Methods For Affinity Based Separations Of Enzymes And Proteins
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Methods For Affinity Based Separations Of Enzymes And Proteins
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Author : Munishwar N. Gupta
language : en
Publisher: Birkhäuser
Release Date : 2013-12-01
Methods For Affinity Based Separations Of Enzymes And Proteins written by Munishwar N. Gupta and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-01 with Science categories.
One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase extractions, expanded bed chromatography, perfusion chromatography and monoliths) described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors.
Encyclopedia Of Separation Science
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Author : Colin F. Poole
language : en
Publisher: Academic Press
Release Date : 2000-09-06
Encyclopedia Of Separation Science written by Colin F. Poole and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-09-06 with Science categories.
The Encyclopedia of Separation Science is the most comprehensive resource available on the theory, techniques, and applications of separation science. The work presents information on three levels. The first volume contains Level 1, which provides a broad overview of the theory of the 12 main categories of separation techniques. Volumes 2-4 (Level 2) expand coverage with detailed theoretical and technical descriptions of particular techniques. The remaining Volumes 5-9 (Level 3) cover applications of these techniques from the micro to the macro, and also from the analytical laboratory bench to large-scale industrial processes. Volume 10 consists mainly of the index. Initial access to the online version offering extensive hypertext linking and advanced search tools is available with purchase. Ongoing access is maintained for a minimum annual fee. The Encyclopedia of Separation Science is the first truly comprehensive work covering the whole of separation theory, methods, and techniques. This encyclopedia will be invaluable to researchers and professionals across a wide range of areas in academia and industry. Enclyclopedia of Separation Science is available online via ScienceDirect offering enhanced features such as extensive cross-referencing and dynamic linking. For more information please (http://www.info.sciencedirect.com/reference_works/works_available/separation/index.shtml click here.)
Affinity Membranes
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Author : Elias Klein
language : en
Publisher: Wiley-Interscience
Release Date : 1991-02-14
Affinity Membranes written by Elias Klein and has been published by Wiley-Interscience this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991-02-14 with Science categories.
Explores the latest findings for both selective and efficient separation devices in the field of kidney research. It is divided into three major sections. Part one deals with the ``biochemistry'' part of the problem, including how to identify ligands of interest, how to link them to synthetic membranes, and some kinetic limitations of frontal elution chromatography. The second part comprehensively discusses the various substrata used in affinity separations and the formation processes of semi-permeable membranes. The final section explores the filtration processes using membranes and the kinetics of separations based on affinity membranes.
Protein Purification
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Author : R.K. Scopes
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
Protein Purification written by R.K. Scopes and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.
New textbooks at all levels of chemistry appear with great regularity. Some fields like basic biochemistry, organic reaction mechanisms, and chemical ther modynamics are well represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the grad uate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research which is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In some cases the availability of texts in active research areas should help stimulate the creation of new courses. New York CHARLES R.
Downstream Processing Of Proteins
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Author : Mohamed A. Desai
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Downstream Processing Of Proteins written by Mohamed A. Desai and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
Considerable effort and time is allocated to introducing cell culture and fermentation technology to undergraduate students in academia, generally through a range of courses in industrial biotechnology and related disciplines. Similarly, a large number of textbooks are available to describe the appli- tions of these technologies in industry. However, there has been a general lack of appreciation of the significant developments in downstream processing and isolation technology, the need for which is largely driven by the stringent re- latory requirements for purity and quality of injectable biopharmaceuticals. This is particularly reflected by the general absence of coverage of this s- ject in many biotechnology and related courses in educational institutions. For a considerable while I have felt that there is increasing need for an introductory text to various aspects of downstream processing, particularly with respect to the needs of the biopharmaceutical and biotechnology ind- try. Although there are numerous texts that cover various aspects of protein purification techniques in isolation, there is a need for a work that covers the broad range of isolation technology in an industrial setting. It is anticipated that Downstream Processing of Proteins: Methods and Protocols will play a small part in filling this gap and thus prove a useful contribution to the field. It is also designed to encourage educational strategists to broaden the coverage of these topics in industrial biotechnology courses by including accounts of this important and rapidly developing element of the industrial process.
Biocatalysis For Practitioners
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Author : Gonzalo de Gonzalo
language : en
Publisher: John Wiley & Sons
Release Date : 2021-07-19
Biocatalysis For Practitioners written by Gonzalo de Gonzalo and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-07-19 with Science categories.
This reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.
Liquid Chromatography
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Author : Salvatore Fanali
language : en
Publisher: Elsevier
Release Date : 2017-06-23
Liquid Chromatography written by Salvatore Fanali and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-06-23 with Science categories.
Liquid Chromatography: Applications, Second Edition,is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their knowledge of the wide variety of applications in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. - Emphasizes the integration of chromatographic methods and sample preparation - Explains how liquid chromatography is used in different industrial sectors - Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) - Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making
Guide To Protein Purification
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Author : Richard R Burgess
language : en
Publisher: Academic Press
Release Date : 2009-11-03
Guide To Protein Purification written by Richard R Burgess and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-11-03 with Science categories.
Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. - Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology - Assembles chapters on both common and less common relevant techniques - Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process
Immobilized Affinity Ligand Techniques
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Author : Greg T. Hermanson
language : en
Publisher: Academic Press
Release Date : 1992-08-27
Immobilized Affinity Ligand Techniques written by Greg T. Hermanson and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992-08-27 with Science categories.
This book is a practical guide to the preparation and use of immobilized affinity ligands for purification, catalysis, and analysis. Special emphasis is given to immunochemical techniques including antibody isolation, preparation of antibody fragments using immobilized enzymes, and immunoaffinity chromatography. The book provides easy-to-follow, well-tested protocols to allow the uninitiated to use these techniques to the maximum advantage with minimum hassle. In addition, it shows researchers how to save money by making their own optimized affinity supports. Matrix activation: Ligand immobilization, Binding and elution of target molecules, Enzyme catalysis on solid supports, Analytical affinity chromatography, Isolation/purification of antibodies, Preparation of antibody fragments, Immunoaffinity chromatography, Immobilization of nucleic acids, Use of immobilized ligands for removal of trace contaminants Practical advice on choosing: Matrices, Spacers, Methods of activation and coupling Background information and insights on: Affinity interactions, The ease and power of affinity chromatography, Attaching molecules to insoluble supports, Matrices currently in use, Over 20 methods of activation, Spacers, Extensive References
Applied Biophysics For Drug Discovery
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Author : Donald Huddler
language : en
Publisher: John Wiley & Sons
Release Date : 2017-10-02
Applied Biophysics For Drug Discovery written by Donald Huddler and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-10-02 with Science categories.
Applied Biophysics for Drug Discovery is a guide to new techniques and approaches to identifying and characterizing small molecules in early drug discovery. Biophysical methods are reasserting their utility in drug discovery and through a combination of the rise of fragment-based drug discovery and an increased focus on more nuanced characterisation of small molecule binding, these methods are playing an increasing role in discovery campaigns. This text emphasizes practical considerations for selecting and deploying core biophysical method, including but not limited to ITC, SPR, and both ligand-detected and protein-detected NMR. Topics covered include: • Design considerations in biophysical-based lead screening • Thermodynamic characterization of protein-compound interactions • Characterizing targets and screening reagents with HDX-MS • Microscale thermophoresis methods (MST) • Screening with Weak Affinity Chromatography • Methods to assess compound residence time • 1D-NMR methods for hit identification • Protein-based NMR methods for SAR development • Industry case studies integrating multiple biophysical methods This text is ideal for academic investigators and industry scientists planning hit characterization campaigns or designing and optimizing screening strategies.