Insilico Identification And Optimization Of Natural Inhibitors For Drug Target Sites In Cryptosporidium Parvum
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Insilico Identification And Optimization Of Natural Inhibitors For Drug Target Sites In Cryptosporidium Parvum
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Author : Dr. Pratibha Teotia
language : en
Publisher: Manojvm Publishing House
Release Date :
Insilico Identification And Optimization Of Natural Inhibitors For Drug Target Sites In Cryptosporidium Parvum written by Dr. Pratibha Teotia and has been published by Manojvm Publishing House this book supported file pdf, txt, epub, kindle and other format this book has been release on with Fiction categories.
Now day’s computer-aided drug design considered as a powerful method to design very specific lead compounds that can be developed as drug molecules. Using different in-silico tools, a target is selected and then its structure is defined and determined. After that new chemical/ synthetic compounds can be designed in-silico on the basis of combinatorial chemistry or chosen from an already available chemical library of molecules or library of molecules is generated from a subset of small molecules on the basis of docking and scoring against the particular target. In this study, I attempt to generate 2D QSAR model using small pIC50 values for thirty-eight benzoxazole derivatives binding with C. parvum IMPDH protein resulting correlation coefficient value R2/r2 is 0.7948. Docking results show that out of 38 benzoxazole derivatives, four compounds are most active. The present examination may give the data about potential derivatives of Benzoxazole as chemotherapeutic operators to battle against the expanding weight of Cryptosporidiosis infections.
In Silico Drug Design
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Author : Kunal Roy
language : en
Publisher: Academic Press
Release Date : 2019-02-12
In Silico Drug Design written by Kunal Roy and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-02-12 with Medical categories.
In Silico Drug Design: Repurposing Techniques and Methodologies explores the application of computational tools that can be utilized for this approach. The book covers theoretical background and methodologies of chem-bioinformatic techniques and network modeling and discusses the various applied strategies to systematically retrieve, integrate and analyze datasets from diverse sources. Other topics include in silico drug design methods, computational workflows for drug repurposing, and network-based in silico screening for drug efficacy. With contributions from experts in the field and the inclusion of practical case studies, this book gives scientists, researchers and R&D professionals in the pharmaceutical industry valuable insights into drug design. - Discusses the theoretical background and methodologies of useful techniques of cheminformatics and bioinformatics that can be applied for drug repurposing - Offers case studies relating to the in silico modeling of FDA-approved drugs for the discovery of antifungal, anticancer, antiplatelet agents, and for drug therapies against diseases - Covers tools and databases that can be utilized to facilitate in silico methods for drug repurposing
Molecular Diagnostics Promises And Possibilities
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Author : Mousumi Debnath
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-01-29
Molecular Diagnostics Promises And Possibilities written by Mousumi Debnath and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-01-29 with Medical categories.
A rapid development in diverse areas of molecular biology and genetic engineering resulted in emergence of variety of tools. These tools are not only applicable to basic researches being carried out world over, but also exploited for precise detection of abnormal conditions in plants, animals and human body. Although a basic researcher is well versed with few techniques used by him/her in the laboratory, they may not be well acquainted with methodologies, which can be used to work out some of their own research problems. The picture is more blurred when the molecular diagnostic tools are to be used by physicians, scientists and technicians working in diagnostic laboratories in hospitals, industry and academic institutions. Since many of them are not trained in basics of these methods, they come across several gray areas in understanding of these tools. The accurate application of molecular diagnostic tools demands in depth understanding of the methodology for precise detection of the abnormal condition of living body. To meet the requirements of a good book on molecular diagnostics of students, physicians, scientists working in agricultural, veterinary, medical and pharmaceutical sciences, it needs to expose the reader lucidly to: Give basic science behind commonly used tools in diagnostics Expose the readers to detailed applications of these tools and Make them aware the availability of such diagnostic tools The book will attract additional audience of pathologists, medical microbiologists, pharmaceutical sciences, agricultural scientists and veterinary doctors if the following topics are incorporated at appropriate places in Unit II or separately as a part of Unit-III in the book. Molecular diagnosis of diseases in agricultural crops Molecular diagnosis of veterinary diseases. Molecular epidemiology, which helps to differentiate various epidemic strains and sources of disease outbreaks. Even in different units of the same hospital, the infections could be by different strains of the same species and the information becomes valuable for infection control strategies. Drug resistance is a growing problem for bacterial, fungal and parasitic microbes and the molecular biology tools can help to detect the drug resistance genes without the cultivation and in vitro sensitivity testing. Molecular diagnostics offers faster help in the selection of the proper antibiotic for the treatment of tuberculosis, which is a major problem of the in the developing world. The conventional culture and drug sensitivity testing of tuberculosis bacilli is laborious and time consuming, whereas molecular diagnosis offers rapid drug resistant gene detection even from direct clinical samples. The same approach for HIV, malaria and many more diseases needs to be considered. Molecular diagnostics in the detection of diseases during foetal life is an upcoming area in the foetal medicine in case of genetic abnormalities and infectious like TORCH complex etc. The book will be equally useful to students, scientists and professionals working in the field of molecular diagnostics.
Alternative Respiratory Pathways In Higher Plants
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Author : Kapuganti Jagadis Gupta
language : en
Publisher: John Wiley & Sons
Release Date : 2015-06-15
Alternative Respiratory Pathways In Higher Plants written by Kapuganti Jagadis Gupta and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-06-15 with Science categories.
Rapid developments in molecular and systems biology techniques have allowed researchers to unravel many new mechanisms through which plant cells switch over to alternative respiratory pathways. This book is a unique compendium of how and why higher plants evolved alternative respiratory metabolism. It offers a comprehensive review of current research in the biochemistry, physiology, classification and regulation of plant alternative respiratory pathways, from alternative oxidase diversity to functional marker development. The resource provides a broad range of perspectives on the applications of plant respiratory physiology, and suggests brand new areas of research. Other key features: written by an international team of reputed plant physiologists, known for their pioneering contributions to the knowledge of regular and alternative respiratory metabolism in higher plants includes step-by-step protocols for key molecular and imaging techniques advises on regulatory options for managing crop yields, food quality and environment for crop improvement and enhanced food security covers special pathways which are of key relevance in agriculture, particularly in plant post-harvest commodities Primarily for plant physiologists and plant biologists, this authoritative compendium will also be of great value to postdoctoral researchers working on plant respiration, as well as to graduate and postgraduate students and university staff in Plant Science. It is a useful resource for corporate and private firms involved in developing functional markers for breeding programs and controlling respiration for the prevention of post-harvest losses in fruit, vegetables, cut flowers and tubers.
World Malaria Report 2019
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Author : World Health Organization
language : en
Publisher:
Release Date : 2019-08-28
World Malaria Report 2019 written by World Health Organization and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-28 with categories.
The World Malaria Report 2019 provides a comprehensive update on global and regional malaria data and trends. The report tracks investments in malaria programs and research as well as progress across all intervention areas: prevention, diagnosis, treatment, elimination, and surveillance. It also includes dedicated chapters on the consequences of malaria on maternal infant and child health the "High Burden to High Impact" approach as well as biological threats to the fight against malaria. The 2019 report is based on information received from more than 80 countries and areas with ongoing malaria transmission. This information is supplemented by data from national household surveys and databases held by other organizations.
Antiviral Drug Discovery And Development
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Author : Xinyong Liu
language : en
Publisher: Springer Nature
Release Date : 2021-07-13
Antiviral Drug Discovery And Development written by Xinyong Liu and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-07-13 with Medical categories.
This book summarizes state-of-the-art antiviral drug design and discovery approaches starting from natural products to de novo design, and provides a timely update on recently approved antiviral drugs and compounds in advanced clinical development. Special attention is paid to viral infections with a high impact on the world population or highly relevant from the public health perspective (HIV, hepatitis C, influenza virus, etc.). In these chapters, limitations associated with adverse effects and emergence of drug resistance are discussed in detail. In addition to classical antiviral strategies, chapters will be dedicated to discuss the non-classical drug development strategies to block viral infection, for instance, allosteric inhibitors, covalent antiviral agents, or antiviral compounds targeting protein–protein interactions. Finally, current prospects for producing broad-spectrum antiviral inhibitors will be also addressed. The book is distinctive in providing the most recent update in the rapidly evolving field of antiviral therapeutics. Authoritative reviews are written by international scientists well known for their contributions in their topics of research, which makes this book suitable for researchers not only within the antiviral research community but also attractive to a broad audience in the drug discovery field. This book covers molecular structures and biochemical mechanisms mediating the antiviral effects, while discussing various ligand design strategies, which include traditional medicinal chemistry, computational chemistry, and chemical biology approaches. The book provides a comprehensive review of antiviral drug discovery and development approaches, particularly focusing on current innovations and future trends.
Parasitic Protozoa Of Farm Animals And Pets
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Author : Monica Florin-Christensen
language : en
Publisher: Springer
Release Date : 2018-04-21
Parasitic Protozoa Of Farm Animals And Pets written by Monica Florin-Christensen and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-21 with Medical categories.
This book provides an in-depth yet concise overview of the most common and emerging protozoa that cause diseases in both farm animals and companion animals. As outlined in the concise introduction, pathogenic protozoans represent an evolutionary highly diverse and little understood group of disease-causing microorganisms. For each of the featured parasitic unicellular eukaryotes, it discusses the morphology, lifecycle, epidemiology and host-pathogen interactions. In addition, the book highlights the latest developments in diagnostic methods, as well as prevention and treatment strategies. Thorough information on genomes and genetic manipulation strategies for some of the protozoa covered in this book is also included. Infections involving parasitic protozoa can cause productivity losses and/or reduce the quality of life of infected animals. Some infections are zoonotic, posing an on-going public health threat. In most cases, prevention and treatment are either non-existent or need considerable improvement. On the other hand, a great deal of research has recently been conducted on these organisms, yielding valuable new information on their global distribution and revealing the mechanisms of host-pathogen interactions at the molecular level – and essential insights that can be used for the development of new control tools. This book includes extensive information on both basic aspects and recent scientific discoveries on these protozoa and thus constitutes a unique resource for students, veterinarians, and researchers alike.
Real Time Pcr
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Author : M Tevfik Dorak
language : en
Publisher: Garland Science
Release Date : 2007-02-08
Real Time Pcr written by M Tevfik Dorak and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-02-08 with Science categories.
With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.
Molecular Diagnostics
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Author : Lela Buckingham
language : en
Publisher: F. A. Davis Company
Release Date : 2012
Molecular Diagnostics written by Lela Buckingham and has been published by F. A. Davis Company this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Medical categories.
The first text on molecular diagnostics specifically designed for clinical laboratory science programs is back! This exceptional resource introduces the fundamentals of nucleic acid, as well as more advanced concepts. With a focus on the application of molecular concepts in the clinical laboratory to diagnosis diseases, the 2nd Edition includes important updates and improvements to keep up with the rapidly developing field. Inside youll find in-depth explanations of the principles of molecular-based assays as well as reference material, trouble-shooting tips for the laboratory, and discussions that emphasize the continuing emergence of new diagnostic technologies.
Combinative Strategy To Advance Target Based Anticryptosporidial Drug Discovery
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Author : Jubilee Ajiboye
language : en
Publisher:
Release Date : 2025
Combinative Strategy To Advance Target Based Anticryptosporidial Drug Discovery written by Jubilee Ajiboye and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025 with CRISPR-associated protein 9 categories.
Cryptosporidiosis is a prevalent diarrheal disease characterized by infection of the small intestine by apicomplexan Cryptosporidium parasites. These intestinal parasites effectuate life-threatening diarrhea in young children and immunocompromised patients such as those on long-term immunosuppressants, or people living with HIV/AIDS. There are presently no vaccines to prevent cryptosporidiosis in humans. Unfortunately, the only FDA-approved treatment, nitazoxanide, lacks efficacy in immunocompromised patients but shows moderate efficacy in children, populations in which cryptosporidiosis is most severe and persistent. To engage the obvious paucity in the availability of novel anti-Cryptosporidium therapeutics, large-scale phenotypic screenings of compounds made available by Merck KGaA, Darmstadt, Germany, led to the identification of pyrazolopyrimidine human phosphodiesterase (PDE)-V (hsPDE-V) and 1,5-naphthyridine phosphatidylinositol-4 kinase (PI4K) inhibitors with potent in vitro anticryptosporidial characteristics and in vivo efficacy following oral administration in C. parvum-infected immunocompromised mouse models of cryptosporidiosis. The lead phosphodiesterase inhibitor (PDEi) and phosphatidylinositol-4-kinase inhibitor (PI4Ki) series showed comparable anti-C. parvum and C. hominis potency, are fast-acting in tissue culture, and have minimal off-target effects in a preliminary safety screening assay panel. Our main objective was to validate the molecular targets of the novel PDEis and PI4Kis lead series in Cryptosporidium. By extension, we sought to uncover the mode-of-action of these novel lead series in C. parvum and highlight important target-based strategies that can be exploited for drug target identification in anticryptosporidial drug discovery. We demonstrated the mode-of-action of the lead series by employing life-cycle phenotypic assays which identified the parasite egress stage as the key life stage blocked by the PDEi and PI4Ki series. Subsequently, we utilized in vitro enzyme assays to confirm on-target engagement of our lead PDEis and PI4Kis against recombinant CpPDE1/CpPDE3 and CpPI4K enzymes, respectively. Guided by in silico analyses, we identified two residues (Val900 and His884) in the CpPDE1 active site predicted to be important for pyrazolopyrimidine PDEi binding. We produced a CRISPR-engineered C. parvum CpPDE1-V900A transgenic strain which exhibited altered susceptibility to our lead PDEi series, providing genetic support for CpPDE1-pyrazolopyrimidine PDEi interaction. Our findings suggest that CpPDE1, a validated pyrazolopyrimidine molecular target, can be exploited for target-based lead optimization in our anticryptosporidial drug development scheme. Furthermore, to genetically validate CpPI4K as a molecular target of the novel naphthyridine PI4Ki lead series, we overexpressed the wild-type CpPI4K gene in wild-type C. parvum to confirm the development of a resistance phenotype in the CpPI4K over-expressing transgenic strain. The expression of an additional copy of the wild-type CpPI4K gene conferred a moderate resistance phenotype in the presence of a naphthyridine PI4Ki and a separate imidazopyrazine PI4Ki by about 3-fold. These results provide compelling evidence that CpPI4K is a molecular target of the imidazopyrazine and novel naphthyridine PI4Ki lead series. In summary, we have identified and validated CpPI4K and CpPDE1 as molecular targets of our PI4Ki and PDEi lead series, respectively. Our target identification efforts on CpPDE1 marks the first characterization of the CpPDE1 as a druggable target in C. parvum. Meanwhile, our genetic validation of CpPI4K druggability will build on existing research in the anti-Cryptosporidium drug discovery field. Collectively, the results from this work will inform medicinal chemistry lead optimization efforts to advance anticryptosporidial drug development.