In Situ Hybridization In Brain
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In Situ Hybridization In Brain
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Author : George Uhl
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
In Situ Hybridization In Brain written by George Uhl and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The explosion of interest in specific molecules important for brain function and dysfunction has drawn individuals from diverse backgrounds toward the use of in situ hybridization techniques. Study of the brain demands the anatomic precision and biochemical specificity that this approach can potentially bring. Workers with backgrounds in peptide neuroanatomy, neuropharmacology, molecular biology, neurovirology, neuropathology, and neurophysiology have joined together in this volume to discuss their initial experiences in applying ill situ hybridization techniques to the study of the brain. The work, although still in an early phase of development, is worthy of initial summary and dissemination. In the area of neuropeptide gene expression alone, investigators represented here describe studies of vasopressin, opiate peptides, oxytocin, vasoactive intestinal peptide, cholecystokinin, and somatostatin. Other contributions provide insight into applications of the technique to studies of the expression of genes for neurotransmitter synthesizing enzymes, viral-encoded genes, trophic factor genes, and the genes selected on the basis of their special roles in the brain. The authors provide an important series of technical perspectives, and describe specific experimental protocols. This volume should be of interest to individuals seeking an introduction to these methods, as well to those desiring an up to date precis of work in this burgeoning area. Dr. Uhl, with the sponsorship of the Howard Hughes Medical Institute, has done a superb job of assembling the leaders in this area, and in organizing the presen ta tion of their perspecti ves herein. Joseph B. Martin, M.D., Ph.D.
In Situ Hybridization Protocols For The Brain
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Author : W. Wisden
language : en
Publisher: Gulf Professional Publishing
Release Date : 2002-08-05
In Situ Hybridization Protocols For The Brain written by W. Wisden and has been published by Gulf Professional Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-08-05 with Medical categories.
This volume of the International Review of Neurobiology was written to assist researchers without any previous experience with in situ hybridization, allowing them to follow the protocols and expect good results. It contains all the information required for newcomers to achieve successful in situ hybridization results, and methods for improving the technique of those already utilizing it. Published since 1959, International Review of Neurobiology is a well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists. Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research. A well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research
In Situ Hybridization Techniques For The Brain
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Author : Z. Henderson
language : en
Publisher:
Release Date : 1996-12-11
In Situ Hybridization Techniques For The Brain written by Z. Henderson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-12-11 with Medical categories.
Written by molecular biologists and neurobiologists, this volume in the Handbook Series provides basic step-by-step protocols in addition to specialist techniques to support those practising in situ hybridization, a powerful histochemical technique which is now widely used in neurobiology. The first part of the manual concentrates on basic procedures, eg, which probe and label to select, equipment, relevant molecular biological techniques, hazards, probe preparation and labelling, tissue preparation, and conditions for hybridization. Specialist procedures including quantitative analysis of data, reverse transcription PCR, working with human tissue, and double label methods are presented in subsequent sections. In Situ Hybridization Techniques for the Brain is, therefore, an essential reference source for all those with research interests in neuroanatomy, histopathology and/or neurohistochemistry, and will support users in both the preparation and successful completion of the procedures in their own laboratory.
In Situ Hybridization
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Author : Karen L. Valentino
language : en
Publisher: Oxford University Press, USA
Release Date : 1987
In Situ Hybridization written by Karen L. Valentino and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Language Arts & Disciplines categories.
A technique for reading the messenger RNAs by which cells express their distinctive populations of structural and functional proteins, in situ hybridization is now reliable and practical for use in neurobiological research.
In Situ Hybridization Histochemistry
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Author : Marie-Francoise Chesselet
language : en
Publisher: CRC Press
Release Date : 1990-07-03
In Situ Hybridization Histochemistry written by Marie-Francoise Chesselet and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-07-03 with Medical categories.
The goal of this fascinating new book is to review the diversity of methods available to apply in situ hybridization histochemistry (ISHH) to a variety of experimental questions. This work includes topics such as synthesis and use of nick-translated DNA probes for ISHH, synthesis and use of oligomeric DNA probes for ISHH, and synthesis and use of RNA probes for ISHH. These interesting chapters describe the preparation of different radiolabeled probes for ISHH. They also discuss their respective advantages and limitations, and describe current results based on the use of these various probes. Sections of the text highlight low and high resolution autoradiography for ISHH, the use of biotin-labeled probes for ISHH, as well as the use of ISHH in combination with established anatomical techniques. In Situ Hybridization Histochemistry answers all of your questions regarding the quantification of ISHH. It also provides a practical description of typical protocols, both from molecular biology and histology. Investigators will understand and value this useful, powerful tool-whatever their backgrounds might be.
Molecular Neuroanatomy
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Author : Fred W. Leeuwen
language : en
Publisher: Elsevier Publishing Company
Release Date : 1988
Molecular Neuroanatomy written by Fred W. Leeuwen and has been published by Elsevier Publishing Company this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with Medical categories.
For a thorough study of the dynamics of particular brain compounds it is now possible to use and combine various molecular neuroanatomical methods (e.g. in situ hybridization, receptor localisation and immunocytochemistry) in a quantitative way on whole brain sections maintaining morphological details. Molecular Neuroanatomy deals with the many practical aspects and recent developments in these areas. The theoretical background of many techniques is presented, as well as clear, step-by-step instructions on the preparation and application of all the methods and techniques described in this book. It will be invaluable to all those working in the field of neuroscience. Available in both hardback and paperback, with colour illustrations.
In Situ Hybridization Medical Applications
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Author : G.R. Coulton
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
In Situ Hybridization Medical Applications written by G.R. Coulton and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
In situ hybridization has developed as a means of localizing specific DNA and RNA sequences within tissues. The great strength of this approach is the ability to relate the distribution of specific nucleic acids with cell structures and the protein products of the target gene by means of immunohistochemistry. Complementary DNA, RNA or oligonucleotide probes, suitably labelled, are hybridized to specific DNA or RNA targets within tissues. The spatial information thus obtained has contributed greatly to our understanding of such diverse areas of research as gene mapping, viral infection, cytogenetics, protein synthesis, prenatal diagnosis and tissue grafting. This book is not intended as another recipe book, although it does describe theoretical and practical aspects of the technology. Rather, the authors critically describe the contribution made by in situ hybridization to specific areas of medical research.
Brain Development
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Author : Simon G Sprecher
language : en
Publisher: Humana
Release Date : 2016-09-29
Brain Development written by Simon G Sprecher and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-09-29 with Medical categories.
Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes. Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models. Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.
Microrna Profiling
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Author : Sweta Rani
language : en
Publisher: Springer Nature
Release Date : 2022-11-28
Microrna Profiling written by Sweta Rani and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-11-28 with Science categories.
This second edition provides updated and comprehensive methods on miRNA biogenesis and their role in the development and progression of various human diseases. Chapters detail miRNA biogenesis, isolating RNA, extracellular vesicles (EVs), circulating miRNAs, analyzing miRNA and miRDeep-P2, protocols for total RNA isolation from cells, cell-derived products, isolation and characterization of exosomes, serum, plasma specimens, and software tools. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MicroRNA Profiling: Methods and Protocols, Second Edition aims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientist.
In Situ Hybridization Methods
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Author : Giselbert Hauptmann
language : en
Publisher: Humana
Release Date : 2016-10-05
In Situ Hybridization Methods written by Giselbert Hauptmann and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-10-05 with Medical categories.
This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.