Microbial Biotechnology A Laboratory Manual For Bacterial Systems
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Microbial Biotechnology A Laboratory Manual For Bacterial Systems
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Author : Surajit Das
language : en
Publisher: Springer
Release Date : 2014-11-24
Microbial Biotechnology A Laboratory Manual For Bacterial Systems written by Surajit Das and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-11-24 with Science categories.
Microorganisms play an important role in the maintenance of the ecosystem structure and function. Bacteria constitute the major part of the microorganisms and possess tremendous potential in many important applications from environmental clean up to the drug discovery. Much advancement has been taken place in the field of research on bacterial systems. This book summarizes the experimental setups required for applied microbiological studies. Important background information, representative results, step by step protocol in this book will be of great use to the students, early career researchers as well as the academicians. The book describes many experiments covering the basic microbiological experiments to the applications of microbial systems for advanced research. Researchers in any field who utilize bacterial systems will find this book very useful. In addition to microbiology and bacteriology, this book will also find useful in molecular biology, genetics, and pathology and the volume should prove to be a valuable laboratory resource in clinical and environmental microbiology, microbial genetics and agricultural research. Unique features • Easy to follow by the users as the experiments have been written in simple language and step-wise manner. • Role of each reagents to be used in each experiment have been described which will help the beginners to understand quickly and design their own experiment. • Each experiment has been equipped with the coloured illustrations for proper understanding of the concept. • Trouble-shootings at the end of each experiment will be helpful in overcoming the problems faced by the users. • Flow-chart of each experiment will quickly guide the users in performing the experiments.
Advanced Methods In Molecular Biology And Biotechnology
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Author : Khalid Z. Masoodi
language : en
Publisher: Academic Press
Release Date : 2020-10-28
Advanced Methods In Molecular Biology And Biotechnology written by Khalid Z. Masoodi and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-28 with Science categories.
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Environmental Microbiology
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Author : Ian Pepper
language : en
Publisher: Academic Press
Release Date : 2011-10-13
Environmental Microbiology written by Ian Pepper and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-13 with Science categories.
For microbiology and environmental microbiology courses, this leading textbook builds on the academic success of the previous edition by including a comprehensive and up-to-date discussion of environmental microbiology as a discipline that has grown in scope and interest in recent years. From environmental science and microbial ecology to topics in molecular genetics, this edition relates environmental microbiology to the work of a variety of life science, ecology, and environmental science investigators. The authors and editors have taken the care to highlight links between environmental microbiology and topics important to our changing world such as bioterrorism and national security with sections on practical issues such as bioremediation, waterborne pathogens, microbial risk assessment, and environmental biotechnology.WHY ADOPT THIS EDITION? New chapters on: - Urban Environmental Microbiology - Bacterial Communities in Natural Ecosystems - Global Change and Microbial Infectious Disease - Microorganisms and Bioterrorism - Extreme Environments (emphasizing the ecology of these environments) - Aquatic Environments (now devoted to its own chapter- was combined with Extreme Environments) Updates to Methodologies: - Nucleic Acid -Based Methods: microarrays, phyloarrays, real-time PCR, metagomics, and comparative genomics - Physiological Methods: stable isotope fingerprinting and functional genomics and proteomics-based approaches - Microscopic Techniques: FISH (fluorescent in situ hybridization) and atomic force microscopy - Cultural Methods: new approaches to enhanced cultivation of environmental bacteria - Environmental Sample Collection and Processing: added section on air sampling
Microbiology And Biotechnology
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Author : P.T. Kalaichelvan
language : en
Publisher: MJP Publisher
Release Date : 2019-06-11
Microbiology And Biotechnology written by P.T. Kalaichelvan and has been published by MJP Publisher this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-06-11 with Medical categories.
Safety Guidelines Microbial Cell Counting Microscopic Observation of Microorganisms Appendix-I Appendix-II
Microbial Biotechnology
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Author : Ratul Saikia
language : en
Publisher: New India Publishing
Release Date : 2008-01-05
Microbial Biotechnology written by Ratul Saikia and has been published by New India Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-01-05 with Science categories.
Microbial Biotechnology is wide-ranging, multi-disciplinary activities which include recombinant DNA techniques, cloning and the application of microbes to the production of goods from bread to antibiotics. This book is an attempt to highlight the significant aspects of the vast subject area of microbial biotechnology likes bioinformatics tool for PCR primer designing, fungal biotransformations, bioremediation by microbes, natural products from fungi, microbial diversity etc to provide a complete overview of the subject. It also addresses the role of bacterial plasmid in xenobiotic degradation, antimicrobial resistance in bacteria, ultraviolet-B radiation effect on microbes and human health. The book will be valuable to the researchers, biologist, microbiologist, scientists, post graduate students of microbiology, agriculture, biotechnology and medical science also.
Applied Microbiology And Molecular Biology In Oilfield Systems
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Author : Corinne Whitby
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-10-13
Applied Microbiology And Molecular Biology In Oilfield Systems written by Corinne Whitby and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-10-13 with Science categories.
Applied Microbiology and Molecular Biology in Oil Field Systems addresses the major problems microbes cause in oil fields, (e.g. biocorrosion and souring) and how beneficial microbial activities may be exploited (e.g. MEOR and biofuels). The book describes theoretical and practical approaches to specific Molecular Microbiological Methods (MMM), and is written by leading authorities in the field from both academia and industry. The book describes how MMM can be applied to faciliate better management of oil reservoirs and downstream processes. The book is innovative in that it utilises real industrial case studies which gives useful technical and scientific information to researchers, engineers and microbiologists working with oil, gas and petroleum systems.
Experiments In Microbiology Plant Pathology And Biotechnology
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Author : K. R. Aneja
language : en
Publisher: New Age International
Release Date : 2007
Experiments In Microbiology Plant Pathology And Biotechnology written by K. R. Aneja and has been published by New Age International this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Microbiology categories.
Microorganisms Are Living Things Like Plants And Animals But Because Of Their Minute Size And Omnipresence, Performing Experiments With Microbes Requires Special Techniques And Equipment Apart From Good Theoretical Knowledge About Them. This Easy To Use Revised And Updated Edition Provides Knowledge About All The Three I.E., Techniques, Equipment And Principles Involved.The Notable Feature Of This Edition Is The Addition Of New Sections On Bacterial Taxonomy That Deals With The Criteria Used In Identification, Phylogeny And Current System Of Classification Of Procaryotes Based On The Second Edition Of Bergey Manual Of Systematic Bacteriology And The Section One On History Of Discovery Of Events That Covers Chronologically Important Events In Microbiology With The Contribution Of Pioneer Microbiologists Who Laid The Foundation Of The Science Of Microbiology. In The Subsequent Twenty-Two Sections, Various Microbiological Techniques Have Been Described Followed By Several Experiments Illustrating The Properties Of Microorganisms And Highlighting Their Involvement In Practically Every Sphere Of Life.Along With The Cultivation/Isolation/Purification Of Microbes, This Edition Also Contains Exercises Concerning Air, Soil, Water, Food, Dairy And AgriculturalMicrobiology, Bacterial Genetics, Plant Pathology, Plant Tissue Culture And Mushroom Production Technology. This Manual Contains 163 Experiments Spread Over 22 Different Sections. The Exercises Are Presented In A Simple Language With Explanatory Diagrams And A Brief Recapitulation Of Their Theory And Principle.The Exercises Are Selected By Keeping In Mind The Easy Availability Of Cultures, Culture Media And Equipment. Appendices At The End Of The Manual Provide A Reference To The Source For Obtaining Cultures Of Microbes, Culture Media And Preparation Of Various Stains, Reagents And Media In The Laboratory And Classification Of Procaryotes According To The First And Second Editions Of Bergey Is Manual Of Systematic Bacteriology.This Book Would Be Useful For The Undergraduate And Postgraduate Students, Teachers And Scientists In Diverse Areas Including The Biological Sciences, The Allied Health Services, Environmental Science, Biotechnology, Agriculture, Nutrition, Pharmacy And Various Other Professional Programmes Like Milk Processing Units, Diagnostic (Clinical) Microbiological Laboratories And Mushroom Cultivation At Small Or Large Scales.
Biotechnology Lab Techniques Culture Media Microscopy And Microbial Analysis
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Author :
language : en
Publisher: Deep Science Publishing
Release Date : 2025-04-13
Biotechnology Lab Techniques Culture Media Microscopy And Microbial Analysis written by and has been published by Deep Science Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-04-13 with Medical categories.
Welcome to the "Practical Handbook of Life Sciences". This comprehensive manual is designed to be an essential companion for students, researchers, and professionals in the field of life sciences. Whether you are just starting your journey into laboratory practices or looking to deepen your understanding of advanced techniques, this handbook provides clear and practical guidance. The world of life sciences is built upon a foundation of rigorous laboratory work, where precision and technique are paramount. This handbook begins with an introduction to basic laboratory practices, ensuring that readers develop a strong grasp of fundamental skills. From handling laboratory equipment to mastering techniques like smear preparation and staining of microorganisms, each chapter is structured to build upon the last, offering a progressive learning experience. Central to this handbook are detailed sections on laboratory equipment and tools, essential for conducting experiments effectively. Whether you are operating a compound microscope, utilizing an autoclave for sterilization, or conducting experiments with UV-Vis spectrophotometers, this handbook provides comprehensive insights into their functions and applications. Preparing media for cultivating microorganisms is a crucial skill covered extensively in this handbook. From nutrient broths to specialized agar types like McConkey and Chocolate agar, each recipe is meticulously detailed to ensure successful growth and isolation of pure microbial colonies. Techniques such as spread plating and streak plating are explained step-by-step, empowering researchers to isolate and study microbes with precision. Beyond basic techniques, this handbook delves into advanced topics such as the impact of environmental factors like UV radiation and pH on microbial growth. Techniques for assessing cell viability and methods for evaluating antibacterial efficacy of natural products are also explored in detail, reflecting the handbook's commitment to practical relevance in contemporary research. Additionally, this handbook encompasses techniques in molecular biology and biochemistry, from isolating nucleic acids and proteins to conducting gel electrophoresis and protein estimation assays. These techniques are pivotal for advancing research in genetics, biotechnology, and pharmaceutical sciences. Furthermore, the handbook extends its scope to include botanical and environmental sciences, featuring methods for estimating chlorophyll content, investigating organogenesis in plants, and assessing biochemical oxygen demand in water samples. Each chapter is authored by experts in their respective fields, ensuring that the content is not only informative but also reliable and up-to-date with current scientific practices. In conclusion, "Practical Handbook of Life Sciences" is more than just a reference guide; it is a practical companion that equips readers with the knowledge and skills necessary to excel in their scientific endeavors. Whether used in educational settings or research laboratories, this handbook serves as an indispensable tool for navigating the complexities of life sciences.
Molecular Biology Techniques
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Author : Heather B. Miller
language : en
Publisher: Academic Press
Release Date : 2011-10-18
Molecular Biology Techniques written by Heather B. Miller and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-18 with Science categories.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Petroleum Microbial Biotechnology Challenges And Prospects
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Author : Wael A. Ismail
language : en
Publisher: Frontiers Media SA
Release Date : 2017-09-08
Petroleum Microbial Biotechnology Challenges And Prospects written by Wael A. Ismail and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-09-08 with categories.
Petroleum hydrocarbons are both a product of, and rich substrate for, microorganisms from across all Domains of life. Rooted deeply in the history of microbiology, hydrocarbons have been studied as sources of carbon and energy for microorganisms for over a century. As global demand for petroleum and its refined products continues to rise, so do challenges associated with environmental pollution, oil well souring, infrastructure corrosion, oil recovery, transport, refining, and upgrading of heavy crude oils and bitumens. Advances in genomics, synthetic biology and metabolic engineering has invigorated interest in petroleum microbial biotechnology as interest grows in technologies for in situ methane production, biodesulfurization and biodenitrogenation, bio-upgrading of heavy crudes, microbial enhanced oil recovery, corrosion control, and biocatalysts for generating value-added products. Given the complexity of the global petroleum industry and the harsh conditions in which it operates, a deeper understanding of the ecophysiology of aerobic and anaerobic microbial communities that have associations with petroleum hydrocarbons is needed if robust technologies are to be deployed successfully. This research topic highlights recent advances in microbial enhanced oil recovery, methanogenic hydrocarbon metabolism and carbon dioxide sequestration, bioremediation, microbiologically influenced corrosion, biodesulfurization, and the application of metagenomics to better understand microbial communities associated with petroleum hydrocarbons.