In Vitro Transcription And Translation Protocols
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In Vitro Transcription And Translation Protocols
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Author : Martin J. Tymms
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
In Vitro Transcription And Translation Protocols written by Martin J. Tymms and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Most laboratories conducting studies that use molecular biology techniques employ in vitro transcription and translation systems as a routine part of their day-to-day research. The commercial availability of purified bacterial RNA polymerase and the availability of robust tra- lation systems has made in vitro systems attractive not only as an alt- native to the in vivo expression of genes, but also as good model systems for studying specific aspects of transcription and translation. Although fairly efficient eukaryotic translation systems have been established for a number of years, reconstitution of transcription in vitro has proved to be more difficult. Recent improvements in fractionation techniques and the cloning of proteins involved in transcription have made this a fast moving area of research. Considerable progress has also been made in recent years in developing in vitro systems to study transcription and translation in chloroplasts and mitochondria, together with systems for the study of protein import. In Vitro Transcription and Translation Protocols provides many detailed experimental procedures for prokaryotic transcription and translation systems, together with protocols for many key techniques used in the analysis of eukaryotic transcription. In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, step-by-step instructions are provided, together with extensive notes that cover troubleshooting and special tips considered important.
In Vitro Transcription And Translation Protocols
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Author : Guido Grandi
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-05-03
In Vitro Transcription And Translation Protocols written by Guido Grandi and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-05-03 with Medical categories.
This book is a highly anticipated update of the previous edition. It provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. It has been completely updated with new chapters and topics.
In Vitro Transcription And Translation Protocols
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Author : Guido Grandi
language : en
Publisher: Humana Press
Release Date : 2007-05-03
In Vitro Transcription And Translation Protocols written by Guido Grandi and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-05-03 with Science categories.
A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. Completely updated with new chapters and topics, there is in-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems. Detailed protocols for protein production using different in vitro transcription and translation systems are included.
A Laboratory Guide To In Vitro Transcription
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Author : F. Sierra
language : en
Publisher: Birkhäuser
Release Date : 2013-11-21
A Laboratory Guide To In Vitro Transcription written by F. Sierra and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-21 with Science categories.
Methods In Molecular Biology In Vitro Transcription Protocols
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Author : John M. Walker
language : en
Publisher:
Release Date : 1984
Methods In Molecular Biology In Vitro Transcription Protocols written by John M. Walker and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols categories.
Protein Expression
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Author : S. J. Higgins
language : en
Publisher: OUP Oxford
Release Date : 1999-05-20
Protein Expression written by S. J. Higgins and has been published by OUP Oxford this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-05-20 with Science categories.
Protein Expression: A Practical Approach and its companion volume Post-translational Modification: A Practical Approach complete the mini-series of Practical Approach books covering the synthesis and subsequent processing of proteins. Protein Expression: A Practical Approach details the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. Cell-free systems of both eukarytotes and prokaryotes are described, including the prokaryotic systems that offer copuled transcription- translation. There is also a chapter on monitoring protein expression. The post- translational fate of proteins is covered in Post-Translational Processing: A Practical Approach.
Molecular Biomethods Handbook
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Author : Ralph Rapley
language : en
Publisher: Springer
Release Date : 2007-10-09
Molecular Biomethods Handbook written by Ralph Rapley and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-09 with Science categories.
An authoritative team of investigators illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced scientists fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included. The goal is to provide established scientists and novices who are new to these techniques with a deeper understanding of the widest variety of biotechniques and their applications.
Cell Free Protein Synthesis
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Author : Alexander S. Spirin
language : en
Publisher: John Wiley & Sons
Release Date : 2014-08-15
Cell Free Protein Synthesis written by Alexander S. Spirin and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-08-15 with Science categories.
With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.
Techniques In Molecular Biology
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Author : J.M. Walker
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Techniques In Molecular Biology written by J.M. Walker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.
In Vitro Mutagenesis Protocols
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Author : Jeff Braman
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
In Vitro Mutagenesis Protocols written by Jeff Braman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
Hands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications that allow high throughput mutagenesis experiments, and mutagenesis based on gene disruption (both in vitro- and in situ-based). Additional methods are provided for in vitro gene evolution; for gene disruption based on recombination, transposon, and casette mutagenesis; and for facilitating the introduction of multiple mutations. Time-tested and highly practical, the protocols in In Vitro Mutagenesis Protocols, 2nd Edition offer today's molecular biologists reliable and powerful techniques with which to illuminate the proteome.