Methods In Cellular Immunology Second Edition

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Methods In Cellular Immunology

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Author : Rafael Fernandez-Botran
language : en
Publisher: CRC Press
Release Date : 2001-06-26

Methods In Cellular Immunology written by Rafael Fernandez-Botran and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-06-26 with Medical categories.

A step-by-step guide to commonly used procedures, Methods in Cellular Immunology addresses both human and murine models, in addition to such topics as PCR and apoptosis. The basic format of the original version has been maintained, and the goal remains the same: to make it a useful and easy-to-use tool for investigators employing cellular immunolog

Methods In Cellular Immunology Second Edition

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Author : Rafael Fernandez-Botran
language : en
Publisher: CRC Press
Release Date : 2001-06-26

Methods In Cellular Immunology Second Edition written by Rafael Fernandez-Botran and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-06-26 with Medical categories.

A step-by-step guide to commonly used procedures, Methods in Cellular Immunology addresses both human and murine models, in addition to such topics as PCR and apoptosis. The basic format of the original version has been maintained, and the goal remains the same: to make it a useful and easy-to-use tool for investigators employing cellular immunological techniques in their research, regardless of whether or not immunology is their main area of expertise. It provides information about manufacturers and commercial sources of chemicals and reagents and a comprehensive list of references, allowing readers to refer back to the original information and/or techniques.

Differential Display Methods And Protocols

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Author : Peng Liang
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04

Differential Display Methods And Protocols written by Peng Liang and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.

Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression. The gene-hunting euphoria was initially powered by the invention of DD, which was gradually overtaken by DNA microarray technology in recent years. Then why is there still the need for second edition of this DD book? First of all, DD still enjoys a substantial lead over DNA microarrays in the ISI citation data (see Table 1), despite the h- dreds of millions of dollars spent each year on arrays. This may come as a surprise to many, but to us it implies that many of the DNA microarray studies went unpublished owing to their unfulfilled promises (1). Second, unlike DNA microarrays, DD is an “open”-ended gene discovery method that does not depend on prior genome sequence information of the organism being studied. As such, DD is applicable to the study of all living organisms—from bacteria, fungi, insects, fish, plants, to mammals—even when their genomes are not sequenced. Second, DD is more accessible technically and financially to most cost-conscious “cottage-industry” academic laboratories. So clearly DD still has its unique place in the modern molecular biological toolbox for gene expression analysis.

Ion Channels

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Author : James D. Stockand
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04

Ion Channels written by James D. Stockand and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.

Internationally recognized biomedical scientists describe recent technological breakthroughs and demonstrate their use in successful experimental designs. The diverse applications range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, for studying channel regulation and physiological function, and for genetic screening and investigating channelopathies.

Transmembrane Signaling Protocols

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Author : Hydar Ali
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05

Transmembrane Signaling Protocols written by Hydar Ali and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.

The previous edition of Transmembrane Signaling Protocols was published in 1998. Since then the human genome has been completely sequenced and new methods have been developed for the use of microarrays and proteomics to analyze global changes in gene expression and protein profiles. These advances have increased our ability to understand transmembrane signaling processes in much greater detail. They have also simultaneously enhanced our ability to determine the role of a large number of newly identified molecules in signaling events. In addition, novel video microscopy methods have been developed to image transmembrane signaling events in live cells in real time. In view of these major advances, it is time to update the previous edition. Because of the success of that volume, we have chosen to keep the essential character of the book intact. Introductory chapters from experts have been included to provide overall perspective and an overview of recent advances in signal transduction pathways. The individual chapters now include comp- hensive detailed methods, studies in genetically tractable systems, fluorescence microscopy in live single cells, ex vivo analysis of primary cells from tra- genic mice, as well as genomic and proteomic approaches to the analysis of transmembrane signaling events. We would like to express our deep gratitude to the coauthors of this publi- tion. We hope that Transmembrane Signaling Protocols, Second Edition will serve as a valuable resource for future progress in the study of signal transd- tion pathways.

Epidermal Growth Factor

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Author : Tarun B. Patel
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05

Epidermal Growth Factor written by Tarun B. Patel and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.

A comprehensive collection of optimized methods for dissecting the mechanisms that control epidermal growth factors (EGF) and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Clinical Applications Of Pcr

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Author : Y. M. Dennis Lo
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04

Clinical Applications Of Pcr written by Y. M. Dennis Lo and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.

In this updated second edition, leading researchers apply molecular diagnostics to the many recent advances that have occurred in polymerase chain reaction( PCR)-based technologies. Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis.

Microchip Capillary Electrophoresis

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Author : Charles Henry
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04

Microchip Capillary Electrophoresis written by Charles Henry and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.

Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.

Protein Design

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Author : Raphael Guerois
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04

Protein Design written by Raphael Guerois and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.

Proteins have evolved through selective pressure to accomplish specific functions. The functional properties of proteins depend upon their thr- dimensional structures, which result from particular amino acid sequences folding into tightly packed domains. Thus, to understand and modulate protein function rationally, one definitely needs methods and algorithms to predict and decipher how amino acid sequences shape three-dimensional structures. Protein design aims precisely at providing the tools to achieve this goal. The predictive power of rational protein design methods has dramatically increased over the past five years. A broad range of studies now illustrate how the sequence of proteins and peptides can be tuned to engineer biological tools with intended properties (1–3). The extensive characterization of peptides and protein mutants has enormously benefited the understanding of protein sequence-to-structure relationships. Synergies between computational and experimental approaches have also added momentum to the advancing limits of design methods. The potential applications in fundamental biochemistry and in biotechnology justify the considerable excitement that this progress has generated within the research community. The field is probably mature enough so that expert knowledge can assist researchers of diverse disciplines to rationally create or modify their favorite protein. Thus, the aim of Protein Design: Methods and Protocols is to account for the most up-to-date protein design and engineering strategies so that readers can undertake their own projects with maximum confidence in a successful return. The basic concepts underlying rational design of proteins are intimately related to their three-dimensional structures.

Diagnostic Bacteriology Protocols

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Author : Louise O’Connor
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05

Diagnostic Bacteriology Protocols written by Louise O’Connor and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.

The field of bacterial diagnostics has seen unprecedented advances in recent years. The increased need for accurate detection and identification of bacteria in human, animal, food, and environmental samples has fueled the development of new techniques. The field has seen extensive research aided by the information from bacterial genome sequencing projects. Although traditional methods of bacterial detection and identification remain in use in laboratories around the world, there is now a growing trend toward the use of nucleic ac- based diagnostics and alternative biochemically and immunologically based formats. The ultimate goal of all diagnostic tests is the accurate detection, identification, or typing of microorganisms in samples of interest. Although the resulting information is of obvious use in the areas of patient management, animal health, and quality control, it is also of use in monitoring routes of infection and outlining strategies for infection control. There is, therefore, a need to ensure that the information being provided is of the highest standard and that any new technique is capable of delivering this.