Introduction To Fluorescence Spectroscopy
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Introduction To Fluorescence Spectroscopy
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Author : Ashutosh Sharma
language : en
Publisher: Wiley-Interscience
Release Date : 1999-05-21
Introduction To Fluorescence Spectroscopy written by Ashutosh Sharma and has been published by Wiley-Interscience this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-05-21 with Science categories.
An accessible guide to all aspects of molecular fluorescence spectroscopy This book introduces the uninitiated reader to the growing body of analytical methods based on molecular fluorescence. Geared to practitioners with no particular training or exposure to the field, it highlights fluorescence spectroscopy's tremendous appeal in present-day pharmaceutical, biomedical, and environmental analysis. Written by two highly respected experts in the field, Introduction to Fluorescence Spectroscopy covers all aspects of the technology-physical fundamentals, instrumentation, methods, and applications. The information is offered at 0a very practical level and addresses a broad range of chemical, physical, biological, and geological problems. The authors incorporate recent advances in commercially available instrumentation as well as fluorescent derivatizing agents, provide many examples of state-of-the-art applications, and discuss future trends. Concise, accessible, up-to-date, Introduction to Fluorescence Spectroscopy is an indispensable reference and an invaluable primer for those involved in the field of analytical science and other professionals interested in this fast-evolving analytical technique.
Principles Of Fluorescence Spectroscopy
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Author : Joseph R. Lakowicz
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Principles Of Fluorescence Spectroscopy written by Joseph R. Lakowicz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.
Fluorescence Spectroscopy
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Author : Carl-Gustaf Rosén
language : en
Publisher: Marcel Dekker
Release Date : 1971
Fluorescence Spectroscopy written by Carl-Gustaf Rosén and has been published by Marcel Dekker this book supported file pdf, txt, epub, kindle and other format this book has been release on 1971 with Science categories.
Introduction To Fluorescence Sensing
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Author : Alexander P. Demchenko
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-12-21
Introduction To Fluorescence Sensing written by Alexander P. Demchenko and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-12-21 with Medical categories.
Fluorescence sensing is a rapidly developing field of research and technology. Its target is nearly the whole world of natural and synthetic compounds being detected in different media including living bodies. The application area range from control of industrial processes to environment monitoring and clinical diagnostics. Among different detection methods fluorescence techniques are distinguished by ultimate sensitivity, high temporal and spatial resolution and versatility that allows not only remote detection of different targets but their imaging within the living cells. The basic mechanism of sensing is the transmission of the signal produced by molecular interaction with the target to fluorescent molecules, nanoparticles and nanocomposites with the detection by devices based on modern electronics and optics. In this interdisciplinary field of research and development the book is primarily intended to be a guide for students and young researchers. It is also addressed to professionals involved in active research and product development serving as a reference for the recent achievements. The users of these products will find description of principles that could allow proper selection of sensors for particular needs. Making a strong link between education, research and product development, this book discusses future directions.
Introduction To Fluorescence
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Author : David M. Jameson
language : en
Publisher: Taylor & Francis
Release Date : 2014-01-22
Introduction To Fluorescence written by David M. Jameson and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-22 with Science categories.
The phenomenon known as fluorescence is now widely used in the chemical and life sciences largely due to the development of highly sophisticated fluorescent probe chemistries and the commercial availability of these probes as well as the development of novel microscopy approaches. Introduction to Fluorescence helps readers acquire a sound understanding of basic fluorescence theory and practice. It describes general principles in a straightforward way and uses examples from a variety of disciplines to demonstrate them. In color throughout, the book takes readers through the history of important discoveries to the most current advances. It introduces the fundamentals of the fluorescence phenomenon and gives detailed examples of fluorescence applications in the molecular life sciences, including biochemistry, biophysics, clinical chemistry and diagnostics, pharmaceutical science, and cell and molecular biology. The author presents the basic theories underlying the applications and offers in-depth information on practical aspects. Along with a list of references in each chapter, the text incorporates more than 250 figures that clearly illustrate the concepts and gives the chemical structures of the most widely used fluorescent molecules. In addition, the appendix provides a "Rogue’s Gallery" of the most common errors and pitfalls to avoid.
Principles And Applications Of Fluorescence Spectroscopy
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Author : Jihad Rene Albani
language : en
Publisher: John Wiley & Sons
Release Date : 2008-04-15
Principles And Applications Of Fluorescence Spectroscopy written by Jihad Rene Albani and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-04-15 with Science categories.
Fluorescence spectroscopy is an important investigational tool in many areas of analytical science, due to its extremely high sensitivity and selectivity. With many uses across a broad range of chemical, biochemical and medical research, it has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems with extremely high resolution. It is particularly heavily used in the pharmaceutical industry where it has almost completely replaced radiochemical labelling. Principles and Applications of Fluorescence Spectroscopy gives the student and new user the essential information to help them to understand and use the technique confidently in their research. By integrating the treatment of absorption and fluorescence, the student is shown how fluorescence phenomena arise and how these can be used to probe a range of analytical problems. A key element of the book is the inclusion of practical laboratory experiments that illustrate the fundamental points and applications of the technique.
An Introduction To Fluorescence Correlation Spectroscopy
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Author : Thorsten Wohland
language : en
Publisher: Myprint
Release Date : 2020-11-05
An Introduction To Fluorescence Correlation Spectroscopy written by Thorsten Wohland and has been published by Myprint this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-11-05 with categories.
An Introduction to Fluorescence Correlation Spectroscopy represents a comprehensive introduction to fluorescence correlation spectroscopy (FCS), a biophysical experimental technique increasingly used to study and quantify molecular mobility, concentrations and interactions in vitro, as well as in living cells and multicellular organisms. Students and researchers who are new to FCS can use the book as the first introduction to the technique, while those who are already using FCS regularly in their research may find it useful to deepen their understanding of the technique, its possibilities, limitations, and potential pitfalls as well as ways to avoid them. This book introduces the reader to all aspects of FCS needed for practical usage of the technique in their research. In the beginning the concept of fluorescence intensity fluctuations and their auto- and cross-correlation functions are explained to give readers an understanding of the underlying principles. This is followed by an overview of instrumental FCS setups and various ways of data collection and processing, the derivations of theoretical models relating the experimentally obtained correlation functions to the underlying molecular processes, and the description of the fitting of experimental data with those models. Mathematically more involved portions are separated from the rest of the text and can be easily skipped by readers more interested in the conceptual and practical aspects of FCS. The book contains interactive graphics and is accompanied by an interactive computable document file allowing the reader to test the dependence of FCS results on a variety of experimental parameters, and to gain practical insights into FCS data fitting. Key Features Introduces the concepts of FCS in an accessible way, supported by animations and graphics in the ebook. Includes a supplementary interactive computable document file that allows the reader to experiment with various FCS setup and fit parameters, allowing readers to test their understanding and simulate experimental outcomes. Provides rigorous mathematical derivations of fundamental FCS equations and models. Pedagogical features include questions, short reviews and critical discussions of literature relevant to the particular chapter that include applications and fundamental developments in the field of FCS.
Fluorescence Spectroscopy
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Author : Amadeo J. Pesce
language : en
Publisher:
Release Date : 1996
Fluorescence Spectroscopy written by Amadeo J. Pesce and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with Fluorescence spectroscopy categories.
New Trends In Fluorescence Spectroscopy
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Author : Bernard Valeur
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
New Trends In Fluorescence Spectroscopy written by Bernard Valeur and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.
Introduction To Fluorescence Sensing
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Author : Alexander P. Demchenko
language : en
Publisher: Springer
Release Date : 2015-10-06
Introduction To Fluorescence Sensing written by Alexander P. Demchenko and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-10-06 with Medical categories.
Fluorescence is the most popular technique in chemical and biological sensing and this book provides systematic knowledge of basic principles in the design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Its ultimate sensitivity, high temporal and spatial resolution and versatility enables high resolution imaging within living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response, up to the detection of single molecules. Its application areas range from the control of industrial processes to environmental monitoring and clinical diagnostics. Being a guide for students and young researchers, it also addresses professionals involved in basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress.