Methods In Molecular Biology Volume 136

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Methods In Molecular Biology Volume 136

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Author : Rocky S. Tuan
language : en
Publisher:
Release Date : 2000

Methods In Molecular Biology Volume 136 written by Rocky S. Tuan and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with categories.

Developmental Biology Protocols

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Author : Rocky S. Tuan
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05

Developmental Biology Protocols written by Rocky S. Tuan and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.

Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the org- ism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and mole- lar biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced - portant paradigms for both basic and clinical biomedical sciences alike. Although modern developmental biology has its roots in “experimental emb- ology” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

The Kidney

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Author : Peter D. Vize
language : en
Publisher: Elsevier
Release Date : 2003-03-14

The Kidney written by Peter D. Vize and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-03-14 with Science categories.

Organogenesis of the kidney has been intensely studied for over a century. In recent years advances in molecular techniques have not only made great inroads into exploring the genetic regulation of this complex process but also began to unravel the molecular basis of many forms of congenital kidney disease. This book is a comprehensive study on these findings and the only book available with such in depth coverage of the kidney. - Hundreds of color figures depicting key events in all aspects of kidney development - Full coverage of the genetic and cellular basis of kidney development - Analysis of the genetic basis of the major congenital kidney diseases

Yac Protocols

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Author : Alasdair MacKenzie
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-26

Yac Protocols written by Alasdair MacKenzie and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-26 with Science categories.

It is now 10 years since the first edition of YAC Protocols was published in 1996. YAC Protocols was first produced to address the huge demand within the research community for a lab-based text that described in detail the wide range of uses for large insert yeast artificial chromosome (YAC) DNA clones. In doing this, the original editor, David Markie, and the many different contri- tors who provided descriptions of the protocols they used and developed, did a magnificent job. Indeed many of the techniques described within the first e- tion require little change and have stood up admirably to the test of time. Since the first edition, the use of YACs has proved invaluable for addressing a wide range of new biological problems ranging from those of basic biochemistry to assisting in the mapping and sequencing of the human genome. The requirement for a second edition of YAC Protocols was prompted by a number of major advances in biology since the publication of the first edition. These advances have included the sequencing of the human genome, and the genomes of a wide variety of other organisms, and the increased use of transgenic animals for understanding the molecular basis of human and animal disease.

Gene Therapy Methods

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Author : M. Ian Phillips
language : en
Publisher: Academic Press
Release Date : 2002-02-22

Gene Therapy Methods written by M. Ian Phillips and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-02-22 with Medical categories.

This volume in the prestigious Methods in Enzymology series discusses methods currently used in preclinical and clinical gene therapy. Subjects covered in this book, such as the use of adeno-associated virus delivery for treatment of Parkinson's disease, are topical and are presented in the methods-oriented style popularized by this series. Discusses methods currently used in preclinical and clinical gene therapy Covers the use of adeno-associated virus delivery for treatment of Parkinson's disease

Sterile Insect Technique

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Author : Victor A. Dyck
language : en
Publisher: CRC Press
Release Date : 2021-01-06

Sterile Insect Technique written by Victor A. Dyck and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-01-06 with Science categories.

The sterile insect technique (SIT) is an environment-friendly method of pest control that integrates well into area-wide integrated pest management (AW-IPM) programmes. This book takes a generic, thematic, comprehensive, and global approach in describing the principles and practice of the SIT. The strengths and weaknesses, and successes and failures, of the SIT are evaluated openly and fairly from a scientific perspective. The SIT is applicable to some major pests of plant-, animal-, and human-health importance, and criteria are provided to guide in the selection of pests appropriate for the SIT. In the second edition, all aspects of the SIT have been updated and the content considerably expanded. A great variety of subjects is covered, from the history of the SIT to improved prospects for its future application. The major chapters discuss the principles and technical components of applying sterile insects. The four main strategic options in using the SIT — suppression, containment, prevention, and eradication — with examples of each option are described in detail. Other chapters deal with supportive technologies, economic, environmental, and management considerations, and the socio-economic impact of AW-IPM programmes that integrate the SIT. In addition, this second edition includes six new chapters covering the latest developments in the technology: managing pathogens in insect mass-rearing, using symbionts and modern molecular technologies in support of the SIT, applying post-factory nutritional, hormonal, and semiochemical treatments, applying the SIT to eradicate outbreaks of invasive pests, and using the SIT against mosquito vectors of disease. This book will be useful reading for students in animal-, human-, and plant-health courses. The in-depth reviews of all aspects of the SIT and its integration into AW-IPM programmes, complete with extensive lists of scientific references, will be of great value to researchers, teachers, animal-, human-, and plant-health practitioners, and policy makers.

Methods In Protein Design

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Author :
language : en
Publisher: Academic Press
Release Date : 2013-02-14

Methods In Protein Design written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-02-14 with Science categories.

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers methods in protein design and it has chapters on such topics as protein switch engineering by domain insertion, evolution based design of proteins, and computationally designed proteins. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers methods in protein design - Contains chapters with such topics as protein switch engineering by domain insertion, evolution-based design of proteins, and computationally designed proteins

Methods In Methane Metabolism

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Author : Amy Claire Rosenzweig
language : en
Publisher: Academic Press
Release Date : 2011

Methods In Methane Metabolism written by Amy Claire Rosenzweig and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with Enzymology categories.

Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state of the art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field.

Guide To Techniques In Mouse Development Part B

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Author : Paul Wassarman
language : en
Publisher: Academic Press
Release Date : 2010-09-24

Guide To Techniques In Mouse Development Part B written by Paul Wassarman and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-09-24 with Science categories.

This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development - Update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993 - Comprehensively covers: - new techniques for the cryopreservation of gametes and embryos - production of transgenic and null (knockout) animals (use of ES cells) - generation of conditional/inducible mutant animals, use of gene-trap mutagenesis - analysis of allele-specific expression, use of new reporter constructs - humanizing of transgenic animals - transcript profiling of mouse development - imaging of mouse development - rederivation of animals and use of mouse genomics

Guide To Techniques In Mouse Development Part A

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Author :
language : en
Publisher: Academic Press
Release Date : 2010-08-04

Guide To Techniques In Mouse Development Part A written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-08-04 with Science categories.

Guide to Techniques in Mouse Development, Part A comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. - Update of volume 225 of Methods in Enzymology, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993 - Covers new technologies and methodologies, including: - new techniques for the cryopreservation of gametes and embryos - production of transgenic and null (knockout) animals (use of ES cells) - generation of conditional/inducible mutant animals - use of gene-trap mutagenesis - analysis of allele-specific expresion - use of new reporter constructs - humanizing of transgenic animals - transcript profiling of mouse development - imaging of mouse development - rederivation of animals and use of mouse genomics