In Situ Hybridization In Light Microscopy

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In Situ Hybridization In Light Microscopy

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Author : Gerard Morel
language : en
Publisher: CRC Press
Release Date : 2000-09-28

In Situ Hybridization In Light Microscopy written by Gerard Morel and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-09-28 with Science categories.

In Situ hybridization allows the visualization of specific DNA/RNA sequences in individual cells in tissue sections, single cells, or chromosome preparations, and is an especially important method for studying DNA and RNA in heterogeneous cell populations. This book delves into in situ hybridization methods through the use of light microscopy used

Immunocytochemistry And In Situ Hybridization In The Biomedical Sciences

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Author : Julian E. Beesley
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Immunocytochemistry And In Situ Hybridization In The Biomedical Sciences written by Julian E. Beesley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

Immunocytochemistry and in situ hybridization are widely used biomedical sciences. They are essential in medical diagnosis and in cell biology research. Affinity labeling is the central goal of the experimental strategy involving a series of techniques in a logical order; from the effects of specimen fixation, through specimen preparation to expose the antigen, to optimizing immunolabeling, to assessing the result and finally to safety considerations. Numerous examples of these techniques in biomedical sciences are included, as well as experimental assays and practical tips. This survey of methods will serve as an invaluable reference source in any laboratory setting (academic, industrial or clinical) involved in research in almost every branch of biology or medicine, as well as in pharmaceutical, biotechnological and clinical applications.

Chromosome In Situ Hybridization Manual

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Author : Oncor Inc
language : en
Publisher:
Release Date : 1995

Chromosome In Situ Hybridization Manual written by Oncor Inc and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with categories.

In Situ Hybridization

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Author : David G. Wilkinson
language : en
Publisher: OUP Oxford
Release Date : 1998-11-26

In Situ Hybridization written by David G. Wilkinson and has been published by OUP Oxford this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-11-26 with Science categories.

In situ hybridization is used to reveal the location of specific nucleic acids sequences on chromosomes or in tissues. Visualization of the location of genes on chromosomes or of specific mRNAs or viruses in tissues is crucial for understanding the organization, regulation, and function of genes. It is a therefore a core technique in all areas of biomedical research. In Situ Hybridization: A Practical Approach 2/e is the second edition of one of the most successful Practical Approach books, published in 1992. Since the first edition was published, a number of important technical advances have been made. The new edition has been thoroughly updated to contain protocols detailing the major techniques of in situ hybridization currently in use: in situ hybridization to mRNA with oligonucleotide and RNA probes (radiolabelled and hapten labelled); analysis using light and electron microscopes; whole mount in situ hybridization; double detection of RNAs, and RNA plus protein; and fluorescent in situ hybridization to detect chromosomal sequences. The protocols are complemented by advice on strategies for successful results, descriptions of the theoretical basis of in situ hybridization and important new developments in gene expression databases. The procedures described are widely applicable to many systems. The use of in situ hybridization in PCR is covered in a separate volume: Herrington and O'Leary (Eds) PCR 3 - PCR in situ hybridization: A Practical Approach (OUP, 1997). All the authors have extensive practical experience of establishing reliable techniques of in situ hybridization. This book will be useful to all researchers at all levels who use in situ hybridization.

Situ Hybridization

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Author : LEITCH
language : en
Publisher: Springer
Release Date : 1994-06-30

Situ Hybridization written by LEITCH and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-06-30 with Science categories.

This practical guide provides a comprehensive description of in situ hybridization, from background information to detailed and practical applications. In situ hybridization is a powerful link between cellular and molecular biology, and can be used to localize nucleic acid sequences.

Fluorescence In Situ Hybridization Fish

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Author : Gal Haimovich
language : en
Publisher: Springer Nature
Release Date :

Fluorescence In Situ Hybridization Fish written by Gal Haimovich and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.

Fluorescence In Situ Hybridization Fish Application Guide

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Author : Thomas Liehr
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-11-26

Fluorescence In Situ Hybridization Fish Application Guide written by Thomas Liehr and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-26 with Science categories.

This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.

Fluorescence Microscopy

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Author : B. Herman
language : en
Publisher: Taylor & Francis
Release Date : 2020-08-26

Fluorescence Microscopy written by B. Herman and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-08-26 with Science categories.

This book covers the fundamental principles of fluorescence and their application to fluorescence microscopy, and presents applications to immunofluorescence, in situ hybridization, and photomicrography. It provides troubleshooting guidance to guide the user through commonly encountered problems.

Imaging Of Nucleic Acids And Quantitation In Photonic Microscopy

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Author : Xavier Ronot
language : en
Publisher: CRC Press
Release Date : 2001-05-30

Imaging Of Nucleic Acids And Quantitation In Photonic Microscopy written by Xavier Ronot and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-05-30 with Science categories.

Visualization of nuclear components has added tremendously to our understanding of the nucleus and its chemistry. The fluorescence approach is considered the present-day standard for comprehending nuclear organization. Imaging of Nucleic Acids and Quantitation in Photonic Microscopy provides new technologies in fluorescence microscopy and image cyt

Pcr Rt Pcr In Situ

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Author : Gerard Morel
language : en
Publisher: CRC Press
Release Date : 2002-09-27

Pcr Rt Pcr In Situ written by Gerard Morel and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-09-27 with Science categories.

Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application is the only method that allows the localization of amplified signal within tissue structure. The applications of in situ polymerase chain reaction have greatly enhanced the field of investigation in many disc