Methods In Immunology
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Physical And Chemical Methods
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Author : Curtis A. Williams
language : en
Publisher: Academic Press
Release Date : 2014-05-12
Physical And Chemical Methods written by Curtis A. Williams and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-12 with Medical categories.
Methods in Immunology: Volume II, Physical and Chemical Methods is a collection of papers dealing with electrophoresis, analytical ultracentrifugation, dialysis, ultrafiltration, cellulose ion exchangers, and chromatographic separation of macromolecules on porous gels. Some papers explain the applications of radioisotopes, optical analysis, and chemical analysis of proteins, carbohydrates, lipids, and nucleic acid. One paper describes the theory of electro-migration. Factors such as electrical charge or frictional coefficients govern the rate of migration of charged particles in an electric field. The differences found in their velocities can be used to separate substances or analyze them. Mobility is a characteristic property of molecules and can also be influenced by the composition of the medium or solution. Dialysis separates solvents too large to diffuse through a barrier from smaller solutes; ultrafiltration (reverse osmosis) forces solvent and solutes up to a certain critical size through the barrier by a high pressure on one side. The book notes that the membrane never becomes plugged in dialysis because of some opposite movement of the solvent. Another paper points out that the significance of radioactive tracers in immunochemistry employed to identify and label macromolecules functioning as antigens and antibodies. The collection can prove valuable to bio-chemists, cellular biologists, micro-biologists, developmental biologists, and scientists involved in immunological research.
Methods In Immunology
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Author : Justine S. Garvey
language : en
Publisher:
Release Date : 1983
Methods In Immunology written by Justine S. Garvey and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983 with Immunology categories.
Laboratory Methods In Immunology
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Author : Heddy Zola
language : en
Publisher: CRC Press
Release Date : 1990-05-23
Laboratory Methods In Immunology written by Heddy Zola and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-05-23 with Medical categories.
This two-volume reference details immunological techniques for biologists of all disciplines. Volume I includes a detailed discussion of the tissue culture laboratory. It addresses what the lab needs to be, and the general "housekeeping" procedures involved in tissue culture. Presented next are chapters on specific aspects of tissue culture and hybridoma technology. The book includes a review of bioassays for interleukins, and a series of papers on lymphokines and functional assays in vitro. The section on molecular genetic studies begins with consideration of the choice of strategies for cloning the genes of cell surface molecules. It continues with papers on aspects of molecular biological techniques most closely related to immunology. The final section covers immunochemical techniques. Volume II reviews techniques used with small laboratory animals. It includes papers on specialized procedures with animals. Technical aspects are emphasized through a detailed analysis of effects of ultraviolet light on the immune system. Covered also is antigen detection in cells and tissue. The book addresses the important areas of protein purification using monoclonal antibodies.These two volumes are of great importance to those who use immunological techniques, whether they are immunologists or trained in other disciplines. The book is intended for those in animal science, veterinary science, genetics and cell biology, bacteriology, immunology, pathology, biochemistry, laboratory medicine, and hematology.
Immunological Methods In Microbiology
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Author :
language : en
Publisher: Academic Press
Release Date : 2020-05-13
Immunological Methods In Microbiology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-05-13 with Science categories.
Immunological Methods in Microbiology, Volume 47 in the Methods in Microbiology series, highlights new advances in the field, with this new volume presenting interesting chapters on Immunological Techniques in the Clinical laboratory, Immunologic Diagnosis of HIV and Opportunistic Infections, Combining Antigen Detection and Serology for the Diagnosis of Selected Infectious Diseases, Immunologic Detection of Lyme Disease and Related Borrelioses, Immunodetection of Bacteria Causing Brucellosis, Immunological Diagnostic Techniques Used to Identify and Type Pasteurella, Immunological Tests for Diarrhea caused by Diarrheagenic Escherichia coli Targeting Their Main Virulence Factors, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Microbiology series Includes the latest information on Immunological Methods in Microbiology
Mathematical Methods In Immunology
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Author : Jerome Kenneth Percus
language : en
Publisher: American Mathematical Soc.
Release Date : 2012
Mathematical Methods In Immunology written by Jerome Kenneth Percus and has been published by American Mathematical Soc. this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Mathematics categories.
Any organism, to survive, must use a variety of defense mechanisms. A relatively recent evolutionary development is that of the adaptive immune system, carried to a quite sophisticated level by mammals. The complexity of this system calls for its encapsulation by mathematical models, and this book aims at the associated description and analysis. In the process, it introduces tools that should be in the armory of any current or aspiring applied mathematician, in the context of, arguably, the most effective system nature has devised to protect an organism from its manifold invisible enemies.
Methods In Immunology
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Author : Dan Hampston Campbell
language : en
Publisher:
Release Date : 1970
Methods In Immunology written by Dan Hampston Campbell and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1970 with Immunity categories.
Methods In Immunology
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Author : Dan Hampton Campbell
language : en
Publisher:
Release Date : 1970
Methods In Immunology written by Dan Hampton Campbell and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1970 with Immunity categories.
Methods In Immunology
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Author : Dan Hampton Campbell
language : en
Publisher:
Release Date : 1964
Methods In Immunology written by Dan Hampton Campbell and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1964 with Immunology categories.
Immunological Methods
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Author : Ivan Lefkovits
language : en
Publisher: Academic Press
Release Date : 2014-06-28
Immunological Methods written by Ivan Lefkovits and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-06-28 with Science categories.
Immunological Methods a compendium of basic research techniques being used in one of the largest immunology research institutes, the Basel Institute for Immunology, with particular emphasis given to new methodology. The procedures have been described by individuals judged to be highly expert in their specialties. In many instances the methods developed or adapted to unique uses by the contributors have not previously been described in detail. The book contains 34 chapters covering techniques for detection, isolation, and purification of antibodies (including dansylation, two-dimensional chromatography, isoelectric focusing, polyacrylamide gel electrophoresis, and isotachophoresis); measurement of equilibrium constants (equilibrium dialysis, filtration, and sedimentation); and isotope and fluorescent labeling and detection of cell-surface components. Techniques such as isotope laboratory maintenance; chemical modification of proteins, haptens, and solid supports, and haptenation of viable biological carriers; production of antisera against allotypes and histocompatibility antigens and production of antibody with clonai dominance; histocompatibility and MLR testing; and cell separation by haptenated gels and by velocity sedimentation of rosette-forming cells are also discussed. Other chapters cover detection of antibody-secreting and alloantigen-binding cells; immune responses in vitro and their analysis by limiting dilution; production of T-cell factors; hybridoma production by cell fusion; maintenance of cell lines and cloning in semisolid media; and the mathematical analysis of immunological data.
Immunological Methods
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Author : Ivan Lefkovits
language : en
Publisher: Elsevier
Release Date : 2014-05-12
Immunological Methods written by Ivan Lefkovits and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-12 with Nature categories.
Immunological Methods, Volume III provides information pertinent to the fundamental aspects of immunological methods. This book presents recombinant DNA technology as applied to immunology. Organized into 25 chapters, this volume begins with an overview of the major histocompatibility complex. This text then examines the use of monoclonal antibodies in the identification of lymphocyte membrane antigens and explains the use of monoclonal antibodies in histocompatibility typing. Other chapters consider the methods in two-dimensional electrophoretic analysis of proteins and another to lymphokines that support B cells. This book discusses as well the production and expansion of cloned B and T cell lines and hybridomas. The final chapter deals with the sophisticated methodology used with particular animal species, namely, birds, sheep, and amphibians to probe specific immunological questions. This book is a valuable resource for immunologists and investigators with extensive experience in using immunological methods. Protein chemists and biologists will also find this book useful.