Lipid Nanoparticle Messenger Rna Formulations Against Infections
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Lipid Nanoparticle Messenger Rna Formulations Against Infections
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Author : Xucheng Hou
language : en
Publisher:
Release Date : 2022
Lipid Nanoparticle Messenger Rna Formulations Against Infections written by Xucheng Hou and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022 with Messenger RNA categories.
The rapid development and successful application of messenger RNA (mRNA) vaccines to ameliorate the coronavirus disease 2019 (COVID-19) pandemic highlight the potential of mRNA therapeutics to prevent and treat infectious diseases. To further facilitate the clinical translation of mRNA therapeutics, additional efforts are required to optimize targeted delivery systems, mRNA stability, manufacturing costs, and applications beyond severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines. The objective of this dissertation is to develop novel lipid nanoparticle (LNP)-mRNA formulations against bacterial and viral infections via synthesis of effective delivery materials to engineer macrophages, de novo design of mRNA structures to improve the stability and translational efficiency, and optimization of long-term storage conditions of LNP-mRNA formulations. Chapter 1 briefly reviews the strategies for optimizing mRNA structures, the representative LNPs for mRNA delivery, and the applications of LNP-mRNA formulations in infectious diseases. Chapter 2 demonstrates that the adoptive transfer of macrophages pre-storing antimicrobial peptides in their lysosomes (MACs) can be used to treat sepsis induced by multidrug-resistant bacteria. The MACs were constructed by ex vivo engineering with vitamin C LNPs encapsulating antimicrobial peptide linked cathepsin B (AMP-CatB) mRNA. The AMP-CatB mRNA enabled the accumulation of AMPs in macrophage lysosomes, a crucial organelle for bactericidal activities. In vivo data revealed that the adoptive MAC transfer reduced bacterial burden, resulting in the complete recovery of septic mice with immunosuppression. Chapter 3 describes the development of a COVID-19 vaccine candidate with optimized untranslated regions (UTRs) in mRNA. After bioinformatics analysis and de novo design of the UTRs, the optimal combination of 5’ and 3’ UTRs was identified and named as NASAR, which increased five to ten-fold mRNA translation compared to wild type UTRs. Next, a COVID-19 vaccine candidate was developed by formulating NASAR mRNA encoding SARS-CoV-2 antigens with N1, N3, N5-tris(3-(didodecylamino)propyl)benzene-1,3,5-tricarboxamide (TT3) LNPs. After a prime-boost vaccination, the vaccine induced strong spike-specific antibody responses in animal models. Chapter 4 investigates the conditions for long-term storage of LNP-mRNA formulations. The stability of TT3 LNPs was evaluated under freezing or lyophilization conditions with a series of concentrations of cryoprotectants (sucrose, trehalose, or mannitol). Through evaluating the in vitro and in vivo mRNA delivery efficacy, we identified that the supplement with 5% (w/v) sucrose or trehalose to TT3 LNPs maintained the mRNA delivery efficacy for three months under the liquid nitrogen storage condition. Collectively, these results demonstrate that LNP-mRNA formulations are promising tools for preventing and treating infections with clinical transferability.
Intracellular Delivery
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Author : Aleš Prokop
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-05-26
Intracellular Delivery written by Aleš Prokop and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-05-26 with Science categories.
This book features a special subsection of Nanomedicine, an application of nanotechnology to achieve breakthroughs in healthcare. It exploits the improved and often novel physical, chemical and biological properties of materials only existent at the nanometer scale. As a consequence of small scale, nanosystems in most cases are efficiently uptaken by cells and appear to act at the intracellular level. Nanotechnology has the potential to improve diagnosis, treatment and follow-up of diseases, and includes targeted drug delivery and regenerative medicine; it creates new tools and methods that impact significantly upon existing conservative practices. This volume is a collection of authoritative reviews. In the introductory section we define the field (intracellular delivery). Then, the fundamental routes of nanodelivery devices, cellular uptake, types of delivery devices, particularly in terms of localized cellular delivery, both for small drug molecules, macromolecular drugs and genes; at the academic and applied levels, are covered. The following section is dedicated to enhancing delivery via special targeting motifs followed by the introduction of different types of intracellular nanodelivery devices (e.g. a brief description of their chemistry) and ways of producing these different devices. Finally, we put special emphasis on particular disease states and on other biomedical applications, whilst diagnostic and sensing issues are also included. Intracellular delivery / therapy is a highly topical which will stir great interest. Intracellular delivery enables much more efficient drug delivery since the impact (on different organelles and sites) is intracellular as the drug is not supplied externally within the blood stream. There is great potential for targeted delivery with improved localized delivery and efficacy.
Rna Nanotechnology
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Author : Bin Wang
language : en
Publisher: CRC Press
Release Date : 2014-04-02
Rna Nanotechnology written by Bin Wang and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-04-02 with Medical categories.
In the past few decades there has been incredible growth in "bionano"-related research, which has been accompanied by numerous publications in this field. Although various compilations address topics related to deoxyribonucleic acid (DNA) and protein, there are few books that focus on determining the structure of ribonucleic acid (RNA) and using RNA as building blocks to construct nanoarchitectures for biomedical and healthcare applications. RNA Nanotechnology is a comprehensive volume that details both the traditional approaches and the latest developments in the field of RNA-related technology. This book targets a wide audience: a broad introduction provides a solid academic background for students, researchers, and scientists who are unfamiliar with the subject, while the in-depth descriptions and discussions are useful for advanced professionals. The book opens with reviews on the basic aspects of RNA biology, computational approaches for predicting RNA structures, and traditional and emerging experimental approaches for probing RNA structures. This section is followed by explorations of the latest research and discoveries in RNA nanotechnology, including the design and construction of RNA-based nanostructures. The final segment of the book includes descriptions and discussions of the potential biological and therapeutic applications of small RNA molecules, such as small/short interfering RNAs (siRNAs), microRNAs (miRNAs), RNA aptamers, and ribozymes.
Toll Like Receptors Tlrs And Innate Immunity
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Author : Stefan Bauer
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-12-11
Toll Like Receptors Tlrs And Innate Immunity written by Stefan Bauer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-11 with Medical categories.
Overall recent research on TLRs has led to tremendous increase in our understanding of early steps in pathogen recognition and will presumably lead to potent TLR targeting therapeutics in the future. This book reviews and highlights our recent understanding on the function and ligands of TLRs as well as their role in autoimmunity, dendritic cell activation and target structures for therapeutic intervention.
Rna Vaccines
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Author : Thomas Kramps
language : en
Publisher: Humana
Release Date : 2024-06-21
Rna Vaccines written by Thomas Kramps and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-06-21 with Science categories.
This second edition volume expands on the previous edition with discussions of the latest technology and advancements in the development of RNA vaccines and proposed solutions on how to tackle currently unmet medical needs. The chapters in this book are organized into four parts and cover topics such as replication and non-replicating RNA vectors, formulation and delivery of RNA vaccines, and clinical and nonclinical development of RNA vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensive, RNA Vaccines: Methods and Protocols, Second Edition is a valuable resource for any researcher interested in learning more about this important and developing field.
Silk Based Drug Delivery Systems
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Author : Elia Bari
language : en
Publisher: Royal Society of Chemistry
Release Date : 2020-10-07
Silk Based Drug Delivery Systems written by Elia Bari and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-07 with Science categories.
Covering spider silk and silk worm cocoons, the editors elucidate the extraction, structure and properties of silk sericin and silk fibroin.
Stealth Liposomes
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Author : Danilo D. Lasic
language : en
Publisher: CRC Press
Release Date : 2018-05-04
Stealth Liposomes written by Danilo D. Lasic and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-04 with Medical categories.
This book examines stealth liposomes from a multidisciplinary approach, which includes theoretical polymer physics, organic synthesis, colloid science, and biology. Discussions include theory, chemistry, biochemistry, pharmacology, preclinical studies in model systems, and medical applications in humans.
Lipids And Essential Oils As Antimicrobial Agents
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Author : Halldor Thormar
language : en
Publisher: John Wiley & Sons
Release Date : 2010-12-28
Lipids And Essential Oils As Antimicrobial Agents written by Halldor Thormar and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-12-28 with Science categories.
Lipids and essential oils have strong antimicrobial properties — they kill or inhibit the growth of microbes such as bacteria, fungi, or viruses. They are being studied for use in the prevention and treatment of infections, as potential disinfectants, and for their preservative and antimicrobial properties when formulated as pharmaceuticals, in food products, and in cosmetics. Lipids and Essential Oils as Antimicrobial Agents is a comprehensive review of the scientific knowledge in this field. International experts provide summaries on: the chemical and biological properties of lipids and essential oils use of lipids and essential oils in pharmaceuticals, cosmetics and health foods antimicrobial effects of lipids in vivo and in vitro antimicrobial lipids in milk antimicrobial lipids of the skin antibacterial lipids as sanitizers and disinfectants antibacterial, antifungal, and antiviral activities of essential oils antimicrobial lipids in milk antimicrobial lipids of the skin antibacterial lipids as sanitizers and disinfectants antibacterial, antifungal, and antiviral activities of essential oils Lipids and Essential Oils as Antimicrobial Agents is an essential guide to this important topic for researchers and advanced students in academia and research working in pharmaceutical, cosmetic and food sciences, biochemistry and natural products chemistry, microbiology; and for health care scientists and professionals working in the fields of public health and infectious diseases. It will also be of interest to anyone concerned about health issues and particularly to those who are conscious of the benefits of health food and natural products.
Nano Oncologicals
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Author : Maria José Alonso
language : en
Publisher: Springer
Release Date : 2014-10-15
Nano Oncologicals written by Maria José Alonso and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-10-15 with Medical categories.
This authoritative volume focuses on emerging technologies in cancer nano medicine, characterized by their multi-functionality and potential to address simultaneously diverse issues of clinical relevance in the treatment of cancer. The book consists of sixteen chapters divided into six sections: 1) Biological Barriers in Cancer; 2) Tumor Targeting; 3) Targeting the Immune System; 4) Gene Therapy; 5) Nano theranostics and 6) Translational Aspects of Nano-Oncologicals. The volume starts with an introduction describing the biological barriers associated with cancer therapy and highlighting ways to overcome such barriers through the use of nanotechnology. This is followed by an analysis of the two major targeting strategies currently under investigation in cancer therapy: namely, the targeting of cancer cells and the targeting of the immune system. In the first case, the book presents liposomal and polymer-based therapies, including photodynamic approaches. In the second case, it analyzes in detail the possibility of either improving the efficiency of the immune system toward preventing cancer progression (cancer immunomodulation) or generating responses against specific cancer antigens (cancer vaccines). Beyond these targeting options, Nano-Oncologicals: New Targeting and Delivery Approaches presents the most recent technological advances in the area of nucleic acid-based therapies, along with those in the area of theranostics, where the design of multifunctional nano carriers becomes vital. Following the study of the most promising nanotechnologies around the development of nano-oncologicals, the book ends with an overview of regulatory and toxicological issues, which are critical in their translational pathway, and the presentation of a nucleic acid-based therapy case-study. This book is an important resource for scientists interested in the design and development of anticancer nanotechnologies and also to those aiming to push their technology through clinical development.
Advanced Healthcare Materials
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Author : Ashutosh Tiwari
language : en
Publisher: John Wiley & Sons
Release Date : 2014-05-09
Advanced Healthcare Materials written by Ashutosh Tiwari and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-09 with Technology & Engineering categories.
Offers a comprehensive and interdisciplinary view of cutting-edge research on advanced materials for healthcare technology and applications Advanced healthcare materials are attracting strong interest in fundamental as well as applied medical science and technology. This book summarizes the current state of knowledge in the field of advanced materials for functional therapeutics, point-of-care diagnostics, translational materials, and up-and-coming bioengineering devices. Advanced Healthcare Materials highlights the key features that enable the design of stimuli-responsive smart nanoparticles, novel biomaterials, and nano/micro devices for either diagnosis or therapy, or both, called theranostics. It also presents the latest advancements in healthcare materials and medical technology. The senior researchers from global knowledge centers have written topics including: State-of-the-art of biomaterials for human health Micro- and nanoparticles and their application in biosensors The role of immunoassays Stimuli-responsive smart nanoparticles Diagnosis and treatment of cancer Advanced materials for biomedical application and drug delivery Nanoparticles for diagnosis and/or treatment of Alzheimers disease Hierarchical modelling of elastic behavior of human dental tissue Biodegradable porous hydrogels Hydrogels in tissue engineering, drug delivery, and wound care Modified natural zeolites Supramolecular hydrogels based on cyclodextrin poly(pseudo)rotaxane Polyhydroxyalkanoate-based biomaterials Biomimetic molecularly imprinted polymers