Microtechnology For Cell Manipulation And Sorting

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Microtechnology For Cell Manipulation And Sorting

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Author : Wonhee Lee
language : en
Publisher: Springer
Release Date : 2016-10-05

Microtechnology For Cell Manipulation And Sorting written by Wonhee Lee and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-10-05 with Technology & Engineering categories.

This book delves into the recent developments in the microscale and microfluidic technologies that allow manipulation at the single and cell aggregate level. Expert authors review the dominant mechanisms that manipulate and sort biological structures, making this a state-of-the-art overview of conventional cell sorting techniques, the principles of microfluidics, and of microfluidic devices. All chapters highlight the benefits and drawbacks of each technique they discuss, which include magnetic, electrical, optical, acoustic, gravity/sedimentation, inertial, deformability, and aqueous two-phase systems as the dominant mechanisms utilized by microfluidic devices to handle biological samples. Each chapter explains the physics of the mechanism at work, and reviews common geometries and devices to help readers decide the type of style of device required for various applications. This book is appropriate for graduate-level biomedical engineering and analytical chemistry students, as well as engineers and scientists working in the biotechnology industry.

Microfluidics For Single Cell Analysis

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Author : Jin-Ming Lin
language : en
Publisher: Springer Nature
Release Date : 2019-08-28

Microfluidics For Single Cell Analysis written by Jin-Ming Lin and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-28 with Science categories.

This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.

Medical And Industrial Applications Of Microfluidic Based Cell Tissue Culture And Organs On A Chip Advances In Organs On A Chip And Organoids Technologies

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Author : Qasem Ramadan
language : en
Publisher: Frontiers Media SA
Release Date : 2019-10-16

Medical And Industrial Applications Of Microfluidic Based Cell Tissue Culture And Organs On A Chip Advances In Organs On A Chip And Organoids Technologies written by Qasem Ramadan and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-10-16 with categories.

Recent developments in microfluidics have demonstrated enormous potential of microscale cell culture for biology studies and recognized as instrumental in performing rapid and efficient experiments on small-sample volumes. Microfluidic-based cell culture is an area of research that keeps growing and gaining importance as a prominent technology, able to link scientific disciplines with industrial and clinical applications. In particular, organotypic cell culture and its integration in microfluidic devices would enable the realization of “in vivo-like” cell microenvironment within systems that are more amenable to automation and integration. Such remarkable advancement forms the foundation and motivation to transfer research from the laboratory to the field. Although the microfluidics and cell culture technologies have influenced many areas of science, significant research efforts are currently focus on finding methods to transform drug screening and toxicity testing from a system reliant on high-dose animal studies to one based primarily on human-relevant in vitro models. In line with regulatory developments precluding the use of animal testing, as well as fundamental differences in animal versus human, human in vitro methodologies are required to replace the animal-based testes while permitting physiologically relevant model equivalents for superior prediction. Organs-on-a-chip is an ambitious and rapidly growing technology that promise to bridge the gap between in vivo and in vitro studies and open wide possibilities in medical and industrial applications. However, many challenges are still ahead. This eBook present recent state-of-the-art works and critical reviews in organs-on-a-chip technology which highlight the new advances in this growing field with an emphasis on the interface between technological advancements and high impact applications.

Microfluidic Cell Culture Systems

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Author : Jeffrey T Borenstein
language : en
Publisher: Elsevier
Release Date : 2018-09-12

Microfluidic Cell Culture Systems written by Jeffrey T Borenstein and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-09-12 with Science categories.

Techniques for microfabricating intricate microfluidic structures that mimic the microenvironment of tissues and organs, combined with the development of biomaterials with carefully engineered surface properties, have enabled new paradigms in and cell culture-based models for human diseases. The dimensions of surface features and fluidic channels made accessible by these techniques are well-suited to the size scale of biological cells. Microfluidic Cell Culture Systems applies design and experimental techniques used in in microfluidics, and cell culture technologies to organ-on-chip systems. This book is intended to serve as a professional reference, providing a practical guide to design and fabrication of microfluidic systems and biomaterials for use in cell culture systems and human organ models. The book covers topics ranging from academic first principles of microfluidic design, to clinical translation strategies for cell culture protocols. The goal is to help professionals coming from an engineering background to adapt their expertise for use in cell culture and organ models applications, and likewise to help biologists to design and employ microfluidic technologies in their cell culture systems. This 2nd edition contains new material that strengthens the focus on in vitro models useful for drug discovery and development. One new chapter reviews liver organ models from an industry perspective, while others cover new technologies for scaling these models and for multi-organ systems. Other new chapters highlight the development of organ models and systems for specific applications in disease modeling and drug safety. Previous chapters have been revised to reflect the latest advances. Provides design and operation methodology for microfluidic and microfabricated materials and devices for organ-on-chip disease and safety models. This is a rapidly expanding field that will continue to grow along with advances in cell biology and microfluidics technologies. Comprehensively covers strategies and techniques ranging from academic first principles to industrial scale-up approaches. Readers will gain insight into cell-material interactions, microfluidic flow, and design principles. Offers three fundamental types of information: 1) design principles, 2) operation techniques, and 3) background information/perspectives. The book is carefully designed to strike a balance between these three areas, so it will be of use to a broad range of readers with different technical interests and educational levels.

Microfluidics For Cells And Other Organisms

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Author : Danny van Noort
language : en
Publisher: MDPI
Release Date : 2019-10-23

Microfluidics For Cells And Other Organisms written by Danny van Noort and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-10-23 with Technology & Engineering categories.

Microfluidics-based devices play an important role in creating realistic microenvironments in which cell cultures can thrive. They can, for example, be used to monitor drug toxicity and perform medical diagnostics, and be in a static-, perfusion- or droplet-based device. They can also be used to study cell-cell, cell-matrix or cell-surface interactions. Cells can be either single cells, 3D cell cultures or co-cultures. Other organisms could include bacteria, zebra fish embryo, C. elegans, to name a few.

Particles Separation In Microfluidic Devices

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Author : Takasi Nisisako
language : en
Publisher: MDPI
Release Date : 2020-12-10

Particles Separation In Microfluidic Devices written by Takasi Nisisako and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-12-10 with Technology & Engineering categories.

Microfluidic platforms are increasingly being used for separating a wide variety of particles based on their physical and chemical properties. In the past two decades, many practical applications have been found in chemical and biological sciences, including single cell analysis, clinical diagnostics, regenerative medicine, nanomaterials synthesis, environmental monitoring, etc. In this Special Issue, we invited contributions to report state-of-the art developments in the fields of micro- and nanofluidic separation, fractionation, sorting, and purification of all classes of particles, including, but not limited to, active devices using electric, magnetic, optical, and acoustic forces; passive devices using geometries and hydrodynamic effects at the micro/nanoscale; confined and open platforms; label-based and label-free technology; and separation of bioparticles (including blood cells), circulating tumor cells, live/dead cells, exosomes, DNA, and non-bioparticles, including polymeric or inorganic micro- and nanoparticles, droplets, bubbles, etc. Practical devices that demonstrate capabilities to solve real-world problems were of particular interest.

Nonmagnetic And Magnetic Quantum Dots

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Author : Vasilios N. Stavrou
language : en
Publisher: BoD – Books on Demand
Release Date : 2018-04-04

Nonmagnetic And Magnetic Quantum Dots written by Vasilios N. Stavrou and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-04 with Science categories.

The book entitled Nonmagnetic and Magnetic Quantum Dots is divided into two sections. In Section 1, the chapters are related to nonmagnetic quantum dots and their applications. More specifically, exact models and numerical methods have been presented to describe the analytical solution of the carrier wave functions, the quantum mechanical aspects of quantum dots, and the comparison of the latter to experimental data. Furthermore, methods to produce quantum dots, synthesis techniques of colloidal quantum dots, and applications on sensors and biology, among others, are included in this section. In Section 2, a few topics of magnetic quantum dots and their applications are presented. The section starts with a theoretical model to describe the magnetization dynamics in magnetic quantum dot array and the description of dilute magnetic semiconducting quantum dots and their applications. Additionally, a few applications of magnetic quantum dots in sensors, biology, and medicine are included in Section 2.

Pulsed Electric Fields Technology For The Food Industry

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Author : Javier Raso
language : en
Publisher: Springer Nature
Release Date : 2022-01-01

Pulsed Electric Fields Technology For The Food Industry written by Javier Raso and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-01 with Technology & Engineering categories.

Many novel technologies have been proposed in the attempt to improve existing food processing methods. Among emerging nonthermal technologies, high intensity pulsed electric fields (PEF) is appealing due to its short treatment times and reduced heating effects. This book presents information accumulated on PEF during the last 15 years by experienced microbiologists, biochemists, food technologists, and electrical and food engineers.

Processing Of Foods And Biomass Feedstocks By Pulsed Electric Energy

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Author : Eugene Vorobiev
language : en
Publisher: Springer Nature
Release Date : 2020-04-10

Processing Of Foods And Biomass Feedstocks By Pulsed Electric Energy written by Eugene Vorobiev and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-04-10 with Technology & Engineering categories.

This book presents a comprehensive range of research on pulsed electric energy used in food processing, including sections on the fundamentals of electroporation and important techniques for the estimation of electroporation effects in various foods and biomass feedstocks. By focusing on application over theory, this book presents researchers with practical steps for processing techniques such as solid-liquid extraction, pressing, osmotic dehydration, drying, freezing and cooking. Special interest is given to the selective recovery and extraction of sugar, inulin, starch, proteins, polysaccharides, polyphenols, pigments, flavor compounds, phytochemicals and other of high-value components from food biomasses such as fruits and vegetables, leaves, herbs, mushrooms, microalgae and suspensions of cells. Processing of Foods and Biomass Feedstocks by Pulsed Electric Energy presents a singular overview of the biorefinery applications of pulsed electric energy for the processing of wastes and non-food biomasses such as root and tuber crops, grape waste, lignocellulosic biomass, oil crops and residues and seeds and peels of exotic and citrus fruits. The book begins by presenting general information on the fundamentals of electroporation and information on the procedures and protocols involved. Further chapters focus on the specific food processing operations involved and biorefinery applications for the processing of wastes and non-food biomasses. All of the relevant and up-to-date information any researcher needs on pulsed electric energy in food processing is presented here in this text.

Handbook On Biological Warfare Preparedness

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Author : S.J.S. Flora
language : en
Publisher: Academic Press
Release Date : 2019-10-05

Handbook On Biological Warfare Preparedness written by S.J.S. Flora and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-10-05 with Medical categories.

Handbook on Biological Warfare Preparedness provides detailed information on biological warfare agents and their mode of transmission and spread. In addition, it explains methods of detection and medical countermeasures, including vaccine and post-exposure therapeutics, with specific sections detailing diseases, their transmission, clinical signs and symptoms, diagnosis, treatment, vaccines, prevention and management. This book is useful reading for researchers and advanced students in toxicology, but it will also prove helpful for medical students, civil administration, medical doctors, first responders and security forces. As the highly unpredictable nature of any event involving biological warfare agents has given rise to the need for the rapid development of accurate detection systems, this book is a timely resource on the topic. Introduces different bacterial and viral agents, including Ebola and other emerging threats and toxins Discusses medical countermeasures, including vaccines and post-exposure therapeutics Includes a comprehensive review of current methods of detection