Immunoassays
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Immunoassay
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Author : Brian Law
language : en
Publisher: CRC Press
Release Date : 2002-09-11
Immunoassay written by Brian Law and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-09-11 with Medical categories.
Immunoassay development is a multidisciplinary activity involving a wide range of skills possessed by few laboratories. This presentation of tried and tested methods should enable scientists and researchers in the pharmaceutical and related industries to more rapidly and effectively develop immunoassays upon which their work is becoming heavily dependent.; Important methods are included for preparing Lapten-protein conjugates and raising the necessary antibodies, concentrating on polyclonal sera, as well as methods for the synthesis of radio and enzyme labelled tracers. Particular attention is paid to the requirements of the regulatory authorities such as the FDA (Food and Drug Administration) with respect to assay validation. Further chapters deal with assay development and optimization, curve fitting and quality control procedures.
Enzyme Immunoassays
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Author : S.S. Deshpande
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Enzyme Immunoassays written by S.S. Deshpande and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
This unique reference provides a pragmatic approach to the development of successful commercial immunodiagnostic products based on enzyme immunoessay technology. Presenting both the basic and applied principles, Enzyme Immunoassays gathers information on all aspects of this process, from the initial conceptualization to the introduction of the product to the market.
Nonisotopic Immunoassay
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Author : That T. Ngo
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Nonisotopic Immunoassay written by That T. Ngo and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The basis of all immunoassays is the interaction of antibodies with antigens. The most widely used immunoassay technique is radioimmunoassay (RIA) which was first developed by Yalow and Berson in 1959. The principle of RIA is elegantly simple. It utilizes a competitve binding reaction between analytes and a radio-labeled analog of the analytes (the tracer) for anti-analyte antibodies. In addition to its exquisite specificity, extraordinary sensitivity, good accuracy and precision, ease and rapidity of assay and simplicity of assay development, the applicability of RIA to a wide variety of substances has made it one of the most powerful and versatile analytical methods of the 20th century and beyond. Millions of RIA's are being performed annually on clinical, biological and environmental samples in licensed laboratories. In order to expand the use of RIA beyond the confines of these laboratories to areas like physician's offices, patients' homes, economically less developed countries, agricultural fields, large scale and continuing screening tests for infectious diseases, it has become necessary to develop non-isotopic labels. Indeed the last fifteen years have seen the development of a great number of ingenious non-isotopic labels in immunoassay so that a whole new industry capitalizing on the potential market for non isotopic immunoassays has appeared. It is the purpose of this volume to present in depth, state-of-the-art reviews on techniques used in non-isotopic immunoassays. Topics covered include: (1) Enzyme-labeled immunoassay; (2) Luminescene immunoassay; (3) Immunoassay at liquid-solid interface; (4) Membrane immunoassay and (5) "Particle"-mediated immunoassay.
Principles And Practice Of Immunoassay
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Author : Christopher P. Price
language : en
Publisher: Springer
Release Date : 1991-06-18
Principles And Practice Of Immunoassay written by Christopher P. Price and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991-06-18 with Medical categories.
Each chapter of this book aims to explore the basic physical and chemical principles involved in the immunoassay techniques discussed. The book also looks at the optimization and limitations of methodology and concludes with a brief overview of the application of the performance of the technology.
Immunoassays
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Author : Richard O'Kennedy
language : en
Publisher: CRC Press
Release Date : 2017-06-26
Immunoassays written by Richard O'Kennedy and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-06-26 with Medical categories.
The concept behind this book is to provide a detailed and practical overview of the development and use of immunoassays in many different areas. Immunoassays are analytical tests that utilise antibodies to measure the amount, activity or identity of an analyte. This book is designed to provide a critical and helpful insight into the subject and to give the user practical information that may be of assistance in assay format selection, antibody generation/selection and choice of appropriate detection strategies. It is comprised of 13 chapters written by highly experienced researchers in the fields of antibody-based research, immunoassay development, assay validation, diagnostics and microfluidics. Beginning with a comprehensive survey of antibodies, immunoassay formats and signalling systems, the book elucidates key topics related to the development of an ideal antibody-based sensor, focuses on the important topic of surface modification, explores key parameters in the immobilisation of antibodies onto solid surfaces, discusses the move to ‘lab-on-a-chip’-based devices and investigates the key parameters necessary for their development. Three of the chapters are dedicated to the areas of clinical diagnostics, infectious disease monitoring and food security, where immunoassay-based applications have become highly valuable tools. The future of immunoassays, including next-generation immunoassays, electrochemical-immunoassays and ‘lab-on-a-chip’-based systems, is also discussed. The book also covers the use of optical detection systems (with a focus on surface plasmon resonance) in immunoassays, provides a compilation of important, routinely used immunoassay protocols and addresses problems that may be encountered during assay development.
Immunoassay
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Author : Eleftherios P. Diamandis
language : en
Publisher: Academic Press
Release Date : 1996-06-21
Immunoassay written by Eleftherios P. Diamandis and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-06-21 with Medical categories.
Immunoassays are among the most powerful and sensitive technologies now available for patient diagnosis and monitoring. This book is an indispensable guide to information on the theory and practice of immunoassays. It discusses the scientific basis of these technologies in a logical, organized, and heuristic manner and provides protocols for specific assays. The contents of this unique book are balanced among theory, practical issues, quality control, automation, and subspecialty areas, making it ideal for health science students, laboratory scientists, and clinicians. Presents up-to-date information Provides extensive cross-referencing Covers theory and practice in full detail Written by leading authorities
Practice And Theory Of Enzyme Immunoassays
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Author : P. Tijssen
language : en
Publisher: Elsevier
Release Date : 1985-08-01
Practice And Theory Of Enzyme Immunoassays written by P. Tijssen and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985-08-01 with Medical categories.
Enzyme immunoassays have developed into a powerful assay technology, transcending several discipline boundaries, extensively applied as a tool in fields other than enzymology and immunology. This volume reflects the rapid progress in the applications of this technique, providing a basic understanding of these techniques and a practical guideline for the choice and experimental detail.
The Immunoassay Handbook
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Author : David Wild
language : en
Publisher: Elsevier
Release Date : 2005-06-20
The Immunoassay Handbook written by David Wild and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-06-20 with Medical categories.
Containing updated and new information on advanced technology - including micro and nanoscale immunoassays - this text provides a mix of practical information coupled with a review of clincal applications and practical examples.
Elisa And Other Solid Phase Immunoassays
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Author : D. M. Kemeny
language : en
Publisher: John Wiley & Sons
Release Date : 1991-01-08
Elisa And Other Solid Phase Immunoassays written by D. M. Kemeny and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991-01-08 with Science categories.
This is a quick-reference manual on practical aspects of immunoassay. Providing a theoretical and practical basis for modern applications of solid-phase immunoassays, this text brings together experts who have used ELISA and other assays in a variety of fields. Contributors offer step-by-step guidance on how to use the various techniques involved in immunoassay. These techniques are extemely useful to laboratory-based researchers and technicians working on the detection of allergy, the AIDS virus, autoimmunity, etc. Chapters analyze the solid-phase supports used, the amplification systems, and the quantitation and affinity of antibodies and discuss the applications of assays to biology, immunology, and microbiology.
Immunoassays
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Author : James P. Gosling
language : en
Publisher: Oxford University Press, USA
Release Date : 2000
Immunoassays written by James P. Gosling and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Medical categories.
Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents, standards and immunogens are explained, with procedures for their preparation. But most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.