Membrane Protein Expression Systems
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Membrane Protein Expression Systems
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Author : Gwyn W. Gould
language : en
Publisher: Ashgate Publishing
Release Date : 1994
Membrane Protein Expression Systems written by Gwyn W. Gould and has been published by Ashgate Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Science categories.
The use of heterologous expression as a means to study membrane proteins is becoming widespread. This book is a simple to read laboratory manual describing the principles behind the commonly used expression systems, eg baculovirus, oocytes, cell culture systems and yeast.
Heterologous Expression Of Membrane Proteins
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Author : Isabelle Mus-Veteau
language : en
Publisher: Springer Nature
Release Date : 2022-06-30
Heterologous Expression Of Membrane Proteins written by Isabelle Mus-Veteau and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-06-30 with Science categories.
This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.
Production Of Membrane Proteins
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Author : Anne Skaja Robinson
language : en
Publisher: John Wiley & Sons
Release Date : 2011-06-15
Production Of Membrane Proteins written by Anne Skaja Robinson and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-15 with Science categories.
Designed as a research-level guide to current strategies and methods of membrane protein production on the small to intermediate scale, this practice-oriented book provides detailed, step-by-step laboratory protocols as well as an explanation of the principles behind each method, together with a discussion of its relative advantages and disadvantages. Following an introductory section on current challenges in membrane protein production, the book goes on to look at expression systems, emerging methods and approaches, and protein specific considerations. Case studies illustrate how to select or sample the optimal production system for any desired membrane protein, saving both time and money on the laboratory as well as the technical production scale. Unique in its coverage of "difficult" proteins with large membrane-embedded domains, proteins from extremophiles, peripheral membrane proteins, and protein fragments.
Cell Free Protein Expression
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Author : W. Antoni Kudlicki
language : en
Publisher: CRC Press
Release Date : 2007-11-27
Cell Free Protein Expression written by W. Antoni Kudlicki and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-27 with Science categories.
Following its inception in the 1950s, cell-free protein synthesis made a tremendous impact on the basic life sciences. The use of cell-free systems was key to understanding molecular mechanisms underlying one of the most complicated processes found in nature: protein translation. Since this time, aggressive cutting-edge research and stiff commerica
Membrane Protein Protocols
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Author : Barry S. Selinsky
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Membrane Protein Protocols written by Barry S. Selinsky and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.
Expression Purification And Structural Biology Of Membrane Proteins
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Author : Camilo Perez
language : en
Publisher: Humana
Release Date : 2020-02-29
Expression Purification And Structural Biology Of Membrane Proteins written by Camilo Perez and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-02-29 with Science categories.
This book collects up-to-date advanced protocols and advice from leading experts in the area of membrane protein biology that can be applied to structural and functional studies of any membrane protein system. The contents explore methods for cloning and expression of membrane proteins and membrane protein complexes in prokaryotic and eukaryotic systems, approaches for protein purification, nanobody applications, as well as biophysical characterization and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Expression, Purification, and Structure Biology of Membrane Proteins serves to guide and encourage young researchers and newcomers to the field to tackle bold new studies on membrane proteins. Chapter 11 is available open access under a CC-BY 4.0 license via link.springer.com.
Structural Genomics On Membrane Proteins
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Author : Kenneth H. Lundstrom
language : en
Publisher: CRC Press
Release Date : 2006-02-23
Structural Genomics On Membrane Proteins written by Kenneth H. Lundstrom and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-02-23 with Medical categories.
While the genomic revolution has quickly led to the deposit of more than 30,000 structures in the protein data bank (PDB), less than one percent of those contributions represent membrane proteins despite the fact that membrane proteins constitute some 20 percent of all proteins. This discrepancy becomes significantly troublesome when it is coupled
Structural Biology Of Membrane Proteins
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Author : Reinhard Grisshammer
language : en
Publisher: Royal Society of Chemistry
Release Date : 2007-10-31
Structural Biology Of Membrane Proteins written by Reinhard Grisshammer and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-31 with Science categories.
In the last few years there have been many exciting and innovative developments in the field of membrane protein structure and this trend is set to continue. Structural Biology of Membrane Proteins is a new monograph covering a wide range of topics with contributions from leading experts in the field. The book is split into three sections: the first discusses topics such as expression, purification and crystallisation; the second covers characterisation techniques and the final section looks at new protein structures. The book will hence have wide appeal to researchers working in and around the field and provide an up-to-date reference source. Introductory sections to each topic are accompanied by more detailed discussions for the more experienced biochemist. Detailed descriptions of experimental methods are included to demonstrate practical approaches to membrane protein structure projects. The book also offers an up-to-date reference source in addition to descriptions of new and emerging developments, including state-of-the-art techniques for solving membrane protein structures. Structural Biology of Membrane Proteins encompasses both basic introductions and detailed descriptions of themes and should appeal to a wide range of biochemical scientists, both experienced and beginner.
Membrane Proteins Production And Functional Characterization
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Author :
language : en
Publisher: Academic Press
Release Date : 2015-04-06
Membrane Proteins Production And Functional Characterization written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-04-06 with Science categories.
Membrane Proteins – Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines. Volume of Methods In Enzymology Contains a collection of a diverse array of topics in the area of membrane protein biology ranging from recombinant expression, isolation, functional characterization, biophysical studies and crystallization
Membrane Proteins Production For Structural Analysis
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Author : Isabelle Mus-Veteau
language : en
Publisher: Springer
Release Date : 2014-06-20
Membrane Proteins Production For Structural Analysis written by Isabelle Mus-Veteau and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-06-20 with Science categories.
This book updates the latest development in production, stabilization and structural analysis techniques of membrane proteins. This field has made significant advances since the elucidation of the first 3-D structure of a recombinant G Protein Coupled Receptor (GPCR), rhodopsin, with the structure of several more GPCRs having been solved in the past five years. In fact, the 2012 Nobel Prize in Chemistry was awarded for groundbreaking discoveries on the inner workings of GPCRs. This book is essential reading for all researchers, biochemists and crystallographers working with membrane proteins, who are interested by the structural characterization of their favorite protein and who wish to follow the expression, migration, modifications and recycling of a membrane protein.