Mapping Hybrid Lethal Genes On The X Chromosome Of C Briggsae
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Mapping Hybrid Lethal Genes On The X Chromosome Of C Briggsae
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Author : Blaine E. Bittorf
language : en
Publisher:
Release Date : 2018
Mapping Hybrid Lethal Genes On The X Chromosome Of C Briggsae written by Blaine E. Bittorf and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with Bacterial artificial chromosomes categories.
In the cross of C. nigoni males to C. briggsae hermaphrodites, all F1 males arrest during embryogenesis. However in the reciprocal cross there are some viable F1 male progeny. This unidirectional male-specific lethality in the F1 hybrids has been attributed to a hybrid lethal gene in a 500 Kb region of the X chromosome of C. briggsae. Cbr-him-8 is a recessive maternal suppressor of the male-specific lethal phenotype, due to the requirement of the him-8 protein for proper X chromosome pairing. Without proper pairing of any one of the chromosomes in the Caenorhabditis genome, genes present on the unpaired chromosome will be silenced due to a process known as meiotic silencing of unpaired chromosomes (MSUC). It has been proposed that MSUC-based silencing of the X-linked hybrid lethal gene is the mechanism by which the male-specific lethality is suppressed. Based on this model, a co-suppression assay was used to identify the hybrid lethal gene. Transgenic strains of C. briggsae were constructed via microinjection of bacterial artificial chromosomes (BACs) of small portions of the X chromosome in which the hybrid lethal gene resides. The BACs were mixed with pCFJ909, a plasmid containing a functional cbr-unc-119 gene, this mixture was then microinjected directly into the gonad of cbr-unc-119 mutant hermaphrodites. A proportion of the resulting progeny incorporated the injected DNA into their nucleus and formed heritable extra-chromosomal arrays. These offspring were then selected based on the rescue of the unc-119 phenotype. Transgenic hermaphrodites were then mated to C. nigoni males and scored for viable F1 male progeny. Two BAC rescued the male specific hybrid lethal phenotype. Multiple other BACs failed to rescue the lethality phenotype. Focusing on a single BAC clone, using gene groupings and pCFJ909 the number of possible genes have been narrowed to two candidate hybrid lethal genes within the BAC 08G05. As well as 5 candidate hybrid lethal genes in the non-adjacent BAC 17D03.
Identification Of A Hybrid Lethal Gene On The X Chromosome Of Caenorhabditis Briggsae
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Author : John Kelly Dougherty
language : en
Publisher:
Release Date : 2019
Identification Of A Hybrid Lethal Gene On The X Chromosome Of Caenorhabditis Briggsae written by John Kelly Dougherty and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with Bacterial artificial chromosomes categories.
Two closely related Caenorhabditis species, C. briggsae and C. nigoni are cross fertile and produce viable adult progeny. From C. nigoni mothers, F1 adult females are viable and fertile, F1 males are viable but sterile. In crosses that utilize C. nigoni males and C. briggsae hermaphrodites produce viable adult F1 females but F1 males arrest during embryogenesis. A mutation in the Cbr-him-8 gene is a recessive maternal-effect suppressor of male-specific lethality. Hybrid crosses with cbr-him-8 mutant mothers produce viable adult male progeny. The HIM-8 protein in C. elegans is required for the pairing of X-chromosomes during meiosis. This function is likely conserved in C. briggsae. Unpaired chromosomes are transcriptionally silenced in a wide variety of taxa. Based on this information it's been proposed that the meiotic silencing of unpaired chromosomes (MSUC) is suppressing an X-linked hybrid lethal gene responsible for male specific lethality. Multiple co-suppression assays identified two genes as candidate hybrid lethal genes, CBG30927 and CBG00239. These genes were knocked out with RNAi and CRISPR to evaluate if either of these genes were a hybrid lethal gene. sgRNA/Cas9 complexes and dsRNA of the candidate hybrid lethal genes was injected into C. briggsae hermaphrodites. Injections using CRISPR were able to disrupt expression of control targets but not the candidate hybrid lethal genes. Both RNAi and CRISPR injected nematodes were mated with C. nigoni males and the resulting progeny were scored for viable F1 males. From injections of dsRNA containing copies of CBG30927 or Cbr-hig-1, male progeny were derived. Cbr-hig-1 has a syntenic ortholog in C. nigoni that is not present in any other Caenorhabditis species. Several regions were identified in the C. briggsae and C. nigoni transcripts including an exon 5 extension that is responsible for a change in the predicted structure of the proteins that could be responsible for the dysgenic interactions.
Genetics Of Speciation
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Author : David L. Jameson
language : en
Publisher:
Release Date : 1977
Genetics Of Speciation written by David L. Jameson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1977 with Evolution categories.
The nature of populations, races, subspecies, and species. Genetic basis of isolation. Origin of isolation - theoretical. Origin of isolation - experimental. The nature of the speciation process.
C Elegans Ii
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Author : Donald L. Riddle
language : en
Publisher: Firefly Books
Release Date : 1997
C Elegans Ii written by Donald L. Riddle and has been published by Firefly Books this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Medical categories.
Defines the current status of research in the genetics, anatomy, and development of the nematode C. elegans, providing a detailed molecular explanation of how development is regulated and how the nervous system specifies varied aspects of behavior. Contains sections on the genome, development, neural networks and behavior, and life history and evolution. Appendices offer genetic nomenclature, a list of laboratory strain and allele designations, skeleton genetic maps, a list of characterized genes, a table of neurotransmitter assignments for specific neurons, and information on codon usage. Includes bandw photos. For researchers in worm studies, as well as the wider community of researchers in cell and molecular biology. Annotation copyrighted by Book News, Inc., Portland, OR
Nematodes As Model Organisms
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Author : Itamar Glazer
language : en
Publisher: Cab International
Release Date : 2022
Nematodes As Model Organisms written by Itamar Glazer and has been published by Cab International this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022 with Biological models categories.
"Nematodes, especially Caenorhabditis elegans have been used as a model for research in molecular biology since the 1960's. This is a much-needed update on research on fundamental processes in areas such as genetics, developmental biology, nutrition, toxicology, ecology, pharmacology and medicine"--
Rapidly Evolving Genes And Genetic Systems
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Author : Rama S. Singh
language : en
Publisher: Oxford University Press
Release Date : 2012-06-28
Rapidly Evolving Genes And Genetic Systems written by Rama S. Singh and has been published by Oxford University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-06-28 with Science categories.
A range of theories on the rates of evolution-from static to gradual to punctuated to quantum-have been developed, mostly by comparing morphological changes over geological timescales as described in the fossil record.
Cellular And Molecular Aspects Of Plant Nematode Interactions
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Author : Carmen Fenoll
language : en
Publisher: Springer Science & Business Media
Release Date : 1997
Cellular And Molecular Aspects Of Plant Nematode Interactions written by Carmen Fenoll and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Science categories.
In 1992 a Concerted Action Programme (CAP) was initiated by Peter Sijmons with the purpose of intensifying collaborations between 16 European laboratories working on plant-parasitic nematodes. The four-year programme entitled Resistance Mechanisms Against Plant-Parasitic Nematodes focused on molecular aspects of the interaction between sedentary nematodes and plants on the model system Arabidopsis and on novel resistance strategies. Funding was provided mainly for exchange visits between collaborating laboratories and for the organization of annual meetings. During the last annual meeting which was held in May 1996 in Toledo, Spain, Carmen Fenoll initiated the production of this volume.
Genes In Conflict
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Author : Austin BURT
language : en
Publisher: Harvard University Press
Release Date : 2009-06-30
Genes In Conflict written by Austin BURT and has been published by Harvard University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-06-30 with Science categories.
Covering all species from yeast to humans, this is the first book to tell the story of selfish genetic elements that act narrowly to advance their own replication at the expense of the larger organism.
Insect Molecular Genetics
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Author : Marjorie A. Hoy
language : en
Publisher: Elsevier
Release Date : 2013-10-22
Insect Molecular Genetics written by Marjorie A. Hoy and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-10-22 with Science categories.
Developed as an introduction to new molecular genetic techniques, Insect Molecular Genetics also provides literature, terminology, and additional sources of information to students, researchers, and professional entomologists. Although most molecular genetics studies have employed Drosophila, this book applies the same techniques to other insects, including pest insects of economic importance. As a text, as a reference, as a primer, and as a review of a vast and growing literature, Insect Molecular Genetics is a valuable addition to the libraries of entomologists, geneticists, and molecular biologists. Features offered by this unique reference source: Detailed illustrations Suggested readings at the end of each chapter Glossary of molecular genetic terms
Green Fluorescent Protein
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Author : Martin Chalfie
language : en
Publisher: John Wiley & Sons
Release Date : 2005-11-18
Green Fluorescent Protein written by Martin Chalfie and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-11-18 with Science categories.
Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.