Insect Cells

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Insect Cell Cultures

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Author : Just M. Vlak
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-04-11

Insect Cell Cultures written by Just M. Vlak and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-04-11 with Science categories.

A comprehensive reference work covering the key issues in insect cell cultures, this text includes 30 review papers on such topics as: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; and economics and regulatory aspects.; This text should be useful for cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.

Insect Cell Culture Engineering

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Author : Mattheus F. A. Goosen
language : en
Publisher: CRC Press
Release Date : 2020-07-24

Insect Cell Culture Engineering written by Mattheus F. A. Goosen and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-24 with Science categories.

Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modelling in animal cell culture; addresses the concerns associated with insect cell immobilization and the use of serum-free culture media; provides insights into the protective effects of polymer additives and insect cell gene expression in pharmaceutical research; and analyzes process scale-up and reactor design.;Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines.

Insect Cells

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Author : David Spencer Smith
language : en
Publisher:
Release Date : 1968

Insect Cells written by David Spencer Smith and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1968 with Cells categories.

Insect Cell Biotechnology

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Author : Gordon D. O. Maramorosch
language : en
Publisher: CRC Press
Release Date : 2018-01-18

Insect Cell Biotechnology written by Gordon D. O. Maramorosch and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-18 with Science categories.

Insect Cell Biotechnology provides a lucid, up-to-date description of recent major advances in the field. A number of significant topics are addressed, including the use and production of baculoviruses in insect cells, baculovirus specificity, bacterial toxin studies in cultured insect cells, scale-up operations required in the production of recombinant protein and insect viruses propagated in insect cells, growth and nervous system interactions, and the physiological and developmental capacities of cell lines. Transfection in Drosophila cells and a chapter on the theoretical and practical implications of stress produced by x-rays, ultraviolet light, chemicals, psoralens, and heat are discussed as well.

The Ultrastructure And Functioning Of Insect Cells

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Author : Hiromu Akai
language : en
Publisher:
Release Date : 1982

The Ultrastructure And Functioning Of Insect Cells written by Hiromu Akai and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1982 with Entomology categories.

Baculovirus And Insect Cell Expression Protocols

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Author : David W. Murhammer
language : en
Publisher: Methods in Molecular Biology
Release Date : 2018-04-15

Baculovirus And Insect Cell Expression Protocols written by David W. Murhammer and has been published by Methods in Molecular Biology this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-15 with Medical categories.

The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.

Baculovirus Expression Protocols

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Author : Christopher D. Richardson
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02

Baculovirus Expression Protocols written by Christopher D. Richardson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.

Baculovirus Expression Protocols offers both industrial and university-based researchers a comprehensive comilation of the latest baculovirus techniques along with step-by-step instructions and time-saving techniques. The contributors-leading authorities in the field-present the assorted expression plasmids currently in use, guide the reader through the process of generating and selecting recombinant virus, and describe specific examples of recombinant protein production and purification. The emphasis is on alternative and simpler screening techniques for the selection of recombinant baculovirus. The book also surveys the various insect cell lines currently compatible with the baculovirus system. Highlights include production of recombinant virus using linearized DNA and vectors that contain a b-galactosidase indicator and a complete list of expression vectors currently available. Detailed descriptions for the scale-up of protein production using spinner flasks, bioreactors, and insect larvae are also included. Baculovirus Expression Protocols offers both industrial and university-based researchers an outstanding collection of reproducible, step-by-step laboratory protocols. It will immediately become indispensable for anyone working with baculoviruses and their application in the expression of recombinant proteins in insect cells.

Insect Cell Biotechnology

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Author : Karl Maramorosch
language : en
Publisher: CRC Press
Release Date : 1994-05-27

Insect Cell Biotechnology written by Karl Maramorosch and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-05-27 with Medical categories.

Invertebrate Cell System Applications

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Author : Jun Mitsuhashi
language : en
Publisher: CRC Press
Release Date : 2018-01-18

Invertebrate Cell System Applications written by Jun Mitsuhashi and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-18 with Science categories.

A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.

Insect Ultrastructure

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Author : H. Akai
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-09

Insect Ultrastructure written by H. Akai and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-09 with Science categories.

Fourteen years have passed since the publication of David Spencer Smith's Insect Cells: Their Structure and Function. Here the results of a decade of electronmicroscopic studies on insect cells were summarized in an organized and integrated fashion for the first time, and the ultrastructural characteristics of different specialized cells and tissues were abundantly illustrated in the 117 plates this monograph contained. In the intervening period great progress has been made in the field of Insect Ultrastructure. Organelles not even mentioned in Smith's book, such as synaptonemal complexes, clathrin baskets, fusomes, and retinular junc tions, have been identified and functions proposed for them. There have also been many technical advances that have profoundly influenced the direction of subsequent research. A spectacular example would be the development by Miller and Beatty of the chromosomal spreading technique which allowed for the first time ultrastructural studies on segments of chromosomes containing genes in various stages of replication and transcription. Then there is the freeze-fracture procedure first described by Moor and his colleagues. This technique permitted an analysis of intercellular junctions that was impossible with the conventional sectioning methods. The results greatly clarified our understanding of the channels for ion movement and the permeability barriers between cells and also the membrane changes that occur during the embryonic differentiation and metamorphosis of various types of insect cells.

Insect Cells

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