Lens Epithelial Cell Migrational Model

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An Investigation Of Factors Which Influence Lens Epithelial Cell Migration In An In Vitro Model Using Intra Capsular Intra Ocular Lenses

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Author : Ahmed Mohamad Ramadan El-Osta
language : en
Publisher:
Release Date : 2004

An Investigation Of Factors Which Influence Lens Epithelial Cell Migration In An In Vitro Model Using Intra Capsular Intra Ocular Lenses written by Ahmed Mohamad Ramadan El-Osta and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with categories.

The Effects Of Hyaluronic Acid On Lens Epithelial Cell Migration In Vitro

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Author : David John Haeussler
language : en
Publisher:
Release Date : 2011

The Effects Of Hyaluronic Acid On Lens Epithelial Cell Migration In Vitro written by David John Haeussler and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.

Abstract: Purpose. To determine if canine lens epithelial cells (LEC) contain the hyaluronic acid (HA) receptors CD44 and RHAMM, and if HA can impact posterior capsular opacification (PCO) formation in vitro. Methods. Normal (n=10) and cataractous (n=10) canine LEC were evaluated using immunohistochemistry and RT-PCR for expression of CD44 and RHAMM. In vitro models of PCO formation were used to determine if various HA concentrations altered LEC migration. A one-millimeter scratch was created in cultures of confluent canine LEC. Cells were treated with 0, 0.02, 0.012, or 1.0 mg/mL (n=6 per group) of HA dissolved in culture media. Migration of LEC into the scratch was quantified. Mock cataract surgery was performed on canine cadaver eyes. Capsules were re-distended using one of the following (n=6 per group): PBS only, hydroxypropyl methylcellulose viscoelastic, 1.2% HA (12 mg/ml HA) viscoelastic, or 2.0% (20 mg/ml HA) HA viscoelastic. LEC migration within the capsule was evaluated. Results. Normal and cataractous LEC were positive for CD44 and RHAMM protein and mRNA. CD44 showed a statistically significant increase (p

Lens Epithelium And Posterior Capsular Opacification

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Author : Shizuya Saika
language : en
Publisher: Springer
Release Date : 2014-08-12

Lens Epithelium And Posterior Capsular Opacification written by Shizuya Saika and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-08-12 with Medical categories.

This book is the first to summarize the current knowledge of the cell biology of lens epithelial cells in relation to and in the development of posterior capsular opacification (PCO). PCO remains the most common long-term complication of modern cataract surgery, occurring months or years after cataract surgery, unlike most other complications that tend to arise during or soon after the procedure. Opacification of the posterior capsule appears to be linked to lens epithelial cells that are left behind in the eye during cataract removal. These cells proliferate, migrate across the posterior lens capsule, and undergo changes that result in fibrous or pearl-type opacities in the capsule. The first section of the text explains the molecular mechanism and biology of lens epithelial cells that lead to the incidence of PCO. In the second part, in addition to a description of the mechanism and pathological condition of PCO, surgical methods and devices for preventing PCO are discussed in detail. Lens Epithelium and Capsular Opacification will benefit not only young clinical residents and junior researchers, but also established faculty in the clinical or basic academic field.

Directed Migration Re Orientation And Inhibited Proliferation Of Lens Epithelial Cells In Applied Electric Fields

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Author : Entong Wang
language : en
Publisher:
Release Date : 2001

Directed Migration Re Orientation And Inhibited Proliferation Of Lens Epithelial Cells In Applied Electric Fields written by Entong Wang and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with categories.

Capsular Tension Ring Effect On Canine Lens Epithelial Cell Proliferation And Migration Ex Vivo And In Vivo

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Author : Sarah G. Stone
language : en
Publisher:
Release Date : 2008

Capsular Tension Ring Effect On Canine Lens Epithelial Cell Proliferation And Migration Ex Vivo And In Vivo written by Sarah G. Stone and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Cataract categories.

Abstract: Posterior capsule opacification (PCO) is a vision-threatening complication that develops post-cataract surgery in 100% of dogs. The capsular tension ring (CTR) is an intracapsular device implanted during cataract surgery that decreases human PCO clinically. The purposes of the present study were to determine the CTR effect on canine lens epithelial cell (LEC) proliferation and migration in an ex vivo model and on clinical PCO in canine cataract patients and also to determine the safety of canine CTR use. The ex vivo study revealed that CTR and IOL implantation together decreased LEC migration and LEC density on the posterior lens capsule up to day 14 of culture in comparison with single implant or control capsules. In vivo, the CTR does not statistically significantly decrease the extent of PCO up to 4 months post-cataract surgery, and there is no statistically greater risk of intra- or post-operative complications in eyes receiving CTRs.

Lens Epithelial Cell Migrational Model

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Author : Meghan Marshall
language : en
Publisher:
Release Date : 2008

Lens Epithelial Cell Migrational Model written by Meghan Marshall and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with categories.

Metformin Inhibits Epithelial To Mesenchymal Transition In Lens Epithelial Cells

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Author : Jade Marie Edenvirg Lasiste
language : en
Publisher:
Release Date : 2017

Metformin Inhibits Epithelial To Mesenchymal Transition In Lens Epithelial Cells written by Jade Marie Edenvirg Lasiste and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.

"Purpose: Posterior capsule opacification (PCO) is the most common complication after cataract surgery, affecting up to 50% of patients 2-5 years post-operatively. Epithelial-to-mesenchymal transition (EMT) is the main pathophysiology underlying PCO. Metformin, a drug with an excellent safety profile used primarily for diabetes, has been shown to suppress EMT. The objective of this study was to test the effectiveness of metformin in inhibiting EMT in an in vitro model of PCO. Methods: The human lens epithelial cell (LEC) line HLE-B3 was exposed to transforming growth factor-beta (TGF-[beta]) and fibroblast growth factor (FGF) to induce EMT. Subsequently, the effect of metformin on the following cellular parameters were determined: (1) survival, using a viability assay and drug concentrations ranging from 0-100 mM; (2) expression of epithelial (Pax6, E-cadherin) and mesenchymal ([alpha]-smooth muscle actin or [alpha]-SMA, fibronectin) markers via Western blot; (3) morphology, via microscopy and image analysis; (4) and migration, using the wound assay. The presence of the metformin uptake receptor SLC22A1 in the cell line and the ratio of active to inactive protein kinase B (pAkt/Akt) were assessed via Western blot. SLC22A1 expression was confirmed with immunohistochemistry on donor eyes. Statistical analysis of variance (ANOVA) with Tukey post-hoc test was done for analysis of cytotoxicity, morphology and migration data. Results: Metformin is lethal to half (LC50) and all cells at 30 and 80 mM, respectively. A decrease in viability (P

Ocular Pathology

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Author : Myron Yanoff
language : en
Publisher:
Release Date : 2002

Ocular Pathology written by Myron Yanoff and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Medical categories.

The new, 5th Edition of the premier ocular pathology resource offers the latest information in the field. Over 2095 illustrations *1800 in full color* from the authors' collections represent one of the finest compilations of rare and unusual ocular conditions available. Comprehensive, yet concise, it explores new topics such as complications of corneal surgery, vascular disorders, secondary cataracts, bone-marrow transplant retinopathy, neural retinal detachment, ocular melanotic tumors, and more. Plus, a companion CD-ROM and text/CD-ROM package are available! Explores new topics such as complications of corneal surgery, vascular disorders, secondary cataracts, bone-marrow transplant retinopathy, neural retinal detachment, and ocular melanotic tumors (including diabetes). Offers expanded coverage of entities such as chromosome 17 deletion syndrome, necrobiotic xanthogranuloma, climatic protoglycan stromal keratopathy, unilateral acute idiopathic maculopathy, acute mutifocal retinitis, idiopathic polypoidal choroidal vasculopathy, North Carolina macular dystrophy, familial internal limiting membrane dystrophy, clumped pigmentary retinal dystrophy (clumped pigmentary retinal degeneration), vasoproliferative retinal tumors, Schwartz's syndrome, familial amyloidotic polyneuropathy, familial atypical mole and melanoma (FAM-M) syndrome, and more! Features specific genetic profiles for many existing entities.

Webvision

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Author : Helga Kolb
language : en
Publisher:
Release Date : 2007

Webvision written by Helga Kolb and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with categories.

Animal Models In Eye Research

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Author :
language : en
Publisher: Academic Press
Release Date : 2011-04-28

Animal Models In Eye Research written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-04-28 with Science categories.

The eye is a complex sensory organ, which enables visual perception of the world. Thus the eye has several tissues that do different tasks. One of the most basic aspects of eye function is the sensitivity of cells to light and its transduction though the optic nerve to the brain. Different organisms use different ways to achieve these tasks. In this sense, eye function becomes a very important evolutionary aspect as well. This book presents the different animal models that are commonly used for eye research and their uniqueness in evaluating different aspects of eye development, evolution, physiology and disease. Presents information on the major animal models used in eye research including invertebrates and vertebrates Provides researchers with information needed to choose between model organisms Includes an introductory chapter on the different types of eyes, stressing possible common molecular machinery