Pcr In Bioanalysis
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Pcr In Bioanalysis
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Author : Stephen J Meltzer
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Pcr In Bioanalysis written by Stephen J Meltzer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.
Bioanalytics
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Author : Friedrich Lottspeich
language : en
Publisher: John Wiley & Sons
Release Date : 2018-05-29
Bioanalytics written by Friedrich Lottspeich and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-29 with Science categories.
Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology
Gene Cloning And Analysis By Rt Pcr
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Author : Paul D. Siebert
language : en
Publisher: Eaton Publishing Company/Biotechniques Books
Release Date : 1998
Gene Cloning And Analysis By Rt Pcr written by Paul D. Siebert and has been published by Eaton Publishing Company/Biotechniques Books this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Medical categories.
Photoproteins In Bioanalysis
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Author : Sylvia Daunert
language : en
Publisher: John Wiley & Sons
Release Date : 2006-10-06
Photoproteins In Bioanalysis written by Sylvia Daunert and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-10-06 with Science categories.
The use of light-emitting proteins for the detection of biomolecules provides fast and sensitive methods which overcome the disadvantages of radioactive labels and the high cost of fluorescent dyes. This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to name just a few, and is complemented by recent advances in instrumentation. Includes an introductory chapter by 2008 Chemistry Nobel laureate Osamu Shimomura.
Drug Development For Gene Therapy
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Author : Yanmei Lu
language : en
Publisher: John Wiley & Sons
Release Date : 2024-02-09
Drug Development For Gene Therapy written by Yanmei Lu and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-02-09 with Medical categories.
Drug Development for Gene Therapy Industry-centric perspective on translational and bioanalytical challenges and best practices for gene therapies Drug Development for Gene Therapy focuses on the translational and bioanalytical challenges and best practices for gene therapy modalities, presenting a significant body of data, including information related to safety and efficacy, necessary to advance through the development pipeline into clinical use. The text covers bioanalytical methods and platforms including patient screening assays, different PCR tests, enzyme activity assays, ELISpot, NGS, LC/MS, and immunoassays, with FDA and EMA guidelines on gene therapy safety and efficacy, along with companion diagnostics regulations from US and EU perspectives. The chapters offer an in-depth discussion of the basics and best practices for translational biomarkers, bioanalysis, and developing companion diagnostics / lab tests for gene therapies in the pharma and biopharma industries. To aid in reader comprehension, the text includes clinical examples of relevant therapies in related chapters. Some of the core topics covered include study design, immunogenicity, various bioanalytical methods and their applications, and global regulatory issues. Written by two highly qualified authors with significant experience in the field, Drug Development for Gene Therapy includes information on: Bioanalytical methods to detect pre-existing antibodies against adeno-associated viruses (AAV) capsids Detection of cellular immunity and humoral response to viral capsids and transgene proteins, and immunogenicity of gene therapy products Nonclinical and clinical study considerations and methods for biodistribution and shedding Quantification of transgene protein expression and biochemical function, and substrate and distal pharmacodynamic biomarker measurements for gene therapy Detection and quantification of rAAV integration and off-target editing Current regulatory landscape for gene therapy product development and the role of biomarkers and general regulatory considerations for gene therapy companion diagnostics With comprehensive coverage of the subject, Drug Development for Gene Therapy is a must-have resource for researchers and developers in the areas of pharmaceuticals, biopharmaceuticals, and contract research organizations (CROs), along with professors, researchers, and advanced students in chemistry, biological, biomedical engineering, pharmaceuticals, and medical sciences.
An Introduction To Bioanalysis Of Biopharmaceuticals
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Author : Seema Kumar
language : en
Publisher: Springer Nature
Release Date : 2022-08-23
An Introduction To Bioanalysis Of Biopharmaceuticals written by Seema Kumar and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-08-23 with Science categories.
The book provides a comprehensive review of the fundamental and practical aspects of bioanalytical support and the integral role it plays in the development of safe and efficacious biopharmaceutical drugs with speed and cost-effectiveness. The book focuses on a broad range of conventional and emerging biopharmaceutical modalities including monoclonal antibody-based therapeutics, gene therapy, cell therapy, peptides and oligonucleotides. The book starts with an introductory overview of bioanalysis showcasing the integral role it plays in understanding the drug disposition (pharmacokinetics/pharmacodynamics and immunogenicity) and the progression of bioanalytical strategy as the drug progresses through discovery and development stages of the program, taking into consideration the continually evolving regulatory landscape. The book further diversifies into individual biopharmaceutical modalities - monoclonal antibodies, antibody-drug conjugates, bispecifics, Fc-fusion proteins, gene therapies, cell therapies, peptides and oligonucleotides. The individual chapters focus on modality-specific bioanalytical assay strategies, critical reagents, assay formats, analytical platforms, associated bioanalytical challenges and mitigation strategies, industry best practices, and the latest understanding of regulatory guidance as applicable to the fast-growing biopharmaceutical landscape.
Bioanalytical Chemistry
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Author : Paolo Ugo
language : en
Publisher: Walter de Gruyter GmbH & Co KG
Release Date : 2021-02-08
Bioanalytical Chemistry written by Paolo Ugo and has been published by Walter de Gruyter GmbH & Co KG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-02-08 with Science categories.
The urgent need for rapid and reliable analytical tools suitable to perform a large number of high quality analyses of biological molecules has been dramatically stressed by the recent crisis caused by the COVID-19 pandemic. This book provides graduate students and young researchers with the elements of interdisciplinary knowledge necessary to apply the wide arsenal of bioanalytical devices and methods available today.
Bioanalytical Chemistry
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Author : Paolo Ugo
language : en
Publisher: Walter de Gruyter GmbH & Co KG
Release Date : 2021-02-08
Bioanalytical Chemistry written by Paolo Ugo and has been published by Walter de Gruyter GmbH & Co KG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-02-08 with Technology & Engineering categories.
Bioanalytical chemistry plays today a central role in various fields, from healthcare to food and environmental control. This book presents the main methodologies for analyzing biomacromolecules, with a focus on methods based on molecular recognition. The six chapters move from fundamentals to the most recent advances, achieved by a synergetic combination of bio and nanotechnologies. The need for rapid and reliable analytical tools able to perform a large number of quantitative analyses, not only in centralized laboratories and core facilities but also for point-of-care testing, has been dramatically stressed by the recent crisis caused by the COVID-19 pandemic. The aim of the authors is to provide graduate students and young researchers with the elements of interdisciplinary knowledge necessary not only to use the wide arsenal of bioanalytical tools available today but also to contribute to the development of even more effective devices and methods.
Stress Response
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Author : Stephen M. Keyse
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-26
Stress Response written by Stephen M. Keyse and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-26 with Science categories.
Mammalian cells have evolved a complex multicomponent machinery that enables them to sense and respond to a wide variety of potentially toxic agents present in their environment. These stress responses are often associated with an increased cellular capacity to tolerate normally lethal levels of an insult. The realization that the mammalian stress response may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer, has led to an explosion of interest in this research area. Stress Response: Methods and Protocols brings together a diverse array of practical methodologies that may be employed to address various aspects of the response of mammalian cells to environmental stress. The p- tocols are carefully described by authors who have both devised and succe- fully employed them, and they represent a mixture not only of well-established techniques, but also new technologies at the leading edge of research. The areas covered include the detection and assay of stress-induced damage, the acti- tion of signal transduction pathways, stress-inducible gene expression, and stress protein function. Although no volume of this size can be comprehensive and the topics covered reflect a personal choice, it is hoped that it will prove of subst- tial interest and use to a wide range of research workers in the field.
Aptamers In Bioanalysis
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Author : M. Mascini
language : en
Publisher: John Wiley & Sons
Release Date : 2009-04-27
Aptamers In Bioanalysis written by M. Mascini and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-04-27 with Science categories.
This is the first book to detail bioanalytical technologies and methods that have been developed using aptamers in analytical, medical, environmental, and food science applications. After an introduction to aptamers, aptamer targets, and their general uses, it discusses different applications with particular attention to the comparison between aptamer-based biosensors and methods versus the corresponding immunosensors. Examples of aptamer-based diagnostic techniques include whole-cell protein profiling (proteomics) and medical diagnostics for the distinction of diseased versus healthy states. This is a core reference for analytical chemists, electrochemists, pharmaceutical/medicinal chemists, biotechnologists, and others.