In Vitro Interactions Of Tubulin And Microtubules
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In Vitro Interactions Of Tubulin And Microtubules
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Author : Qingqin Li
language : en
Publisher:
Release Date : 1995
In Vitro Interactions Of Tubulin And Microtubules written by Qingqin Li and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with categories.
In Vitro Interactions Of Gamma Tubulin And Microtubules
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Author : Qingqin Li
language : en
Publisher:
Release Date : 1995
In Vitro Interactions Of Gamma Tubulin And Microtubules written by Qingqin Li and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with categories.
The In Vitro Interactions Between Tubulin And Hiv 1 Rev Require Rev S Multimerization And Arginine Rich Motifs
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Author : Amit Sharma
language : en
Publisher:
Release Date : 2009
The In Vitro Interactions Between Tubulin And Hiv 1 Rev Require Rev S Multimerization And Arginine Rich Motifs written by Amit Sharma and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with HIV (Viruses) categories.
Rev is a 13 kDa regulatory HIV protein essential for viral replication. It trans-activates expression of late viral proteins by multimerizing onto target mRNA and promoting their export into the cytoplasm. During attempts to find the solution conditions needed to study Rev structure, Watts et al. (2000) discovered that Rev depolymerizes microtubules (MTs) in vitro through the formation of ringed tubulin intermediates called Rev-tubulin toroids (RTTs). Rev interactions with MTs are specific and are thought to mimic the mechanism of Kinesin-13 proteins, themselves potent MT depolymerases that regulate the assembly of the mitotic spindle. If Rev and Kinesin-13 proteins share a common mechanism, then Rev mediated MT depolymerization and RTT formation will require Rev multimerization and its arginine-rich motif (ARM). If Rev multimerization is essential, then multimerization defective mutants should not depolymerize MTs and form RTTs. To test this hypothesis, a combination of sedimentation, gel filtration and visual assays were used to compare the activities of wild-type Rev with the multimerization defective mutant Rev M4 (M4). Both wild-type Rev and M4 are able to bind tubulin heterodimers and form high molecular complexes. However, these complexes are not RTTs. M4 also binds GMPCPP-stabilized MTs but unlike wild-type Rev, it neither depolymerizes MTs nor forms RTTs. These data show that Rev multimerization is important for MT depolymerization although it is unclear whether it is involved in targeting Rev to MT ends or provides the force required for depolymerization. Because M4 promotes MT bundling, this mutation is concluded to subtly affect Rev tertiary structure such that the relative orientation of Rev monomers within a multimer is altered in a manner that allows MT cross-linking. These same assays were also used to test the hypothesis that the ARM is also important for Rev-tubulin interactions. In this instance, the activities of wild-type Rev were compared to the M6 mutant (M6), a well-characterized substitution-deletion mutant in the ARM predicted to perturb binding, depolymerization and RTT formation. The M6 mutation affects RTT formation when mixed with tubulin heterodimers, reducing both ring size and the amount of complexes that can be sedimented. In addition, M6 is unable to depolymerize GMPCPP-stabilized MTs through an apparent inability to bind MT. Results presented here suggest Rev possesses only a single MT binding motif present in the ARM. Some residues in this region are critical for binding MT ends where depolymerization occurs. These conclusions are also consistent with the hypothesis that Rev interacts with MTs by a mechanism shared with Kinesin-13 proteins, themselves potent cellular MT depolymerases.
Microtubules In Vitro
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Author : John J. Correia
language : en
Publisher: Academic Press
Release Date : 2010-07-03
Microtubules In Vitro written by John J. Correia and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-07-03 with Science categories.
There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This volume (complemented by the forthcoming companion volume by Cassimeris and Tran) brings together current research and protocols in the field of microtoubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors. Chapters reflect both experimental procedures and new developments in the field of microtubule in vitro research Combines classical approaches and modern technologies Presents easy-to-use protocols and thorough background information, compiled by leaders in the field
Microtubule Dynamics
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Author : Anne Straube
language : en
Publisher: Humana Press
Release Date : 2017-04-30
Microtubule Dynamics written by Anne Straube and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-04-30 with Science categories.
Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field.
Interaction Of Anti Microtubule Agents With Tubulin In Vitro And In Stentor Coeruleus
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Author : Joanne Keene Kelleher
language : en
Publisher:
Release Date : 1976
Interaction Of Anti Microtubule Agents With Tubulin In Vitro And In Stentor Coeruleus written by Joanne Keene Kelleher and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1976 with Polymerization categories.
Modulation Of Microtubule Associated Protein Interactions With Microtubules And Actin In Vitro
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Author : Paul Steven Yamauchi
language : en
Publisher:
Release Date : 1987
Modulation Of Microtubule Associated Protein Interactions With Microtubules And Actin In Vitro written by Paul Steven Yamauchi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with categories.
Interactions Of Microtubule Associated Protiens Between Microtubules And Neurofilaments In Vitro
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Author : Gregory Charles Flynn
language : en
Publisher:
Release Date : 1988
Interactions Of Microtubule Associated Protiens Between Microtubules And Neurofilaments In Vitro written by Gregory Charles Flynn and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with categories.
Microtubules In Vitro
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Author : John J. Correia
language : en
Publisher: Academic Press
Release Date : 2013-08-20
Microtubules In Vitro written by John J. Correia and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-08-20 with Science categories.
There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This Second Edition brings together current research and protocols in the field of microtubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors. Chapters reflect experimental procedures and new developments in the field of microtubule in vitro research Combines classical approaches and modern technologies Presents easy-to-use protocols and thorough background information, compiled by leaders in the field
Gtp Interactions During In Vitro Tublin Polymerization And In Vivo Microtubule Assembly
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Author : James Michael Angelastro
language : en
Publisher:
Release Date : 1991
Gtp Interactions During In Vitro Tublin Polymerization And In Vivo Microtubule Assembly written by James Michael Angelastro and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with categories.