Microarrays
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Microarrays
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Author : Kilian Dill
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-12-30
Microarrays written by Kilian Dill and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-12-30 with Science categories.
Combinatorial chemistry is used to find materials that form sensor microarrays. This book discusses the fundamentals, and then proceeds to the many applications of microarrays, from measuring gene expression (DNA microarrays) to protein-protein interactions, peptide chemistry, carbodhydrate chemistry, electrochemical detection, and microfluidics.
Dna Microarrays
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Author : Mark Schena
language : en
Publisher: Practical Approach Series
Release Date : 1999
Dna Microarrays written by Mark Schena and has been published by Practical Approach Series this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Medical categories.
DNA microarrays, or biochips, are small glass chips embedded with ordered rows of DNA and by providing a massive parallel platform for data gathering represent a fundamental technical advance in biomedical research. Such biochips enable the use of advanced fabrication, detection, and data mining technologies that allow data gathering at an unprecedented rate. The first chapter is an introduction to the technology of DNA microarrays, emphasizing the methodological fundamentals of biochips. The next two chapters describe the use of confocal scanning in microarray detection and techniques for the efficient cloning and screening of differentially expressed genes. Chapter 4 describes assay optimization for enzymatic assays and chapter 5 antisense scanning arrays. This is followed by a chapter on the manufacture of molecular arrays using ink-jet printing technology. Chapter 7 describes gene expression analysis from cDNA microarrays and then chapter 8 covers the use of expression data in bioinformatics. Chapter 9 describes the use of active microelectronic arrays for DNA hybridization analysis and the last chapter details the use of microarray technology in pharmacogenomics. This Practical Approach book is a comprehensive overview of the new and expanding field of DNA microarray technology and will be invaluable to any researcher interested in the use of biochips.
Methods In Microarray Normalization
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Author : Phillip Stafford
language : en
Publisher: CRC Press
Release Date : 2008-01-31
Methods In Microarray Normalization written by Phillip Stafford and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-01-31 with Mathematics categories.
This organized text compiles, for the first time, the most useful normalization methods developed for interpreting microarray data. Experts examine the mathematical processes that are important in normalizing data and avoiding inherent systematic biases. They also review modern software, including discussions on key algorithms, comparative data, and download locations. The book contains the latest microarray innovations from companies such as Agilent, Affymetrix, and GeneGo as well as new, readily adaptable normalization methods for expression and CGH arrays. It also lists of open-source molecular profiling normalization algorithms available and where to access them.
Microarrays
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Author : Jang B. Rampal
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-07-27
Microarrays written by Jang B. Rampal and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-07-27 with Medical categories.
Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and protocols for the preparation of microarrays. The second volume details applications and data analysis, which is important in analyzing the enormous data coming out of microarray experiments. Among the topics discussed in Volume 1: Synthesis Methods, are matrices in the synthesis of microarrays, array optimization processes, array-based comparative genomic hybridization, 60-mer oligonucleotide probes, bifunctional reagents NTMTA and NTPAC, and high density arrays using digital microarray synthesis platforms. Other topics include multiplex ligation-dependent probe amplification (MLPA), hybridization conditions in situ-synthesized oligo arrays, peptide arrays, high density replication tools (HDRT), protocols for the quantification of oligo hybridization, glyco-bead arrays, and an investigation into the emerging nano technology. Microarray Technology, Volumes 1 and 2, provide ample information to all levels of scientists from novice to those intimately familiar with array technology.
Protein Microarrays
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Author : Mark Schena
language : en
Publisher: Jones & Bartlett Learning
Release Date : 2005
Protein Microarrays written by Mark Schena and has been published by Jones & Bartlett Learning this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Science categories.
Protein Microarrays is one of the first books in this exciting new technology and will be an essential reference for most biologists and biotechnology professionals.
Microarray Technology And Its Applications
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Author : Uwe R. Müller
language : en
Publisher: Springer Science & Business Media
Release Date : 2005
Microarray Technology And Its Applications written by Uwe R. Müller and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Medical categories.
The genomics revolution would not have been possible without the 'parallelisation' offered by microarray technology. This technological - and commercial - success has been since emulated by other applications areas, with a tremendous amplification of innovation. This book describes the fundamentals and latest developments in microarray technology, as well as its future directions. It presents detailed overviews of the different techniques of fabricating microarrays, of the chemistries and preparative steps involved, of the different types of microarrays, and of the instrumentation and optical issues involved.
A Beginner S Guide To Microarrays
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Author : Eric M. Blalock
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-06-27
A Beginner S Guide To Microarrays written by Eric M. Blalock and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-27 with Science categories.
A Beginner's Guide to Microarrays addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and detailed protocols are provided for the technical steps and procedures involved in many facets of microarray technology, including: -Cleaning and coating glass slides, -Designing oligonucleotide probes, -Constructing arrays for the detection and quantification of different bacterial species, -Preparing spotting solutions, -Troubleshooting spotting problems, -Setting up and running a core facility, -Normalizing background signal and controlling for systematic variance, -Designing experiments for maximum effect, -Analyzing data with statistical procedures, -Clustering data with machine-learning protocols.
Microarray Methods And Protocols
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Author : Robert S. Matson
language : en
Publisher: CRC Press
Release Date : 2009-01-20
Microarray Methods And Protocols written by Robert S. Matson and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-20 with Medical categories.
A Step-by-Step Guide to Present and Future Uses of Microarray TechnologyMicroarray technology continues to evolve, taking on a variety of forms. From the spotting of cDNA and the in situ synthesis of oligonucleotide arrays now come microarrays comprising proteins, carbohydrates, drugs, tissues, and cells. With contributions from microarray experts
Microarray Technology In Practice
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Author : Steve Russell
language : en
Publisher: Academic Press
Release Date : 2008-11-21
Microarray Technology In Practice written by Steve Russell and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-21 with Science categories.
Using chips composed of thousands of spots, each with the capability of holding DNA molecules corresponding to a given gene, DNA microarray technology has enabled researchers to measure simultaneously gene expression across the genome. As with other large-scale genomics approaches, microarray technologies are broadly applicable across disciplines of life and biomedical sciences, but remain daunting to many researchers. This guide is designed to demystify the technology and inform more biologists about this critically important experimental technique. - Cohesive overview of the technology and available platforms, followed by detailed discussion of experimental design and analysis of microarray experiments - Up-to-date description of normalization methods and current methods for sample amplification and labeling - Deep focus on oligonucleotide design, printing, labeling and hybridization, data acquisition, normalization, and meta-analysis - Additional uses of microarray technology such as ChIP (chromatin immunoprecipitation) with hybridization to DNA arrays, microarray-based comparative genomic hybridization (CGH), and cell and tissue arrays
Dna Microarrays Part A Array Platforms And Wet Bench Protocols
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Author :
language : en
Publisher: Elsevier
Release Date : 2011-08-19
Dna Microarrays Part A Array Platforms And Wet Bench Protocols written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-19 with Science categories.
Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results. Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations. Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician. - Provides an overview of platforms - Includes experimental design and wet bench protocols - Presents statistical and data analysis methods, array databases, data visualization and meta-analysis