Methods For Affinity Based Separations Of Enzymes And Proteins
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Methods For Affinity Based Separations Of Enzymes And Proteins
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Author : Munishwar N. Gupta
language : en
Publisher: Birkhäuser
Release Date : 2013-12-01
Methods For Affinity Based Separations Of Enzymes And Proteins written by Munishwar N. Gupta and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-01 with Science categories.
One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase extractions, expanded bed chromatography, perfusion chromatography and monoliths) described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors.
Handbook Of Methods And Instrumentation In Separation Science
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Author :
language : en
Publisher: Academic Press
Release Date : 2009-11-11
Handbook Of Methods And Instrumentation In Separation Science written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-11-11 with Science categories.
Handbook of Methods and Instrumentation in Separation Science, Volume 1 provides concise overviews and summaries of the main methods used for separation. It is based on the Encyclopedia of Separation Science. The handbook focuses on the principles of methods and instrumentation. It provides general concepts concerning the subject matter; it does not present specific procedures. This volume discusses the separation processes including affinity methods, analytical ultracentrifugation, centrifugation, chromatography, and use of decanter centrifuge and dye. Each methodology is defined and compared with other separation processes. It also provides specific techniques, principles, and theories concerning each process. Furthermore, the handbook presents the applications, benefits, and validation of the processes described in this book. This handbook is an excellent reference for biomedical researchers, environmental and production chemists, flavor and fragrance technologists, food and beverage technologists, academic and industrial librarians, and nuclear researchers. Students and novices will also find this handbook useful for practice and learning. - One-stop source for information on separation methods - General overviews for quick orientation - Ease of use for finding results fast - Expert coverage of major separation methods - Coverage of techniques for all sizes of samples, pico-level to kilo-level
Enzymes In The Valorization Of Waste
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Author : Pradeep Verma
language : en
Publisher: CRC Press
Release Date : 2022-12-19
Enzymes In The Valorization Of Waste written by Pradeep Verma and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-12-19 with Science categories.
Enzymes in the Valorization of Waste: Next-Gen Technological Advances for Sustainable Development of Enzyme-based Biorefinery focusses on key enzymes which are involved in the development of integrated biorefinery. It highlights the modern next-gen technologies for promoting the application of sustainable and greener enzymatic steps at industrial scale for the development of futuristic and self-sustainable "consolidated/integrated biorefinery/enzyme-based biorefinery." It also deals with technological advancement for improvement of enzyme yield or specificity, conversion capability, such as protein and metabolic engineering and advances in next generation technologies, and so forth. Features: • Explores all modern-day technologies that can potentially be used in enzyme-based biorefinery conversion of wastes to value-added products. • Covers technological, economic, and environmental assessments of enzyme-based biorefinery prospects. • Deliberates all possible products that can be generated from wastes including biofuel and essential chemicals. • Illustrates techniques for enhanced yield and properties to be used in various industrial applications. • Reviews advanced information of relevant sources and mechanism of enzymes. This book is aimed at graduate students, researchers and related industry professionals in biochemical engineering, environmental science, wastewater treatment, biotechnology, applied microbiology, biomass-based biorefinery, biochemistry, green chemistry, sustainable development, waste treatment, enzymology, microbial biotechnology, and waste valorization.
The Three Functional States Of Proteins
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Author : Timir Tripathi
language : en
Publisher: Elsevier
Release Date : 2024-11-17
The Three Functional States Of Proteins written by Timir Tripathi and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-11-17 with Science categories.
The Three Functional States of Proteins explores how structured proteins, intrinsically disordered proteins, and phase separated proteins contribute to the complexity of cellular life, and offers insights into their roles in both health and disease. It discusses the latest research findings and highlight groundbreaking discoveries and innovative methodologies used to study these protein states.Traditionally, the different states of proteins have been defined based on their structures and functions. However, it is becoming increasingly clear that these criteria alone may not be sufficient to capture the complex and multifaceted properties of these molecules. Definitions based on thermodynamics and kinetics are now recognized as potentially more appropriate for comprehensively understanding protein states. Emerging evidence indicates that under physiological conditions, a majority of proteins possess the capability to exist in and transition between the native, droplet, and amyloid states. These distinct states play crucial roles in various cellular functions, influenced significantly by their physicochemical and structural properties. The book also considers the interactions among these states and discusses how their internal organization as individual molecules, as well as their collective organization as molecular assemblies are stabilized. Furthermore, it examines the processes by which these states are formed and the cellular functions associated with each specific state. - The book serves as an introduction to a unique volume that provides comprehensive coverage of these three functional states of proteins - The chapters are written by leading global scientists who are actively engaged in research on these specific protein states - It presents a broad picture of the current, emerging, and evolving research on these protein states - Given that this book comprehensively addresses both foundational concepts and recent advancements in the field, it will appeal a broad spectrum of readers from various academic disciplines
Peptides
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Author : Norbert Sewald
language : en
Publisher: John Wiley & Sons
Release Date : 2015-11-19
Peptides written by Norbert Sewald and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-11-19 with Science categories.
Der "Sewald/Jakubke" ist und bleibt das einzige moderne, auf dem neuesten wissenschaftlichen Stand gehaltene fortgeschrittene Lehrbuch der Peptid-Biochemie! Auch für diese zweite, um ein Viertel erweitere Auflage haben die Autoren Hunderte von Publikationen gelesen und zu einem informativen und vollständigen Überblick über das Fachgebiet zusammengefasst. Nach einer Einführung in die Grundlagen der Peptidstruktur und -synthese werden die wichtigsten Familien biologisch aktiver Peptide systematisch abgehandelt. Mit interdisziplinärem Anspruch werden dann wichtige Anwendungen aus der Biotechnologie, Pharmazie und Biomedizin besprochen. Selbstverständlich werden auch Themen der Spitzenforschung berücksichtigt, zum Beispiel Pseudopeptide, Peptidmimetika und Aspekte der kombinatorischen Synthese. -- Neu in dieser Auflage sind Fragen und Übungsaufgaben, die das Nacharbeiten von Vorlesungen oder das Selbststudium erleichtern.
Separation Extraction And Concentration Processes In The Food Beverage And Nutraceutical Industries
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Author : Syed S. H. Rizvi
language : en
Publisher: Elsevier
Release Date : 2010-10-28
Separation Extraction And Concentration Processes In The Food Beverage And Nutraceutical Industries written by Syed S. H. Rizvi and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-10-28 with Technology & Engineering categories.
Separation, extraction and concentration are essential processes in the preparation of key food ingredients. They play a vital role in the quality optimization of common foods and beverages and there is also increasing interest in their use for the production of high-value compounds, such as bioactive peptides from milk and whey, and the recovery of co-products from food processing wastes.Part one describes the latest advances in separation, extraction and concentration techniques, including supercritical fluid extraction, process chromatography and membrane technologies. It also reviews emerging techniques of particular interest, such as pervaporation and pressurised liquid extraction. Part two then focuses on advances in separation technologies and their applications in various sectors of the food, beverage and nutraceutical industries. Areas covered include dairy and egg processing, oilseed extraction, and brewing. This section discusses the characteristics of different foods and fluids, how food constituents are affected by separation processes and how separation processes can be designed and operated to optimize end product quality.With its team of experienced international contributors, Separation, extraction and concentration processes in the food, beverage and nutraceutical industries is an important reference source for professionals concerned with the development and optimisation of these processes. - Describes the latest advances in separation, extraction and concentration techniques and their applications in various sectors of the food, beverage and nutraceutical industries - Reviews emerging techniques of particular interest, such as pervaporation and pressurised liquid extraction - Explores the characteristics of different foods and fluids and how food constituents are affected by separation processes
Handbook Of Affinity Chromatography
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Author : Toni Kline
language : en
Publisher: CRC Press
Release Date : 1993-06-16
Handbook Of Affinity Chromatography written by Toni Kline and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-06-16 with Science categories.
Outlining the fundamental principles by which all interactions occur, this reference focuses on harnessing the biochemistry of bioorganic compounds in order to separate them, presenting new techniques and applications that affect the planning of research strategies. The contributors discuss how to c
Microbial Enzymes As Potential Biotherapeutics In Human Healthcare
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Author : Dileep Francis
language : en
Publisher: CRC Press
Release Date : 2025-08-04
Microbial Enzymes As Potential Biotherapeutics In Human Healthcare written by Dileep Francis and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-08-04 with Science categories.
This book comprehensively reviews the diverse potential of microbial enzymes with therapeutic applications, covering their production, purification, and utilization. It examines their roles in combating various diseases, including inflammation, bacterial infections, cardiovascular conditions, and thrombotic disorders. The book highlights their pharmaceutical applications in drug formulation and bioactive compound production, with the aim of enhancing drug bioavailability, stability, and efficacy. Furthermore, the book explores the potential of microbial fibrinolytic enzymes to revolutionize treatments for thrombotic disorders. It also outlines the applications of microbial enzymes in targeting cancer cells, emphasizing their pivotal role in innovative treatment modalities. Additionally, the book presents the therapeutic applications of microbial lipases, from their use in lipid-lowering medications to the creation of lipase-based pharmaceuticals. The chapters discuss the potential of microbial uricases in managing uric-acid-related disorders and the contribution of microbial enzymes in enhancing digestion and alleviating digestive disorders. Finally, the book delves into the vital role of regulatory authorities and intellectual property rights (IPR) in the commercialization of therapeutic microbial enzymes. As such, this book serves as a valuable resource for researchers, professionals, and students of microbiology, biotechnology, and pharmaceutical sciences. Key Features: Reviews the therapeutic potential of microbial enzymes and their production and purification Examines the roles of microbial enzymes in combating a wide range of diseases, including inflammation, bacterial infections, cardiovascular conditions, and thrombotic disorders Highlights the pharmaceutical applications of microbial enzymes, focusing on drug formulation and the production of bioactive compounds to enhance drug bioavailability, stability, and efficacy Explores the therapeutic applications of microbial lipases, from lipid-lowering medications to the creation of lipase-based pharmaceuticals Presents the vital role of regulatory authorities and intellectual property rights (IPR) in the commercialization of therapeutic microbial enzymes
Advances In Chromatography
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Author : Nelu Grinberg
language : en
Publisher: CRC Press
Release Date : 2021-12-19
Advances In Chromatography written by Nelu Grinberg and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-12-19 with Science categories.
For six decades, scientists and researchers have relied on the Advances in Chromatography series for the most up-to date information on a wide range of developments in chromatographic methods and applications. The clear presentation of topics and vivid illustrations for which this series has become known makes the material accessible and engaging to analytical, biochemical, organic, polymer, and pharmaceutical chemists at all levels of technical skill. Key Features: Discusses the basic concepts of affinity chromatography and examines recent developments in this method and related supramolecular separation methods. Outlines the different types of gradient stationary phases and how they have been used in and benefited the field of separation science. Reviews recent trends in detectors for GC, focusing on those that are readily available and seeing wide usage. Addresses peak compression in GELC and offers the reader a plate height equation to work with that incorporates its effects.
Immobilization Of Enzymes And Cells
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Author : José M. Guisán
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Immobilization Of Enzymes And Cells written by José M. Guisán and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation—from the laboratory to industry—of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of protocols for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few protocols are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related properties.