Introduction To Fluorescence Correlation Spectroscopy
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An Introduction To Fluorescence Correlation Spectroscopy
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Author : Thorsten Wohland
language : en
Publisher:
Release Date : 2020
An Introduction To Fluorescence Correlation Spectroscopy written by Thorsten Wohland and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020 with Fluorescence spectroscopy categories.
"An Introduction to Fluorescence Correlation Spectroscopy represents a comprehensive introduction to fluorescence correlation spectroscopy (FCS), a biophysical experimental technique increasingly used to study and quantify molecular mobility, concentrations and interactions in vitro, as well as in living cells and multicellular organisms. Students and researchers who are new to FCS can use the book as the first introduction to the technique, while those who are already using FCS regularly in their research may find it useful to deepen their understanding of the technique, its possibilities, limitations, potential pitfalls, and ways to avoid them. This book introduces the reader to all aspects of FCS needed for practical usage of the technique in their research. In the beginning the concept of fluorescence intensity fluctuations and their auto- and cross-correlation functions are explained to give readers an understanding of the underlying principles. This is followed by an overview of instrumental FCS setups and various ways of data collection and processing, the derivations of theoretical models relating the experimentally obtained correlation functions to the underlying molecular processes, and the description of the fitting of experimental data with those models. Mathematically more involved portions are separated from the rest of the text and can be easily skipped by readers more interested in the conceptual and practical aspects of FCS. The book contains interactive graphics and is accompanied by an interactive computable document file allowing the reader to test the dependence of FCS results on a variety of experimental parameters, and to gain practical insights into FCS data fitting. Part of Biophysical Society-IOP series." -- Prové de l'editor.
Fluorescence Correlation Spectroscopy
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Author : R. Rigler
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Fluorescence Correlation Spectroscopy written by R. Rigler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Fluorescence correlation spectroscopy (FCS) was developed in order to char acterize the dynamics of molecular processes in systems in thermodynamic equilibrium. FCS determines transport and chemical reaction rates from mea surements of spontaneous microscopic thermally driven molecular concentra tion fluctuations. Since its inception, and particularly in recent years, techni cal and conceptual advances have extended the range of practical applicability and the information obtainable from FCS measurements. Improvements in microscopy, data acquisition, and data processing have greatly shortened the time required for FCS measurements. FCS can now be routinely applied to labile systems such as cells, and for the acquisition of large volumes of data as required for high-throughput screening. Cross correlation methods pro vide a powerful tool for characterizing interactions among different molecular species. Analysis of the amplitude of concentration fluctuations can provide a wealth of information about aggregation/polymerization process and the compositions of mixtures. Furthermore, FCS provides a bridge between conventional measurements of dynamic processes on a macroscopic concentration scale and the currently developing field of single molecule measurements. Both FCS and single mole cule approaches measure directly stochastic fluctuations in molecular pro perties, and so must be analyzed by statistical methods to yield conventional phenomenological parameters. As commonly practiced, FCS yields these phe nomenological parameters, e. g. , diffusion coefficients and chemical rate con stants, directly in terms of a fluorescence fluctuation autocorrelation func tion.
An Introduction To Fluorescence Correlation Spectroscopy
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Author : Thorsten Wohland
language : en
Publisher: Iop Expanding Physics
Release Date : 2020-11-04
An Introduction To Fluorescence Correlation Spectroscopy written by Thorsten Wohland and has been published by Iop Expanding Physics this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-11-04 with Science categories.
An Introduction to Fluorescence Correlation Spectroscopy represents a comprehensive introduction to fluorescence correlation spectroscopy (FCS), a biophysical experimental technique increasingly used to study and quantify molecular mobility, concentrations and interactions in vitro, as well as in living cells and multicellular organisms. Students and researchers who are new to FCS can use the book as the first introduction to the technique, while those who are already using FCS regularly in their research may find it useful to deepen their understanding of the technique, its possibilities, limitations, and potential pitfalls as well as ways to avoid them. This book introduces the reader to all aspects of FCS needed for practical usage of the technique in their research. In the beginning the concept of fluorescence intensity fluctuations and their auto- and cross-correlation functions are explained to give readers an understanding of the underlying principles. This is followed by an overview of instrumental FCS setups and various ways of data collection and processing, the derivations of theoretical models relating the experimentally obtained correlation functions to the underlying molecular processes, and the description of the fitting of experimental data with those models. Mathematically more involved portions are separated from the rest of the text and can be easily skipped by readers more interested in the conceptual and practical aspects of FCS. The book contains interactive graphics and is accompanied by an interactive computable document file allowing the reader to test the dependence of FCS results on a variety of experimental parameters, and to gain practical insights into FCS data fitting. Key Features Introduces the concepts of FCS in an accessible way, supported by animations and graphics in the ebook. Includes a supplementary interactive computable document file that allows the reader to experiment with various FCS setup and fit parameters, allowing readers to test their understanding and simulate experimental outcomes. Provides rigorous mathematical derivations of fundamental FCS equations and models. Pedagogical features include questions, short reviews and critical discussions of literature relevant to the particular chapter that include applications and fundamental developments in the field of FCS.
An Introduction To Fluorescence Correlation Spectroscopy
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Author : Thorsten Wohland
language : en
Publisher:
Release Date : 2020
An Introduction To Fluorescence Correlation Spectroscopy written by Thorsten Wohland and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020 with SCIENCE categories.
An Introduction to Fluorescence Correlation Spectroscopy represents a comprehensive introduction to fluorescence correlation spectroscopy (FCS), a biophysical experimental technique increasingly used to study and quantify molecular mobility, concentrations and interactions in vitro, as well as in living cells and multicellular organisms. Students and researchers who are new to FCS can use the book as the first introduction to the technique, while those who are already using FCS regularly in their research may find it useful to deepen their understanding of the technique, its possibilities, limitations, potential pitfalls, and ways to avoid them. This book introduces the reader to all aspects of FCS needed for practical usage of the technique in their research. In the beginning the concept of fluorescence intensity fluctuations and their auto- and cross-correlation functions are explained to give readers an understanding of the underlying principles. This is followed by an overview of instrumental FCS setups and various ways of data collection and processing, the derivations of theoretical models relating the experimentally obtained correlation functions to the underlying molecular processes, and the description of the fitting of experimental data with those models. Mathematically more involved portions are separated from the rest of the text and can be easily skipped by readers more interested in the conceptual and practical aspects of FCS. The book contains interactive graphics and is accompanied by an interactive computable document file allowing the reader to test the dependence of FCS results on a variety of experimental parameters, and to gain practical insights into FCS data fitting. Part of Biophysical Society-IOP series.
Introduction To Fluorescence Correlation Spectroscopy
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Author : Elliot L. Elson
language : en
Publisher: CRC PressI Llc
Release Date : 2009-08-15
Introduction To Fluorescence Correlation Spectroscopy written by Elliot L. Elson and has been published by CRC PressI Llc this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-08-15 with Science categories.
Introduction To Fluorescence
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Author : David M. Jameson
language : en
Publisher: CRC Press
Release Date : 2025-03-26
Introduction To Fluorescence written by David M. Jameson and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-03-26 with Science categories.
The phenomenon known as ‘fluorescence’ is now widely used in the chemical, physical and life sciences largely due to the development of highly sophisticated fluorescent probe chemistries and the commercial availability of these probes as well as the development of novel microscopy approaches. This Second Edition of Introduction to Fluorescence helps readers acquire a thorough understanding of basic fluorescence theory and practice. It describes the general principles in a direct way and uses examples from a variety of disciplines to demonstrate them. Since the First Edition, significant advances in the field have appeared. For example, phasors, both lifetime and spectral phasors, have become very popular, and so a new chapter dedicated to this topic has been added in this edition. Furthermore, significant advances have been made in fluorescence microscopy methods, including super-resolution and single-molecule techniques. In color throughout, the book takes readers through the history of important discoveries to the most current advances. It introduces the fundamentals of the fluorescence phenomenon and gives detailed examples of fluorescence applications in the molecular life sciences, including biochemistry, biophysics, clinical chemistry and diagnostics, pharmaceutical science, and cell and molecular biology. The author presents the basic theories underlying the applications and offers in-depth information on practical aspects. Numerous references are given in each chapter, along with a list of particularly important references at the end of each chapter. The text incorporates more than 340 figures that clearly illustrate the concepts and gives the chemical structures of the most widely used fluorescent molecules. In addition, Chapter 13, the Appendix, provides a "Rogue’s Gallery" of the most common errors and pitfalls to avoid.
Principles Of Fluorescence Spectroscopy
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Author : Joseph R. Lakowicz
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-12-05
Principles Of Fluorescence Spectroscopy written by Joseph R. Lakowicz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-05 with Science categories.
The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.
An Introduction To Fluorescence Correlation Spectroscopy
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Author : Thorsten Wohland
language : en
Publisher: Myprint
Release Date : 2020-11-05
An Introduction To Fluorescence Correlation Spectroscopy written by Thorsten Wohland and has been published by Myprint this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-11-05 with categories.
An Introduction to Fluorescence Correlation Spectroscopy represents a comprehensive introduction to fluorescence correlation spectroscopy (FCS), a biophysical experimental technique increasingly used to study and quantify molecular mobility, concentrations and interactions in vitro, as well as in living cells and multicellular organisms. Students and researchers who are new to FCS can use the book as the first introduction to the technique, while those who are already using FCS regularly in their research may find it useful to deepen their understanding of the technique, its possibilities, limitations, and potential pitfalls as well as ways to avoid them. This book introduces the reader to all aspects of FCS needed for practical usage of the technique in their research. In the beginning the concept of fluorescence intensity fluctuations and their auto- and cross-correlation functions are explained to give readers an understanding of the underlying principles. This is followed by an overview of instrumental FCS setups and various ways of data collection and processing, the derivations of theoretical models relating the experimentally obtained correlation functions to the underlying molecular processes, and the description of the fitting of experimental data with those models. Mathematically more involved portions are separated from the rest of the text and can be easily skipped by readers more interested in the conceptual and practical aspects of FCS. The book contains interactive graphics and is accompanied by an interactive computable document file allowing the reader to test the dependence of FCS results on a variety of experimental parameters, and to gain practical insights into FCS data fitting. Key Features Introduces the concepts of FCS in an accessible way, supported by animations and graphics in the ebook. Includes a supplementary interactive computable document file that allows the reader to experiment with various FCS setup and fit parameters, allowing readers to test their understanding and simulate experimental outcomes. Provides rigorous mathematical derivations of fundamental FCS equations and models. Pedagogical features include questions, short reviews and critical discussions of literature relevant to the particular chapter that include applications and fundamental developments in the field of FCS.
New Trends In Fluorescence Spectroscopy
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Author : Bernard Valeur
language : en
Publisher: Springer Science & Business Media
Release Date : 2001
New Trends In Fluorescence Spectroscopy written by Bernard Valeur and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Medical categories.
This first volume in the new Springer Series on Fluorescencebrings together fundamental and applied research from this highly interdisciplinary field ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of state-of-the-art articles will serve as an invaluable tool for practitioners and novices and provide inspiration for new developments and applications.
Handbook Of Fluorescence Spectroscopy And Imaging
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Author : Markus Sauer
language : en
Publisher: John Wiley & Sons
Release Date : 2010-12-23
Handbook Of Fluorescence Spectroscopy And Imaging written by Markus Sauer and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-12-23 with Science categories.
Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.