


Enzyme Immobilization
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Author : Rowan Austin
language : en
Publisher: Scientific e-Resources
Release Date : 2018-12-19

Enzyme Immobilization written by Rowan Austin and has been published by Scientific e-Resources this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-12-19 with categories.


An enzyme is a protein, or protein complex, that catalyses a chemical reaction. Like any catalyst, enzymes work by lowering the activation energy of a reaction, thus allowing the reaction to proceed to its steady state or completion much faster than it otherwise would; the enzyme remains unaltered by the completed reaction and can therefore continue catalysis. An immobilized enzyme is an enzyme attached to an inert, insoluble material-such as calcium alginate. This can provide increased resistance to changes in conditions such as pH or temperature. It also lets enzymes be held in place throughout the reaction, following which they are easily separated from the products and may be used again-a far more efficient process and so is widely used in industry for enzyme catalysed reactions. An alternative to enzyme immobilization is whole cell immobilization. Carrier matrices for enzyme immobilisation by adsorption and covalent binding must be chosen with care. The manufacture of high-valued products on a small scale may allow the use of relatively expensive supports and immobilisation techniques whereas these would not be economical in the large-scale production of low added-value materials. A substantial saving in costs occurs where the carrier may be regenerated after the useful lifetime of the immobilised enzyme. This book is simple protocols for the immobilization of enzymes and cells that could be useful for application at industrial scale, novel protocols for immobilization in the future, and new chemical reactors able to overcome the limitations of a number of immobilized derivatives.



Immobilized Enzymes


Immobilized Enzymes
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Author : Michael D. Trevan
language : en
Publisher: John Wiley & Sons
Release Date : 1980

Immobilized Enzymes written by Michael D. Trevan and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1980 with Science categories.




Analytical Uses Of Immobilized Biological Compounds For Detection Medical And Industrial Uses


Analytical Uses Of Immobilized Biological Compounds For Detection Medical And Industrial Uses
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Author : George G. Guilbault
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Analytical Uses Of Immobilized Biological Compounds For Detection Medical And Industrial Uses written by George G. Guilbault and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.


On May 4-8, 1987, a NATO Advanced Research Workshop on the Analytical Uses of Immobilized Biological Compounds was held in Florence, Italy. The Director of the Workshop was Professor George G. Guilbault of the University of New Orleans, and the Co-Director was Professor Marco Mascini of the University of Florence It vas the purpose of this meeting to assemble scientists from all NATO Countries with an interest in immobilized biological compounds. to discuss - methods of immobilization - properties of immobilized compounds - enzyme electrodes and biosensors - optical devices utilizing immobilized enzymes - microbial sensors and clinical uses of immobilized enzymes - flow injection analysis using enzymes - immobilized biological compounds in chemical defense detection - pharmaceutical analysis - uses in industrial analysis - enzyme reactors - air pollution detectors - immunosensors - medical uses and applications - solid state and FET sensors Goals to be achieved by the conference were - to permit an exchange of views and experience in all these areas - to review and critically assess the state-of-the-art in these fields - to set guidelines for future research and establish collaborative projects between scientists in NATO laboratories in the above areas. Thirty-seven lectures were given by 36 speakers in all of the above areas.



Immobilized Enzymes For Food Processing


Immobilized Enzymes For Food Processing
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Author : Pitcher
language : en
Publisher: CRC Press
Release Date : 2019-08-08

Immobilized Enzymes For Food Processing written by Pitcher and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-08 with Science categories.


Much has been written about immobilized enzymes during this period of time. So much, in fact, that it can become difficult even for those involved in developing new enzymatic food processing operations to bridge the gap between the field of immobilized enzymes and their specific requirements. It is the purpose of this book to assist those engaged in this difficult task. It is also a goal to bring to the researcher in enzyme immobilization an appreciation for the requirements of the food processing industry.



Immobilized Enzymes In Food And Microbial Processes


Immobilized Enzymes In Food And Microbial Processes
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Author : Alfred Olson
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11

Immobilized Enzymes In Food And Microbial Processes written by Alfred Olson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.


In the last five years the potential value of immobilized enzymes has captured the imagination of an increasing number of scientists and engineers. The concept of being able to create an immobilized derivative of an enzyme which has long-term stability and is able to be recovered and reused is fascinating, to say the least. Since the industrial application of enzymes has been mostly in the food and microbial process industry it is not surprising that many of the applications of immobilized enzymes considered for commercial development fall within the area of this industry. It is for this reason that we organized a symposium on immobilized enzymes for the 166th National Meeting of the American Chemical Society. Appropriately, the symposium was jointly sponsored by the Division of Agricultural and Food Chemistry and the Division of Microbial Chemistry and Technology. Although there were at least half a dozen symposia at other meetings on various aspects of immobilized enzyme technology in the preceding ten months none had specifically addressed themselves to food and microbial processes and none had been held at a meeting such as the National ACS gathering, which is not only large but multidisciplinary. The enthusiastic response to this symposium prompted us, at the invita tion of Plenum Press, to publish the proceedings of this symposium.



Enzyme Biocatalysis


Enzyme Biocatalysis
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Author : Andrés Illanes
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-06-19

Enzyme Biocatalysis written by Andrés Illanes and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-06-19 with Technology & Engineering categories.


This book was written with the purpose of providing a sound basis for the design of enzymatic reactions based on kinetic principles, but also to give an updated vision of the potentials and limitations of biocatalysis, especially with respect to recent app- cations in processes of organic synthesis. The ?rst ?ve chapters are structured in the form of a textbook, going from the basic principles of enzyme structure and fu- tion to reactor design for homogeneous systems with soluble enzymes and hete- geneous systems with immobilized enzymes. The last chapter of the book is divided into six sections that represent illustrative case studies of biocatalytic processes of industrial relevance or potential, written by experts in the respective ?elds. We sincerely hope that this book will represent an element in the toolbox of gr- uate students in applied biology and chemical and biochemical engineering and also of undergraduate students with formal training in organic chemistry, biochemistry, thermodynamics and chemical reaction kinetics. Beyond that, the book pretends also to illustrate the potential of biocatalytic processes with case studies in the ?eld of organic synthesis, which we hope will be of interest for the academia and prof- sionals involved in R&D&I. If some of our young readers are encouraged to engage or persevere in their work in biocatalysis this will certainly be our more precious reward.



Immobilized Enzyme Reactors


Immobilized Enzyme Reactors
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Author : Stephen Gregory Moore
language : en
Publisher:
Release Date : 1982

Immobilized Enzyme Reactors written by Stephen Gregory Moore and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1982 with Catalysts categories.