Lipid Nanoparticles For Mrna Mediated Cancer Therapy
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Lipid Nanoparticles For Mrna Mediated Cancer Therapy
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Author : Dania Grant-Serroukh
language : en
Publisher:
Release Date : 2019
Lipid Nanoparticles For Mrna Mediated Cancer Therapy written by Dania Grant-Serroukh and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with categories.
The use of messenger ribonucleic acid (mRNA) to introduce protein sequences into cells, expressing cytokines or antigens, is a promising therapeutic strategy in the oncology field. However, advancement of this technology is stalled by the lack of an effective delivery system. Developing mRNA-specific vectors, along with improving understanding of vector structure and fundamental cellular uptake mechanisms, should allow further progress in moving mRNA therapy to the clinic. This work investigates the use of lipid-peptide based vectors for the delivery of mRNA both in vitro and in vivo. The components of the vector were optimised to produce high levels of transfection in B16 melanoma cells. The resultant formulation comprised of the cationic lipid C14, the phospholipid DOPE, cholesterol and peptide 35. This formulation transfected a number of cancer cells types, producing high levels of expression of luciferase and green fluorescent protein. Formulations were characterised using dynamic light scattering, small-angle X-ray scattering, electron and atomic force microscopy to elucidate the structure of vectors. The nanocomplex had a spherical morphology and appeared to comprise an external lipid membrane with mRNA and/or peptides present on the surface. Optimal particles showed resistance to degradation and aggregation for up to 28 days at 4 oC and showed no loss of activity in the presence of RNase. Finally, mRNA uptake kinetics and trafficking were explored using fluorescent microscopy where no differences between optimal and non-optimal formulations were found; suggesting the key factor affecting performance was endosomal escape. Overall, the peptide-lipid-mRNA system was shown to be a promising platform for further investigation of mRNA delivery in vivo.
Intracellular Delivery
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Author : Aleš Prokop
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-05-26
Intracellular Delivery written by Aleš Prokop and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-05-26 with Science categories.
This book features a special subsection of Nanomedicine, an application of nanotechnology to achieve breakthroughs in healthcare. It exploits the improved and often novel physical, chemical and biological properties of materials only existent at the nanometer scale. As a consequence of small scale, nanosystems in most cases are efficiently uptaken by cells and appear to act at the intracellular level. Nanotechnology has the potential to improve diagnosis, treatment and follow-up of diseases, and includes targeted drug delivery and regenerative medicine; it creates new tools and methods that impact significantly upon existing conservative practices. This volume is a collection of authoritative reviews. In the introductory section we define the field (intracellular delivery). Then, the fundamental routes of nanodelivery devices, cellular uptake, types of delivery devices, particularly in terms of localized cellular delivery, both for small drug molecules, macromolecular drugs and genes; at the academic and applied levels, are covered. The following section is dedicated to enhancing delivery via special targeting motifs followed by the introduction of different types of intracellular nanodelivery devices (e.g. a brief description of their chemistry) and ways of producing these different devices. Finally, we put special emphasis on particular disease states and on other biomedical applications, whilst diagnostic and sensing issues are also included. Intracellular delivery / therapy is a highly topical which will stir great interest. Intracellular delivery enables much more efficient drug delivery since the impact (on different organelles and sites) is intracellular as the drug is not supplied externally within the blood stream. There is great potential for targeted delivery with improved localized delivery and efficacy.
Nano Oncologicals
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Author : Maria José Alonso
language : en
Publisher: Springer
Release Date : 2014-10-15
Nano Oncologicals written by Maria José Alonso and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-10-15 with Medical categories.
This authoritative volume focuses on emerging technologies in cancer nano medicine, characterized by their multi-functionality and potential to address simultaneously diverse issues of clinical relevance in the treatment of cancer. The book consists of sixteen chapters divided into six sections: 1) Biological Barriers in Cancer; 2) Tumor Targeting; 3) Targeting the Immune System; 4) Gene Therapy; 5) Nano theranostics and 6) Translational Aspects of Nano-Oncologicals. The volume starts with an introduction describing the biological barriers associated with cancer therapy and highlighting ways to overcome such barriers through the use of nanotechnology. This is followed by an analysis of the two major targeting strategies currently under investigation in cancer therapy: namely, the targeting of cancer cells and the targeting of the immune system. In the first case, the book presents liposomal and polymer-based therapies, including photodynamic approaches. In the second case, it analyzes in detail the possibility of either improving the efficiency of the immune system toward preventing cancer progression (cancer immunomodulation) or generating responses against specific cancer antigens (cancer vaccines). Beyond these targeting options, Nano-Oncologicals: New Targeting and Delivery Approaches presents the most recent technological advances in the area of nucleic acid-based therapies, along with those in the area of theranostics, where the design of multifunctional nano carriers becomes vital. Following the study of the most promising nanotechnologies around the development of nano-oncologicals, the book ends with an overview of regulatory and toxicological issues, which are critical in their translational pathway, and the presentation of a nucleic acid-based therapy case-study. This book is an important resource for scientists interested in the design and development of anticancer nanotechnologies and also to those aiming to push their technology through clinical development.
Animal Cell Electroporation And Electrofusion Protocols
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Author : Jac A. Nickoloff
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Animal Cell Electroporation And Electrofusion Protocols written by Jac A. Nickoloff and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
The ability to introduce macromolecules into animal cells, includ ing DNA, RNA, proteins, and other bioactive compounds has facili tated a broad range of biological studies, from biochemistry and biophysics to molecular biology, cell biology, and whole animal stud ies. Gene transfer technology in particular will continue to play an essential role in studies aimed at improving our understanding of the relationships between the gene structure and function, and it has impor tant practical applications in both biotechnology and biomedicine, as evidenced by the current intense interest in gene therapy. Although DNA and other macromolecules may be introduced into cells by a variety of methods, including chemical treatments and microinjection, el- troporation has proven to be simpler to perform, more efficient, and effective with a wider variety of cell types than other techniques. The early and broad success of electric field-mediated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, including RNA, enzymes, antibodies, and analytic dyes. Animal Cell Electroporation and Electrofusion Protocols begins with three chapters that describe the theoretical and practical aspects of electroporation, including a review of the commercially available instrumentation. These introductory chapters will be of particular inter est to those new to electric field technologies and to those developing protocols for as yet untested species or cell types. Nineteen chapters follow that present well-tested protocols for electroporation of proteins and DNA into insect, fish, and mammalian cells.
Rna Nanotechnology
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Author : Bin Wang
language : en
Publisher: CRC Press
Release Date : 2014-04-02
Rna Nanotechnology written by Bin Wang and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-04-02 with Medical categories.
In the past few decades there has been incredible growth in "bionano"-related research, which has been accompanied by numerous publications in this field. Although various compilations address topics related to deoxyribonucleic acid (DNA) and protein, there are few books that focus on determining the structure of ribonucleic acid (RNA) and using RNA as building blocks to construct nanoarchitectures for biomedical and healthcare applications. RNA Nanotechnology is a comprehensive volume that details both the traditional approaches and the latest developments in the field of RNA-related technology. This book targets a wide audience: a broad introduction provides a solid academic background for students, researchers, and scientists who are unfamiliar with the subject, while the in-depth descriptions and discussions are useful for advanced professionals. The book opens with reviews on the basic aspects of RNA biology, computational approaches for predicting RNA structures, and traditional and emerging experimental approaches for probing RNA structures. This section is followed by explorations of the latest research and discoveries in RNA nanotechnology, including the design and construction of RNA-based nanostructures. The final segment of the book includes descriptions and discussions of the potential biological and therapeutic applications of small RNA molecules, such as small/short interfering RNAs (siRNAs), microRNAs (miRNAs), RNA aptamers, and ribozymes.
Inhibitors Of Protein Protein Interactions
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Author : Ali Tavassoli
language : en
Publisher: Royal Society of Chemistry
Release Date : 2020-12-07
Inhibitors Of Protein Protein Interactions written by Ali Tavassoli and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-12-07 with Science categories.
Protein-protein interactions (PPI) are at the heart of the majority of cellular processes, and are frequently dysregulated or usurped in disease. Given this central role, the inhibition of PPIs has been of significant interest as a means of treating a wide variety of diseases. However, there are inherent challenges in developing molecules capable of disrupting the relatively featureless and large interfacial areas involved. Despite this, there have been a number of successes in this field in recent years using both traditional drug discovery approaches and innovative, interdisciplinary strategies using novel chemical scaffolds. This book comprehensively covers the various aspects of PPI inhibition, encompassing small molecules, peptidomimetics, cyclic peptides, stapled peptides and macrocycles. Illustrated throughout with successful case studies, this book provides a holistic, cutting-edge view of the subject area and is ideal for chemical biologists and medicinal chemists interested in developing PPI inhibitors.
Drug Delivery Systems In Cancer Therapy
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Author : Dennis M. Brown
language : en
Publisher: Springer Science & Business Media
Release Date : 2003-09-08
Drug Delivery Systems In Cancer Therapy written by Dennis M. Brown and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-09-08 with Medical categories.
Leading experts survey the currently available technologies designed to improve the delivery of today's cancer chemotherapeutic agents. The authors review both the theoretical and practical considerations governing conventional and nonconventional methods of drug administration, and identify promising opportunities for product development. In their outline and discussion of the use of novel formulation technologies-including synthetic polymers and biomaterials for prolonged or sustained drug release to achieve potentially greater therapeutic effect-they profile those technologies that have resulted in a number of approved and late-stage clinical products.
Nanopharmaceuticals Principles And Applications Vol 3
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Author : Vinod Kumar Yata
language : en
Publisher: Springer Nature
Release Date : 2020-08-19
Nanopharmaceuticals Principles And Applications Vol 3 written by Vinod Kumar Yata and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-08-19 with Technology & Engineering categories.
This book is the third volume on this subject and focuses on the recent advances of nanopharmaceuticals in cancer, dental, dermal and drug delivery applications and presents their safety, toxicity and therapeutic efficacy. The book also includes the transport phenomenon of nanomaterials and important pathways for drug delivery applications. It goes on to explain the toxicity of nanoparticles to different physiological systems and methods used to assess this for different organ systems using examples of in vivo systems.
Toll Like Receptors Tlrs And Innate Immunity
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Author : Stefan Bauer
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-12-11
Toll Like Receptors Tlrs And Innate Immunity written by Stefan Bauer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-11 with Medical categories.
Overall recent research on TLRs has led to tremendous increase in our understanding of early steps in pathogen recognition and will presumably lead to potent TLR targeting therapeutics in the future. This book reviews and highlights our recent understanding on the function and ligands of TLRs as well as their role in autoimmunity, dendritic cell activation and target structures for therapeutic intervention.
Therapeutic Oligonucleotides
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Author : Jens Kurreck
language : en
Publisher: Royal Society of Chemistry
Release Date : 2008
Therapeutic Oligonucleotides written by Jens Kurreck and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Medical categories.
This book provides a compelling overall update on current status of RNA interference