Insertional Mutagenesis Strategies In Cancer Genetics
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Insertional Mutagenesis Strategies In Cancer Genetics
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Author : Adam J. Dupuy
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-11-18
Insertional Mutagenesis Strategies In Cancer Genetics written by Adam J. Dupuy and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-18 with Medical categories.
The goal of this work is summarize the contribution that insertional mutagenesis has made to our understanding of cancer. A variety of insertional mutagens are presented that have been used to study a variety of tumor types in several model organisms. In addition, the impact of insertional mutagenesis in several gene therapy trials is discussed along with strategies to avoid such complications in future clinical trials.
Insertional Mutagenesis As A Tool For Identifying And Characterizing Novel Candidate Cancer Genes
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Author : Jacquelyn Jinan Roth
language : en
Publisher:
Release Date : 2011
Insertional Mutagenesis As A Tool For Identifying And Characterizing Novel Candidate Cancer Genes written by Jacquelyn Jinan Roth and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.
Cancer results from dynamic changes in a set of cellular genes. Mutations in oncogenes and tumor suppressor genes are responsible for converting a normal cell into a malignant one. Much research has been done to identify a large number of oncogenes and tumor suppressor genes that are frequently mutated in human cancers. These efforts suggest that the cancer genome is composed of a few commonly mutated genes along with hundreds of infrequently mutated genes. Deciphering which of the mutations are important in tumorigenesis will aid in understanding the biology of cancer as well as provide potential new therapeutic targets. Mouse models of human cancer have been used to determine the significance genetic alterations identified in human cancers. One tool that has been used for this purpose in the mouse is insertional mutagenesis. In our study, we designed a Sleeping Beauty transposon-based forward genetic screen in mice to identify candidate genes for breast cancer in the presence and absence of Cav1 mutations. Analysis of transposon integrations in all mammary tumors identified 210 common insertion sites (CISs) and candidate (CAN) genes were assigned to many of the CISs. Multiple CAN genes have been previously implicated in human breast cancers. Identifying the roles of these 210 CAN genes in tumorigenesis will determine their significance in human breast cancer. In this study, we also investigated the role of a transcription factor, Meis1, which was originally identified in murine leukemias caused by retroviral-induced insertional mutagenesis. Overexpression of Meis1 in conjunction with HoxA9 is detected in a variety of myeloid leukemia cell lines and primary human samples of acute myeloid leukemia (AML). In an effort to identify additional downstream targets of Meis1, we generated FDC-P1 cells overexpressing Meis1 in the presence or absence of HoxA9. Microarray analysis was performed on RNA isolated from these cells. A combination of bioinformatics and statistical analyses were used to look for genes with differential regulation in the presence or absence of Meis1 and HoxA9. These data were used to generate a genetic signature characteristic of Meis1 expression and shed light onto other pathways that may involve Meis1 and HoxA9.
Identification Of Tumor Suppressor Genes In Breast Cancer By Insertional Mutagenesis And Functional Inactivation 96 Breast
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Author :
language : en
Publisher:
Release Date : 1998
Identification Of Tumor Suppressor Genes In Breast Cancer By Insertional Mutagenesis And Functional Inactivation 96 Breast written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with categories.
The development and progression of cancer result from multiple genetic changes accumulated in the cells. The identification of tumor suppressor genes inactivated and proto-oncogenes activated in mammary epithelial cells is essential to understand the genetic basis of breast cancer and is a prerequisite for development of strategies for prevention, diagnosis, and treatment. In breast cancer, loss of heterozygosity (LOR) was detected frequently on chromosome 17 and other chromosomes, suggesting unrecognized tumor suppressor genes. We are applying the novel retroviral-tagging strategy to identify the genes using chromosome 1 7-suppressed (independent of p53 and ERCAl) breast cancer cell lines. In contrast to the parental tumorigenic cell line CAL51, the suppressed sublines CAL/17-1, CAL/17-3 and CAL/17-5 display insulin-dependent growth in flasks, no growth in soft-agar culture and athymic nude mice. In this annual report, we present our results on selection for the anchorage-independent cell sublines induced by retroviral transduction of the chromosome 17-mediated suppressed cell lines CAL/ 17, in addition to successful selection for insulin-independent cell sublines. We are now in the process to conduct tumorigenicity tests in athymic nude mice and to clone genomic sequences flanking the integrated retroviral vectors.
Finding Cancer Genes In Copy Number Data And Insertional Mutagenesis Data
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Author : Christiaaan Nicolaas Klijn
language : en
Publisher:
Release Date : 2011
Finding Cancer Genes In Copy Number Data And Insertional Mutagenesis Data written by Christiaaan Nicolaas Klijn and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.
Transposon Mediated Insertional Mutagenesis In Gene Discovery And Cancer
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Author : Jun Kong
language : en
Publisher:
Release Date : 2011
Transposon Mediated Insertional Mutagenesis In Gene Discovery And Cancer written by Jun Kong and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.
Gene Therapy For Cancer
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Author : Kelly K. Hunt
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-26
Gene Therapy For Cancer written by Kelly K. Hunt and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-26 with Medical categories.
The three sections of this volume present currently available cancer gene therapy techniques. Part I describes the various aspects of gene delivery. In Part II, the contributors discuss strategies and targets for the treatment of cancer. Finally, in Part III, experts discuss the difficulties inherent in bringing gene therapy treatment for cancer to the clinic. This book will prove valuable as the volume of preclinical and clinical data continues to increase.
Holland Frei Cancer Medicine
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Author : Robert C. Bast, Jr.
language : en
Publisher: John Wiley & Sons
Release Date : 2017-03-10
Holland Frei Cancer Medicine written by Robert C. Bast, Jr. and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-03-10 with Medical categories.
Holland-Frei Cancer Medicine, Ninth Edition, offers a balanced view of the most current knowledge of cancer science and clinical oncology practice. This all-new edition is the consummate reference source for medical oncologists, radiation oncologists, internists, surgical oncologists, and others who treat cancer patients. A translational perspective throughout, integrating cancer biology with cancer management providing an in depth understanding of the disease An emphasis on multidisciplinary, research-driven patient care to improve outcomes and optimal use of all appropriate therapies Cutting-edge coverage of personalized cancer care, including molecular diagnostics and therapeutics Concise, readable, clinically relevant text with algorithms, guidelines and insight into the use of both conventional and novel drugs Includes free access to the Wiley Digital Edition providing search across the book, the full reference list with web links, illustrations and photographs, and post-publication updates
Cancer Gene Therapy
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Author : David T. Curiel
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-11-03
Cancer Gene Therapy written by David T. Curiel and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-03 with Medical categories.
A complete introduction and guide to the latest developments in cancer gene therapy-from bench to bedside. The authors comprehensively review the anticancer genes and gene delivery methods currently available for cancer gene therapy, including the transfer of genetic material into the cancer cells, stimulation of the immune system to recognize and eliminate cancer cells, and the targeting of the nonmalignant stromal cells that support their growth. They also thoroughly examine the advantages and limitations of the different therapies and detail strategies to overcome obstacles to their clinical implementation. Topics of special interest include vector-targeting techniques, the lessons learned to date from clinical trials of cancer gene therapy, and the regulatory guidelines for future trials. Noninvasive techniques to monitor the extent of gene transfer and disease regression during the course of treatment are also discussed.
Mammography And Beyond
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Author : National Research Council
language : en
Publisher: National Academies Press
Release Date : 2001-06-04
Mammography And Beyond written by National Research Council and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-06-04 with Medical categories.
X-ray mammography screening is the current mainstay for early breast cancer detection. It has been proven to detect breast cancer at an earlier stage and to reduce the number of women dying from the disease. However, it has a number of limitations. These current limitations in early breast cancer detection technology are driving a surge of new technological developments, from modifications of x-ray mammography such as computer programs that can indicate suspicious areas, to newer methods of detection such as magnetic resonance imaging (MRI) or biochemical tests on breast fluids. To explore the merits and drawbacks of these new breast cancer detection techniques, the Institute of Medicine of the National Academy of Sciences convened a committee of experts. During its year of operation, the committee examined the peer-reviewed literature, consulted with other experts in the field, and held two public workshops. In addition to identifying promising new technologies for early detection, the committee explored potential barriers that might prevent the development of new detection methods and their common usage. Such barriers could include lack of funding from agencies that support research and lack of investment in the commercial sector; complicated, inconsistent, or unpredictable federal regulations; inadequate insurance reimbursement; and limited access to or unacceptability of breast cancer detection technology for women and their doctors. Based on the findings of their study, the committee prepared a report entitled Mammography and Beyond: Developing Technology for Early Detection of Breast Cancer, which was published in the spring of 2001. This is a non-technical summary of that report.
Pharmaceutical Biotechnology
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Author : Daan J. A. Crommelin
language : en
Publisher: CRC Press
Release Date : 2002-11-14
Pharmaceutical Biotechnology written by Daan J. A. Crommelin and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-11-14 with Medical categories.
The field of pharmaceutical biotechnology is evolving rapidly. A whole new arsenal of protein pharmaceuticals is being produced by recombinant techniques for cancer, viral infections, cardiovascular and hereditary disorders, and other diseases. In addition, scientists are confronted with new technologies such as polymerase chain reactions, combinatorial chemistry and gene therapy. This introductory textbook provides extensive coverage of both the basic science and the applications of biotechnology-produced pharmaceuticals, with special emphasis on their clinical use. Pharmaceutical Biotechnology serves as a complete one-stop source for undergraduate pharmacists, and it is valuable for researchers and professionals in the pharmaceutical industry as well.