In Vitro Mutagenesis Protocols

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In Vitro Mutagenesis Protocols

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Author : Jeff Braman
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05

In Vitro Mutagenesis Protocols written by Jeff Braman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.

Hands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications that allow high throughput mutagenesis experiments, and mutagenesis based on gene disruption (both in vitro- and in situ-based). Additional methods are provided for in vitro gene evolution; for gene disruption based on recombination, transposon, and casette mutagenesis; and for facilitating the introduction of multiple mutations. Time-tested and highly practical, the protocols in In Vitro Mutagenesis Protocols, 2nd Edition offer today's molecular biologists reliable and powerful techniques with which to illuminate the proteome.

In Vitro Mutagenesis Protocols

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Author : Jeff Braman
language : en
Publisher: Humana
Release Date : 2016-08-23

In Vitro Mutagenesis Protocols written by Jeff Braman and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.

In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications.

Gene Cloning And Analysis

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Author : B.C. Schaefer
language : en
Publisher: Taylor & Francis
Release Date : 2023-04-28

Gene Cloning And Analysis written by B.C. Schaefer and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-04-28 with Science categories.

This volume focuses on newly emerging technologies that facilitate the isolation and characterization of genes. The detailed protocols will be useful to the seasoned professional and easily understood by the novice. The vast majority of methods are applic

Mutation Breeding For Sustainable Food Production And Climate Resilience

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Author : Suprasanna Penna
language : en
Publisher: Springer Nature
Release Date : 2023-04-04

Mutation Breeding For Sustainable Food Production And Climate Resilience written by Suprasanna Penna and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-04-04 with Technology & Engineering categories.

This book highlights the recent progress on the applications of mutation breeding technology in crop plants. Plant breeders and agriculturists are faced with the new challenges of climate change, human population growth, and dwindling arable land and water resources which threaten to sustain food production worldwide. Genetic variation is the basis which plant breeders require to produce new and improved cultivars. The understanding of mutation induction and exploring its applications has paved the way for enhancing genetic variability for various plant and agronomic characters, and led to advances in gene discovery for various traits. Induced mutagenesis has played a significant role in crop improvement and currently, the technology has resulted in the development and release of more than 3600 mutant varieties in most of the crop plants with great economic impact. The field of ‘mutation breeding’ has come long way to become an important approach for crop improvement. This book covers various methodologies of mutation induction, screening of mutants, genome editing and genomics advances and mutant gene discovery. The book further discusses success stories in different countries and applications of mutation breeding in food crops, horticultural plants and plantation crops. This informative book is very useful to plant breeders, students and researchers in the field of agriculture, plant sciences, food science and genetics.

Protocols For Micropropagation Of Woody Trees And Fruits

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Author : S.Mohan Jain
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-09-18

Protocols For Micropropagation Of Woody Trees And Fruits written by S.Mohan Jain and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-09-18 with Technology & Engineering categories.

Micropropagation has become a reliable and routine approach for large-scale rapid plant multiplication, which is based on plant cell, tissue and organ culture on well defined tissue culture media under aseptic conditions. A lot of research efforts are being made to develop and refine micropropagation methods and culture media for large-scale plant multiplication of several number of plant species. However, many forest and fruit tree species still remain recalcitrant to in vitro culture and require highly specific culture conditions for plant growth and development. The recent challenges on plant cell cycle regulation and the presented potential molecular mechanisms of recalcitrance are providing excellent background for understanding on totipotency and what is more development of micropropagation protocols. For large-scale in vitro plant production the important attributes are the quality, cost effectiveness, maintenance of genetic fidelity, and long-term storage. The need for appropriate in vitro plant regeneration methods for woody plants, including both forest and fruit trees, is still overwhelming in order to overcome problems facing micropropagation such as somaclonal variation, recalcitrant rooting, hyperhydricity, polyphenols, loss of material during hardening and quality of plant material. Moreover, micropropagation may be utilized, in basic research, in production of virus-free planting material, cryopreservation of endangered and elite woody species, applications in tree breeding and reforestation.

Artificial Dna

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Author : Yury E. Khudyakov
language : en
Publisher: CRC Press
Release Date : 2002-09-25

Artificial Dna written by Yury E. Khudyakov and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-09-25 with Medical categories.

Combining elements of biochemistry, molecular biology, and immunology, artificial DNA can be employed in a number of scientific disciplines. Some of the varied applications include site-specific mutagenesis, hybridization, amplification, protein engineering, anti-sense technology, DNA vaccines, protein vaccines, recombinant antibodies, screening fo

Poxviruses

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Author : Richard W. Moyer
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Poxviruses written by Richard W. Moyer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

The purpose of this volume is to highlight some current areas of poxvirus research which are likely to be particularly fruitful in the upcoming few years. The first chapter, by Drs. Condit and Niles, discusses poxvirus genetics. Work in this area has provided mutants, produced practical procedures to simplify the manipulation of viral genes, and generated information about the molecular architecture and organization of genes characteristic of pox viruses. One of the most intensively studied regions of the viral genome is the HindIII D region of vaccinia, in which a combination of classical and molecular genetic analysis of the region has been particularly revealing. Within this region are open reading frames, some of which are expressed early and others late, organized in a fashion which is now known to be typical of these viruses. Other studies, related to temperature sensitive, drug resistant, and drug dependent mutants, are also discussed. Each of the other reviews included in this volume summarizes areas of research which have depended heavily on the genetics of the system. The intracellular site of a poxvirus infection is mostly, if not exclusively, limited to the cytoplasm which dictates several interesting biological ramifications. For example, poxvirus transcription must occur in the cytoplasm, rather than in the nucleus. The virus copes with this situation by incorporating into the virion the enzymatic machinery necessary to initiate transcription from input virus.

In Vitro Mutagenesis

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Author : Andrew Reeves
language : en
Publisher:
Release Date : 2016

In Vitro Mutagenesis written by Andrew Reeves and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with Biomedicine categories.

Annotation In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such methods as genome editing, transposon (Tn) mutagenesis, site-directed, and random mutagenesis. In Vitro Mutagenesis: Methods and Protocols guides readers through methods for gene and genome editing, practical bioinformatics approaches for identifying mutagenesis targets, and novel site-directed and random mutagenesis approaches aimed at gaining a better understanding of protein-protein and protein-cofactor interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, In Vitro Mutagenesis: Methods and Protocols aims to provide a highly accessible and practical manual for current and future molecular biology researchers, from the beginner practitioner to the advanced investigator in fields such as molecular genetics, biochemistry, and biochemical and metabolic engineering.

Pcr Protocols

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Author : Bruce A. White
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02

Pcr Protocols written by Bruce A. White and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.

PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.

Pcr In Bioanalysis

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Author : Stephen J Meltzer
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03

Pcr In Bioanalysis written by Stephen J Meltzer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.

PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.