Methods In Membrane Biology
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Methods In Membrane Biology
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Author : Edward D. Korn
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-04-17
Methods In Membrane Biology written by Edward D. Korn and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-17 with Science categories.
Less than a year before this writing, a Nobel Prize was shared by Albert Claude, Christian de Duve, and George Palade, pioneers in the development of modern cell biology, of which membrane biology is an integral part. For many years, a seemingly unbridgeable gap separated the physiologist working at the organ level from the biochemist studying the molecular composition of cell constituents and the chemical reactions that occur in water-soluble extracts of cells. Physiology has a long history, and the disciplines epitomized by intermediary metabolism and molecular biology progressed rapidly during the 1950s and 1960s. Meanwhile, electron micros copists painstakingly mapped the newly discovered intracellular world of membranes, organelles, microtubules, and microfilaments, and other scien tists developed techniques for the quantitative separation and characteriza tion of these intracellular structures. Thus it finally became possible to localize the many enzymes, and the metabolic activities they catalyze, to recognizable structures whose composition and organization can be studied. We are now well on our way to bridging that gap between biochemistry and physiology-to understanding how the cell functions.
Methods In Membrane Biology
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Author : Edward Korn
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Methods In Membrane Biology written by Edward Korn and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Many of the methods now in general use in membrane biology, and not already discussed in satisfactory detail elsewhere, have been covered in the eight previously published volumes of this series. Much of this ninth volume is occupied by one authoritative chapter, an unusually thorough and critical review of a relatively new and highly specialized technology that has gained rapid acceptance: immunofluorescence and immunoelectron microscopy. These are powerful experimental tools applicable in fields much broader than membrane research and employing methods drawn from widely diverse disciplines such as organic chemistry, protein chemistry, immunology, and fluorescence and electron microscopy. The temptation to use these super ficially, and deceptively, simple but fundamentally complex methods un critically is almost overwhelming. The chapter by de Petris, a pioneer in the field, is as necessary as it is rigorous, and it should long be the standard in this area of research. The second chapter in this volume is a more specialized review by Matus of the procedures for the preparation and characterization of the highly differentiated junctional regions of brain plasma membranes. These methods are central to the rapidly growing field of neurobiochemistry membrane biochemistry at perhaps its most intricate.
An Introduction To Biological Membranes
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Author : William Stillwell
language : en
Publisher: Newnes
Release Date : 2013-04-20
An Introduction To Biological Membranes written by William Stillwell and has been published by Newnes this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-20 with Science categories.
An Introduction to Biological Membranes: From Bilayers to Rafts covers many aspects of membrane structure/function that bridges membrane biophysics and cell biology. Offering cohesive, foundational information, this publication is valuable for advanced undergraduate students, graduate students and membranologists who seek a broad overview of membrane science. - Brings together different facets of membrane research in a universally understandable manner - Emphasis on the historical development of the field - Topics include membrane sugars, membrane models, membrane isolation methods, and membrane transport
Methods In Membrane Biology
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Author : Edward D. Korn
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Methods In Membrane Biology written by Edward D. Korn and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The purposes of this senes were discussed in the preface to Volume I: to present "a range of methods . . . from the physical to the physiological . . . in sufficient detail for the reader to use them in his laboratory" and also to describe "the theoretical backgrounds of the methods and their limita tions in membrane biology" so that the reader will be enabled "to evaluate more critically and to understand more fully data obtained by methods foreign to [his] usual experiences. " The chapter by Lee, Birdsall, and Metcalfe with which Volume 2 begins accomplishes these twin goals with a thorough description of the application of nuclear magnetic relaxation measurements to membrane biology together with a lucid and succinct integration of the results of such studies into present concepts of the organi zation of membrane lipids. This then permits speculation on the physical basis of membrane permeability. The powerful tool of NMR spectroscopy will have even fuller application with the development of techniques, al ready partially exploited, for l3C-Iabeling of specific carbon atoms in lipid molecules and with extension of the observations to membrane proteins. The following two chapters, by Glick and by Laine, Stellner, and Hako mori, describe the isolation and characterization of membrane glycoproteins and membrane glycolipids, respectively.
Methods In Membrane Biology
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Author : Edward D. Korn
language : en
Publisher:
Release Date : 1974
Methods In Membrane Biology written by Edward D. Korn and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1974 with Cytology categories.
Methods In Membrane Biology
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Author : Edward D. Korn
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Methods In Membrane Biology written by Edward D. Korn and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
Examination of the tables of contents of journals - biochemical, molecular biological, ultrastructural, and physiological-provides convincing evidence that membrane biology will be in the 1970s what biochemical genetics was in the 1960s. And for good reason. If genetics is the mechanism for main taining and transmitting the essentials of life, membranes are in many ways the essence of life. The minimal requirement for independent existence is the individualism provided by the separation of "life" from the environment. The cell exists by virtue of its surface membran~. One might define the first living organism as that stage of evolution where macromolecular catalysts or self-reproducing polymers were first segregated from the surrounding milieu by a membrane. Whether that early membrane resembled present cell membranes is irrelevant. What matters is that a membrane would have provided a mechanism for maintaining a local concentration of molecules, facilitating chemical evolution and allowing it to evolve into biochemical evolution. That or yet more primitive membranes, such as a hydrocarbon monolayer at an air-water interface, could also have provided a surface that would facilitate the aggregation and specific orientation of molecules and catalyze their interactions. If primitive membranes were much more than mere passive barriers to free diffusion, how much more is this true of the membranes of contemporary forms of life. A major revolution in biological thought has been the recogni tion that the cell, and especially the eukaryotic cell, is a bewildering maze of membranes and membranous organelles.
Membrane Structural Biology
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Author : Mary Luckey
language : en
Publisher: Cambridge University Press
Release Date : 2008-03-17
Membrane Structural Biology written by Mary Luckey and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-03-17 with Science categories.
Cutting-edge text providing a foundation for membrane biology suitable for advanced students and working scientists.
Methods In Membrane Biology
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Author : Edward D. Korn
language : en
Publisher:
Release Date : 2014-09-01
Methods In Membrane Biology written by Edward D. Korn and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-09-01 with categories.
Molecular Biology Of The Cell
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Author :
language : en
Publisher:
Release Date : 2004
Molecular Biology Of The Cell written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Cells categories.
Methods In Membrane Biology
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Author : Edward D. Korn
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Methods In Membrane Biology written by Edward D. Korn and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Although not the only volume in this series in which lipids are discussed, the present volume is devoted entirely to methods for the study of membrane lipids. Even now, when membrane proteins are properly receiving so much attention, this emphasis on membrane lipids is appropriate. Essentially all of the phospholipids and sterols of cells are in membranes. Moreover, although membrane proteins are certainly of utmost importance, the more we learn about the functional properties of membrane proteins, the more we appreciate the unique features of phospholipids, without which biological membranes would be impossible. The hydrophobic-hydrophilic duality of phospholipids allows, indeed requires, their association, in an aqueous environment, into an essentially two-dimensional membrane-only molec ularly thick in one dimension but relatively infinite in the other two; a structure composed of small molecules, not covalently linked, and therefore, infinitely mobile and variable, but yet a structure with great stability and one largely impermeable to most biomolecules. These membrane-forming properties are shared by many amphipathic polar lipids-phospholipids, glycolipids, and sphingolipids-that differ significantly from each other in the nature of their polar head groups and their fatty acids. These variations in structure allow a range of specific interactions among membrane lipids and between lipids and proteins and also provide for membranes of variable, but controlled, fluidity. In this way, phospholipids provide an appropriate milieu for functional membrane proteins and also significantly modulate their catalytic activities.