Methods For Analysis Of Golgi Complex Function
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Methods For Analysis Of Golgi Complex Function
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Author :
language : en
Publisher: Elsevier
Release Date : 2013-11-26
Methods For Analysis Of Golgi Complex Function written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-26 with Science categories.
This new volume of Methods in Cell Biology looks at methods for analyzing of golgi complex function. Chapters cover such topics as in vitro reconstitution systems, fluorescence-based analysis of trafficking in mammalian cells and high content screening. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material
Golgi
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Author : Yanzhuang Wang
language : en
Publisher: Springer Nature
Release Date : 2022-12-13
Golgi written by Yanzhuang Wang and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-12-13 with Science categories.
This volume provides readers with a collection of new and classical methods, techniques, and applications used to address enduring questions about the structure and functions of the Golgi complex. The chapters in this volume cover diverse topics ranging from model systems; live and fixed cell imaging techniques; in vitro biochemical reconstitution systems; and specific methods developed to study Golgi formation, maintenance, and functions under physiological and pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Golgi: Methods and Protocols is a valuable tool for researchers in the field who wish to explore new areas of Golgi biology and for new investigators interested in exploring Golgi structure and function.
The Golgi Apparatus
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Author : James Morré
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-12-11
The Golgi Apparatus written by James Morré and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-12-11 with Science categories.
The Golgi apparatus (GA) is typically comprised of a series of five to eight cup-shaped, membrane-covered sacs called cisternae that look something like a stack of deflated balloons. The GA is often considered the "distribution and shipping department" for the cell's chemical products. This book traces the first 100 years of GA discovery from the first published accounts from Pavia, Italy, in 1898 to the Centenary Celebration in Pavia, Italy, in 1998 to our most recent discoveries. It summarizes the past 50 years beginning with the modern era of GA discovery, leading up to the present era with almost exclusive focus on molecular biology.
The Golgi Apparatus
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Author : Eric Berger
language : en
Publisher: Birkhäuser
Release Date : 2012-12-06
The Golgi Apparatus written by Eric Berger and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
In 1898 Camillo Golgi reported his newly observed intracellular structure, the apparato reticolare interno, now universally known as the Golgi Apparatus. The method he used was an ingenious histological technique (La reazione nera) which brought him fame for the discovery of neuronal networks and culminated in the award of the Nobel Prize for Physiology and Medicine in 1906. This technique, however, was not easily reproducible and led to a long-lasting controversy about the reality of the Golgi apparatus. Its identification as a ubiquitous organelle by electron microscopy turned out to be the breakthrough and incited an enormous wave of interest in this organelle at the end of the sixties. In recent years immunochemical techniques and molecular cloning approaches opened up new avenues and led to an ongoing resurgence of interest. The role of the Golgi apparatus in modifying, broadening and refining the structural information conferred by transcription/translation is now generally accepted but still incompletely understood. During the coming years, this topic certainly will remain center stage in the field of cell biology. The centennial of the discovery of this fascinating organelle prompted us to edit a new comprehensive book on the Golgi apparatus whose complexity necessitated the contributions of leading specialists in this field. This book is aimed at a broad readership of glycobiologists as well as cell and molecular biologists and may also be interesting for advanced students of biology and life sciences.
Golgi Apparatus
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Author : Christopher J. Hawkins
language : en
Publisher:
Release Date : 2011
Golgi Apparatus written by Christopher J. Hawkins and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with Golgi apparatus categories.
The Golgi apparatus is an organelle found in most eukaryotic cells. The primary function of the Golgi apparatus is to process and package macromolecules, such as proteins and lipids, after their synthesis and before they make their way to their destination. This book presents topical research data in the study of Golgi apparatus, including Golgi organization and stress sensing; signaling pathways controlling mitotic Golgi breakdown in mammalian cells; the role of Golgi apparatus in the biological mechanisms of hypericin-mediated photodynamic therapy; the role of the Trans-Golgi Network (TGN) in the sorting of nonenzymic lysosomal proteins; and the mechanisms involving the role of Golgi apparatus alteration in neurological disorders triggered by manganese.
Analysis Of Golgi Apparatus During Germ Cell Differentiation And Formation And Function Of The Hermes Body Of Epididymal Sperm
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Author : Elliot Byrne
language : en
Publisher:
Release Date : 2015
Analysis Of Golgi Apparatus During Germ Cell Differentiation And Formation And Function Of The Hermes Body Of Epididymal Sperm written by Elliot Byrne and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
"Introduction: At the last step of spermiogenesis, Oko et al. (1993) proposed that unstacked cisternae of the Golgi ribbon organized into a structure previously known as the cytoplasmic droplet, here referred to as the Hermes body. Conserved among all mammalian species, the migration of the Hermes body along the midpiece of sperm in the epididymis is observed to coincide with the initiation of sperm motility. The objective of this study was to compare the most abundant protein constituents of isolated Hermes bodies of epididymal sperm as well as Golgi apparatuses of germ cells in order to provide insight into the formation and function of the Hermes body as well as to ascertain the patterns of expression and differentiation of several Golgi markers during spermatogenesis. Methods: 58 antibodies were selected for immunohistochemical localization (IHC) done on sections of adult male rat testes and epididymides (n=4). Reaction patterns were visualized in all germ cell types across the 14 stages of spermatogenesis, in epididymal sperm throughout the epididymal duct, as well as in all somatic cell types in order to establish each protein's pattern of expression.Results: Each of the 58 antibodies analyzed in this study was investigated as a molecular map of protein expression through the 14 stages of spermatogenesis and for their presence in the Hermes body. Quantitatively, the most abundant Hermes body proteins were those for glucose transport (Glut3) and metabolism (Hexokinase 1), each was localized to the Hermes body by IHC. Actin and myosin were identified to propel the Hermes along the flagellum. In addition, the Hermes body contained proteins characteristic of ER, endosomes, and Golgi apparatus both by proteomics as well as by IHC. In the testis Golgi fraction, the transmembrane 9 family of proteins were prominent and of unknown function along with 200 other proteins. The most abundant protein of this fraction was a novel putative glycosyl transferase (GL54D), an unkown protein but verified by IHC in situ. Golgi proteins conserved between the Hermes and the testis germ cell Golgi apparatus and verified by IHC included the TMED family of transmembrane proteins and COPI/II coatomer proteins. Golgi proteins were also revealed during mitosis, meiosis, acrosome and postacrosomal formation, and Hermes body formation, establishing a protein signature for each during germ cell differentiation. Of the 43 proteins localized to the Hermes body of epididymal sperm, only 31 were maintained down the entire duct, which is explained by the presence of members of the ubiquitin-proteasome system. Discussion: The results of this study reveal that although noted in the Hermes body, Golgi apparatus proteins were not abundant in forming and epididymal Hermes bodies. Hence, the flattened cisternae of the Hermes body may represent a hybrid structure that has evolved to suit the specific needs of the sperm during its maturation.Conclusions: The Hermes body is deduced to compartmentalize glycolysis, cytoskeletal, membrane trafficking, stress response, and protein degradation for the initiation of sperm motility and sperm maturation in the epididymis with its complete protein compendium realized in the step 19 spermatid during Hermes body formation. The Golgi apparatus of germ cells also demonstrates a specific complement of proteins that would serve for the differentiation of germ cells during spermatogenesis." --
The Golgi Complex Methods And Protocols
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Author : William J. Brown
language : en
Publisher: Methods in Molecular Biology
Release Date : 2018-10-07
The Golgi Complex Methods And Protocols written by William J. Brown and has been published by Methods in Molecular Biology this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-07 with Science categories.
Focusing on new methods and techniques developed to address longstanding questions about the Golgi complex, this volume explores a diverse set of chapters, ranging from live and fixed cell imaging techniques toin vitro biochemical reconstitution systems. Each chapter provides a detailed set of specific instructions, which should enable anyone to successfully complete the assays. Written for the highly successful Methods in Molecular Biology series program, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips from the experts on troubleshooting and avoiding known pitfalls. Practical and detailed, The Golgi Complex: Methods and Protocols will aid both new and established researchers in the field by providing strong hands-on instructions that can be directly applied to their research programs.iv>
The Golgi Apparatus
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Author : Alexander A. Mironov
language : en
Publisher: Springer
Release Date : 2009-08-29
The Golgi Apparatus written by Alexander A. Mironov and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-08-29 with Science categories.
This book summarizes all new data obtained after development of methods of Golgi complex sub fractionation, molecular biology and microscopy. It collects the full range of expertise, different points of view and different approaches. The book is devoted to molecular modes of the function of the Golgi apparatus as a whole, taking into account all experimental data. The book aims to make the functional organization of the Golgi apparatus more understandable.
The Golgi Apparatus
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Author : Alexander A. Mironov
language : en
Publisher: Springer
Release Date : 2008-09-04
The Golgi Apparatus written by Alexander A. Mironov and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-09-04 with Science categories.
This book summarizes all new data obtained after development of methods of Golgi complex sub fractionation, molecular biology and microscopy. It collects the full range of expertise, different points of view and different approaches. The book is devoted to molecular modes of the function of the Golgi apparatus as a whole, taking into account all experimental data. The book aims to make the functional organization of the Golgi apparatus more understandable.
Molecular Biology Of The Cell
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Author : Bruce Alberts
language : en
Publisher:
Release Date : 2002
Molecular Biology Of The Cell written by Bruce Alberts and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Cytology categories.