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Maximizing Gene Expression


Maximizing Gene Expression
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Maximizing Gene Expression


Maximizing Gene Expression
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Author : William Reznikoff
language : en
Publisher: Elsevier
Release Date : 2014-05-20

Maximizing Gene Expression written by William Reznikoff and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-20 with Science categories.


Maximizing Gene Expression focuses on prokaryotic and eukaryotic gene expression. The book first discusses E. coli promoters. Topics include structure analysis, steps in transcription initiation, structure-function correlation, and regulation of transcription initiation. The text also highlights yeast promoters, including elements that select initiation sites, transcription regulation, regulatory proteins, and upstream promoter elements. The text also describes protein coding genes of higher eukaryotes; instability of messenger RNA in bacteria; and replication control of the ColE1-type plasmids. The text then describes translation initiation, including the translation of prokaryotes and eukaryotes. The book puts emphasis on the selective degradation of abnormal proteins in bacteria. Topics include proteins rapidly hydrolyzed in E. coli; intracellular aggregates of abnormal polypeptides; energy requirement and pathway for proteins; proteolytic enzymes in E. coli; and regulation of ion expression. The text also highlights the detection of proteins produced by recombinant DNA techniques and mechanism and practice. The book is a good source of information for readers wanting to study gene expression.



Maximizing Gene Expression


Maximizing Gene Expression
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Author : Reznikoff William
language : en
Publisher:
Release Date : 1986

Maximizing Gene Expression written by Reznikoff William and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1986 with categories.




Principles Of Gene Manipulation


Principles Of Gene Manipulation
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Author : R. W. Old
language : en
Publisher: Univ of California Press
Release Date : 1981

Principles Of Gene Manipulation written by R. W. Old and has been published by Univ of California Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1981 with Science categories.




Molecular Biology And Biotechnology


Molecular Biology And Biotechnology
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Author : Robert Allen Meyers
language : en
Publisher: John Wiley & Sons
Release Date : 1995-06-29

Molecular Biology And Biotechnology written by Robert Allen Meyers and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-06-29 with Medical categories.


This is one volume 'library' of information on molecular biology, molecular medicine, and the theory and techniques for understanding, modifying, manipulating, expressing, and synthesizing biological molecules, conformations, and aggregates. The purpose is to assist the expanding number of scientists entering molecular biology research and biotechnology applications from diverse backgrounds, including biology and medicine, as well as physics, chemistry, mathematics, and engineering.



Guide To Protein Purification


Guide To Protein Purification
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Author : Murray P. Deutscher
language : en
Publisher: Gulf Professional Publishing
Release Date : 1990

Guide To Protein Purification written by Murray P. Deutscher and has been published by Gulf Professional Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with Proteins categories.


Guide to Protein Purification , designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features* Detailed procedures newly written for this volume* Extensive practical information* Rationale and strategies for protein and enzyme purification* Personal perspectives on enzyme purification by eminent researchersAmong the Topics Covered* General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structure.



Post Transcriptional Control Of Gene Expression


Post Transcriptional Control Of Gene Expression
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Author : Orna Resnekov
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29

Post Transcriptional Control Of Gene Expression written by Orna Resnekov and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.


Many important cellular processes rely on posttranscriptional control of gene expression. This book describes the mechanisms of gene expression at this level that occur in the cytoplasm of prokaryotes and eukaryotes. Several introductory chapters discuss the general principles of translation and mRNA stability. The interactions of mature mRNA with the translational machinery, the components of mRNA degradation and antisense RNA are surveyed. Subsequent chapters discuss protein folding, transport, modification and degradation. The book is an invaluable source of information for both newcomers and those wishing an overview of the field.



Post Transcriptional Control Of Gene Expression


Post Transcriptional Control Of Gene Expression
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Author : John E.G. McCarthy
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29

Post Transcriptional Control Of Gene Expression written by John E.G. McCarthy and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.


The last ten years have witnessed a remarkable increase in our awareness of the importance of events subsequent to transcriptional initiation in terms of the regulation and control of gene expression. In particular, the development of recombinant DNA techniques that began in the 1970s provided powerful new tools with which to study the molecular basis of control and regulation at all levels. The resulting investigations revealed a diversity of post-transcriptional mechanisms in both prokaryotes and eukaryotes. Scientists working on translation, mRNA stability, transcriptional (anti)termination or other aspects of gene expression will often have met at specialist meetings for their own research area. However, only rarely do workers in different areas of post-transcriptional control/ regulation have the opportunity to meet under one roof. We therefore thought it was time to bring together leading representatives of most of the relevant areas in a small workshop intended to encourage interaction across the usual borders of research, both in terms of the processes studied, and with respect to the evolutionary division prokaryotes/eukaryotes. Given the breadth of topics covered and the restrictions in size imposed by the NATO workshop format, it was an extraordinarily difficult task to choose the participants. However, we regarded this first attempt as an experiment on a small scale, intended to explore the possibilities of a meeting of this kind. Judging by the response of the participants during and after the workshop, the effort had been worthwhile.



Recent Advancements In Gene Expression And Enabling Technologies In Crop Plants


Recent Advancements In Gene Expression And Enabling Technologies In Crop Plants
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Author : Kasi Azhakanandam
language : en
Publisher: Springer
Release Date : 2015-03-03

Recent Advancements In Gene Expression And Enabling Technologies In Crop Plants written by Kasi Azhakanandam and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-03-03 with Science categories.


In this book, authors who are experts in their fields describe current advances on commercial crops and key enabling technologies that will underpin future advances in biotechnology. They discuss state of the art discoveries as well as future challenges. Tremendous progress has been made in introducing novel genes and traits into plant genomes since the first creation of transgenic plants thirty years ago, and the first commercialization of genetically modified maize in 1996. Consequently, cultivation of biotech crops with useful traits has increased more than 100-fold from 1.7 million hectares in 1996 to over 175 million hectares globally in 2013. This achievement has been made possible by continued advances in understanding the basic molecular biology of regulatory sequences to modulate gene expression, enhancement of protein synthesis and new technologies for transformation of crop plants. This book has three sections that encompass knowledge on genetically modified (GM) food crops that are currently used by consumers, those that are anticipated to reach the market place in the near future and enabling technologies that will facilitate the development of next generation GM crops. Section I focuses only on genetically modified maize and soybean (3 chapters each), while Section II discusses the GM food crops rice, wheat, sorghum, vegetables and sugar cane. Section III covers exciting recent developments in several novel enabling technologies, including gene targeting, minichromosomes, and in planta transient expression systems.



Recombinant Gene Expression


Recombinant Gene Expression
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Author : Paulina Balbas
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04

Recombinant Gene Expression written by Paulina Balbas and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.


Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on—gene expression. Although at the lower end of the complexity scale, this microbe became a very successful model system and a key player in the fantastic revolution kindled by the birth of recombinant DNA technology.



Molecular Genetic And Statistical Techniques For Behavioral And Neural Research


Molecular Genetic And Statistical Techniques For Behavioral And Neural Research
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Author : Robert T. Gerlai
language : en
Publisher: Academic Press
Release Date : 2018-04-24

Molecular Genetic And Statistical Techniques For Behavioral And Neural Research written by Robert T. Gerlai and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-24 with Science categories.


Molecular-Genetic and Statistical Techniques for Behavioral and Neural Research presents the most exciting molecular and recombinant DNA techniques used in the analysis of brain function and behavior, a critical piece of the puzzle for clinicians, scientists, course instructors and advanced undergraduate and graduate students. Chapters examine neuroinformatics, genetic and neurobehavioral databases and data mining, also providing an analysis of natural genetic variation and principles and applications of forward (mutagenesis) and reverse genetics (gene targeting). In addition, the book discusses gene expression and its role in brain function and behavior, along with ethical issues in the use of animals in genetics testing. Written and edited by leading international experts, this book provides a clear presentation of the frontiers of basic research as well as translationally relevant techniques that are used by neurobehavioral geneticists. - Focuses on new techniques, including electrocorticography, functional mapping, stereo EEG, motor evoked potentials, optical coherence tomography, magnetoencephalography, laser evoked potentials, transmagnetic stimulation, and motor evoked potentials - Presents the most exciting molecular and recombinant DNA techniques used in the analysis of brain function and behavior - Written and edited by leading international experts