Methods In Molecular Biology Protocols In Human Molecular Genetics
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Methods In Molecular Biology Protocols In Human Molecular Genetics
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Author : John M. Walker
language : en
Publisher:
Release Date : 1984
Methods In Molecular Biology Protocols In Human Molecular Genetics written by John M. Walker and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols categories.
Protocols In Human Molecular Genetics
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Author : Christopher G. Mathew
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Protocols In Human Molecular Genetics written by Christopher G. Mathew and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Medical categories.
Extraordinary advances have been made in the field of human molecular genetics during the past five years. The ability to amplify a specific region ofDNA a millionfold in a few hours using the polymerase chain reaction has led to the rapid identification of mutations in human disease and of DNA sequence polymorphisms on every human chro- some. DNA fragments of up to 1 megabase in length can now be resolved by pulsed-field gel electrophoresis to create long-range physical maps of important regions of the genome, and can be cloned in the form of yeast artificial chromosomes. The discovery of highly variable "minisatellite" DNA sequences has led to the development of DNA fingerprinting. The application of these techniques to the study of the human genome has culminated in major advances such as the cloning of the cystic fibrosis gene, the construction of genetic linkage maps of each human chro- some, the mapping of many genes responsible for human inherited d- orders, genetic fingerprinting of forensic specimens, and the detection of mutations involved in the development of human tumors. Although many of the new techniques in molecular genetics can be learned relatively easily, it is often difficult for a researcher to obtain all of the relevant information necessary for getting up a technique and applying it successfully. The information available in the research lite- ture often lacks the depth of description that the new user requires.
Pichia Protocols
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Author : James M Cregg
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-08-08
Pichia Protocols written by James M Cregg and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-08-08 with Science categories.
This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.
Cell Biology Protocols
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Author : J. Robin Harris
language : en
Publisher:
Release Date : 2006-03-06
Cell Biology Protocols written by J. Robin Harris and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-03-06 with Science categories.
Cell biology involves a range of techniques for examining how cells function, regulate their own behavior, and interact with their neighbors. This book, the first in a series of five comprehensive methods handbooks, covers key protocols in this dynamic field including cellular organelles, cell growth and division, cell movement, cell signaling, and cell death. Because molecular biology approaches are widely used in cell biology, a few essential techniques from that field are also included.
Techniques In Molecular Medicine
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Author : Friedhelm Hildebrandt
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Techniques In Molecular Medicine written by Friedhelm Hildebrandt and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Medical categories.
Standard molecular biology techniques are used widely in biomedical re search and have had a major impact on the understanding of physiologic processes and disease mechanisms. They also play an increasing role in mo lecular genetic diagnosis and new therapeutic approaches. This laboratory manual is designed for researchers who need reliable pro tocols for their laboratorywork. It provides a step by step approach to stan dard methods of molecular biology. A wide range of topics is covered by protocols for immediate application in the laboratory. These include pur ification, isolation, labeling, hybridization, sequencing and enzymatic mod ification ofDNA or RNA, polymerase chain reaction, cloning, libraries, and protein techniques. The manual is based on experience with a practical course in molecular biologytechniques, which was taught bythe authors. Since most methods in molecular biology recapitulate in a test tube mechanisms that occur natu rally in the cell, the first three chapters describe and illustrate basic mechan isms of molecular biology. U nderstanding more about these basic mechan isms will enable the researcher to apply techniques more specifically, based on the knowledge of their potential and limits. W e hope that users find this manual a helpful guide on their road to exciting insights.
Chemical Biology
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Author : Jonathan E. Hempel
language : en
Publisher: Humana
Release Date : 2016-09-24
Chemical Biology written by Jonathan E. Hempel and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-09-24 with Science categories.
This volume seeks to enable the discovery of tools in chemical biology by providing readers with various techniques ranging from initial chemical genetic screening to target identification. To successfully highlight the essential components of the chemical biology tool discovery process, the book is organizes into four parts that focus on platforms for molecular discovery in in vitro cellular systems, in vivo chemical genetic screening protocols, and methods used to discover functional protein targets. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and informative, Chemical Biology: Methods and Protocols seeks to improve the success rate of the chemical biology field through the dissemination of detailed and experiential knowledge.
Statistical Human Genetics
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Author : Robert C. Elston
language : en
Publisher: Humana Press
Release Date : 2012-02-04
Statistical Human Genetics written by Robert C. Elston and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-02-04 with Science categories.
Recent advances in genetics over the last quarter of a century, especially in molecular techniques, have dramatically reduced the cost of determining genetic markers and hence opened up a field of research that is increasingly helping to detect, prevent and/or cure many diseases that afflict humans. In Statistical Human Genetics: Methods and Protocols expert researchers in the field describe statistical methods and computer programs in the detail necessary to make them more easily accessible to the beginner analyzing data. Written in the highly successful Methods in Molecular BiologyTM series format, with examples of running the programs and interpreting the program outputs, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results from human genetic data collected in the laboratory. Thorough and as much as possible intuitive, Statistical Human Genetics: Methods and Protocols aids scientists in understanding the computer programs and analytical procedures they need to use.
Genetic Epidemiology
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Author : Evangelos Evangelou
language : en
Publisher: Humana Press
Release Date : 2018-06-07
Genetic Epidemiology written by Evangelos Evangelou and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-06-07 with Medical categories.
This volume details fast-moving research while providing in-depth descriptions of methods and analytical approaches that are helping to understand the genome and how it is related to complex diseases. Chapters guide the reader through common and rare variation, gene-gene and gene-environment interactions and state-of-the-art approaches for the synthesis of genome-wide and gene expression data. Novel approaches for associations in the HLA region, family-based designs, Mendelian Randomization and Copy Number Variation are also presented. The volume concludes with the challenges researchers face while moving from identifying variants to their functional role and potential drug targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, a thorough presentation of methods and approaches and tips on troubleshooting and avoiding known pitfalls.
Genotyping
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Author : Stefan J. White
language : en
Publisher: Humana
Release Date : 2016-11-08
Genotyping written by Stefan J. White and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-11-08 with Medical categories.
This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping.
Rna
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Author : Henrik Nielsen
language : en
Publisher: Humana Press
Release Date : 2010-12-10
Rna written by Henrik Nielsen and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-12-10 with Medical categories.
Recent insight into the transcripts generated from the mammalian genome (i.e. the transcriptome) has revealed that transcription is a far more complex phenomenon than previously thought. In RNA: Methods and Protocols, expert researchers provide the procedures and methods used to describe the structure of messenger RNAs and non-coding RNAs that are transcribed by RNA polymerase II as the immediate gene products in mammalian cells. Focused on the structure of the RNA products of “gene X” and the mapping of proteins associated with these RNAs, the volume presents appropriate information for non-specialists in RNA biology. Written in the highly successful Methods in Molecular BiologyTM series format, many chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, RNA: Methods and Protocols views the transcriptional landscape with an appreciation for the role that proteins play in the processing and interpretation of genetic information in an attempt to further our crucial knowledge of the many products and sophisticated regulatory networks that result from it.