Introduction To Confocal Fluorescence Microscopy


Introduction To Confocal Fluorescence Microscopy
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Introduction To Confocal Fluorescence Microscopy


Introduction To Confocal Fluorescence Microscopy
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Author : Michiel Muller
language : en
Publisher: SPIE Press
Release Date : 2006

Introduction To Confocal Fluorescence Microscopy written by Michiel Muller and has been published by SPIE Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Science categories.


This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data. The appendices provide a quick reference to optical theory, microscopy-related formulas and definitions, and Fourier theory.



Introduction To Confocal Fluorescence Microscopy


Introduction To Confocal Fluorescence Microscopy
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Author : Michiel Müller
language : en
Publisher:
Release Date : 2002

Introduction To Confocal Fluorescence Microscopy written by Michiel Müller and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Confocal microscopy categories.




Principles Of Three Dimensional Imaging In Confocal Microscopes


Principles Of Three Dimensional Imaging In Confocal Microscopes
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Author : Min Gu
language : en
Publisher: World Scientific
Release Date : 1996

Principles Of Three Dimensional Imaging In Confocal Microscopes written by Min Gu and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with Science categories.


This book discusses the various principles in confocal scanning microscopy which has become a useful tool in many practical fields including biological studies and industrial inspection. The methodology presented in this book is unique and is based on the concept of the three-dimensional transfer functions which have been developed by the author and his colleagues over the last five years. With the 3-D transfer functions, resolving power in 3-D confocal imaging can be defined in a unified way, different optical arrangements can be compared with an insight into their inter-relationship, and images of thick objects can be modeled in terms of the Fourier transform which makes the analysis easy. The aim of this book is to provide a systematic introduction to the concept of the 3-D transfer functions in various confocal microscopes, to describe the methods for the derivation of different 3-D transfer functions, and to explain the principles of 3-D confocal imaging in terms of these functions.



The White Confocal


The White Confocal
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Author : Rolf Theodor Borlinghaus
language : en
Publisher: Springer
Release Date : 2017-05-25

The White Confocal written by Rolf Theodor Borlinghaus and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-05-25 with Science categories.


This book offers a comprehensive introduction to confocal microscopy – with a particular focus on spectral confocal microscopy. Beginning with an introduction to optical lenses, it provides a guide to compound microscopes and explains related topics like microscopic resolution. It then presents an outline of fluorescence and its corresponding implications for microscopy. The following excursus on the confocal beam paths includes implementation of acousto-optical devices and modern sensor techniques. Complex relationships are explained in a comprehensible manner, supported by many graphical figures. Discussing the principles of magnifying optics and the technical fundamentals and modes of operation of modern laser scanning microscopes, it is a valuable resource for student and lab technicians as well as faculty members.



Confocal Microscopy


Confocal Microscopy
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Author : Tony Wilson
language : en
Publisher:
Release Date : 1990

Confocal Microscopy written by Tony Wilson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with Science categories.


Numerous applications of confocal microscopes include the life sciences, ophthamology, industrial inspection and semiconductor linewidth metrology. Concentrating on the science and applications of confocal microscopy, this book includes all the latest developments in three-dimensional processing techniques.



Cell Biological Applications Of Confocal Microscopy


Cell Biological Applications Of Confocal Microscopy
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Author :
language : en
Publisher: Elsevier
Release Date : 2003-01-04

Cell Biological Applications Of Confocal Microscopy written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-01-04 with Science categories.


This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Includes detailed protocols accessible to new users Details how to set up and run a "Confocal Microscope Core Facility" Contains over 170 figures



Basic Confocal Microscopy


Basic Confocal Microscopy
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Author : W. Gray (Jay) Jerome
language : en
Publisher: Springer
Release Date : 2018-10-30

Basic Confocal Microscopy written by W. Gray (Jay) Jerome and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-30 with Science categories.


Basic Confocal Microscopy, Second Edition builds on the successful first edition by keeping the same format and reflecting relevant changes and recent developments in this still-burgeoning field. This format is based on the Confocal Microscopy Workshop that has been taught by several of the authors for nearly 20 years and remains a popular workshop for gaining basic skills in confocal microscopy. While much of the information concerning fluorescence and confocal microscopy that made the first edition a success has not changed in the six years since the book was first published, confocal imaging is an evolving field and recent advances in detector technology, operating software, tissue preparation and clearing, image analysis, and more have been updated to reflect this. Several of these advances are now considered routine in many laboratories, and others such as super resolution techniques built on confocal technology are becoming widely available.



Introduction To Optical Microscopy


Introduction To Optical Microscopy
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Author : Jerome Mertz
language : en
Publisher: Cambridge University Press
Release Date : 2019-08

Introduction To Optical Microscopy written by Jerome Mertz and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08 with Medical categories.


Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.



Understanding Light Microscopy


Understanding Light Microscopy
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Author : Jeremy Sanderson
language : en
Publisher: John Wiley & Sons
Release Date : 2019-03-04

Understanding Light Microscopy written by Jeremy Sanderson and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-03-04 with Science categories.


Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.



Basic Confocal Microscopy


Basic Confocal Microscopy
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Author : Robert L. Price
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-04-11

Basic Confocal Microscopy written by Robert L. Price and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-04-11 with Science categories.


Most researchers agree that biological confocal microscopy was jump-started by the confocal design first published by White and Amos in 1985 in the Journal of Cell Biology. As a result, this remains a relatively young field. Yet the use of the technique has grown phenomenally since those early efforts, with new users joining the ranks daily. The publication of Basic Confocal Microscopy reflects the burgeoning need to train new students, technologists, and faculty wishing to use confocal microscopy in their research. A direct outgrowth of the authors’ five-day intensive course in the subject begun in 2005, this book covers the basics and includes all the information required to design, implement, and interpret the results of, biological experiments based on confocal microscopy. Concise yet comprehensive, the volume begins by covering the core issues of fluorescence, specimen preparation and labeling, before moving on to address the analog-to-digital conversion of specimen data gathered using confocal microscopy. Subsequent chapters detail the practicalities of operating confocal microscopes, providing all the information necessary to begin practicing confocal microscopy as well as optimizing the material obtained. The final block of chapters examine 3-dimensional analysis and the reconstruction of data sets, outline some of the ethical considerations in confocal imaging, and then supply a number of resources that the authors have found useful in their own work. Once readers have mastered the information this book presents, the resources found in its pages will be an excellent guide to continued learning about the more advanced forms of confocal microscopy.