Modern Optics Electronics And High Precision Techniques In Cell Biology
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Modern Optics Electronics And High Precision Techniques In Cell Biology
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Author : Gerhard Isenberg
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Modern Optics Electronics And High Precision Techniques In Cell Biology written by Gerhard Isenberg and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
In spite of tremendous scientific progress over the past years, cell biologists do not yet understand the fundamental processes that determine the life cy cle of a cell. Such are: cell movement and cell spreading, cell division, cell communication, cell signaling, cell regeneration and cell death. Biochemistry has enabled us to recognize and to isolate an overwhelming number of new proteins. In vitro assays and the reinjection of proteins into cells and tissues have provided insights into molecular functions and cellular mechanisms. The renaissance of the genetic approach by applying restriction enzymes and vectors, PCR and antisense technology has enabled us to overexpress certain cellular products, to make altered constructs of cell components or to create "knock-out" mutants that entirely lack the factor of interest. Amazingly en ough, all these molecular toys have led to a stream of information but not, in a comparable degree, to a better understanding. Has the puzzle become too complex to get solved; or are the windows too small that we are looking through? As an attempt to answer both questions, the aim of the present mono graph Modern Optics, Electronics and High Precision Techniques in Cell Biol ogy is first to provide cell and molecular biologists with a whole new scope of easily applicable techniques including brand-new optical, biophysical, physicochemical and biosensoric devices. Secondly, these newly developed techniques allow us to look at cells and biological systems as a whole.
Image Analysis
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Author : Donat P. Hader
language : en
Publisher: CRC Press
Release Date : 2000-08-23
Image Analysis written by Donat P. Hader and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-08-23 with Medical categories.
Automatic image analysis has become an important tool in many fields of biology, medicine, and other sciences. Since the first edition of Image Analysis: Methods and Applications, the development of both software and hardware technology has undergone quantum leaps. For example, specific mathematical filters have been developed for quality enhancement of original images and for extraction of specific features of interest. Also, more complex programs have been developed for the analysis of object forms in distinguishing cancer cells from normal tissue cells. Just as significant, three-dimensional analysis of proteins, organelles, or macroscopic objects is even more complex. In addition, recent space-based experiments have optimized techniques for the extraction of movement parameters of numerous motile objects. The second edition of Image Analysis: Methods and Applications addresses all these new developments. Moreover, two new chapters have been added. One focuses on images on the internet, and the other discusses microscope image restoration. These chapters add significantly to the existing body of information on Internet communication protocol and environment as well as to that on image file formats considerations. The materials also include a list of internet Web sites that pertain to digital images and software along with those that relate to image processing. With these considerations in mind, Image Analysis: Methods and Application, Second Edition is of incalculable value to professionals, academics, and users of all aspects of image analysis in biology and other areas of science.
Understanding Light Microscopy
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Author : Jeremy Sanderson
language : en
Publisher: John Wiley & Sons
Release Date : 2019-03-28
Understanding Light Microscopy written by Jeremy Sanderson and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-03-28 with Science categories.
Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.
Fluorescence Spectroscopy And Microscopy In Biology
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Author : Radek Šachl
language : en
Publisher: Springer Nature
Release Date : 2023-04-27
Fluorescence Spectroscopy And Microscopy In Biology written by Radek Šachl and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-04-27 with Science categories.
This book provides the reader with an updated comprehensive view of the rapidly developing and fascinating field of fluorescence spectroscopy and microscopy. In recent years, fluorescence spectroscopy and microscopy have experienced rapid technological development, which has enabled the detection and monitoring of single molecules with high spatial and temporal resolution. Thanks to these developments, fluorescence has become an even more popular method in physical, biological and related fields. This book guides the reader through both basic and advanced fluorescence spectroscopy and microscopy approaches with a focus on their applications in membrane and protein biophysics. Each of the four parts: A - Fluorescence Spectroscopy, B - Fluorescence Microscopy, C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes and D - Applications of Fluorescence Spectroscopy to protein studies are written by experts within the field. The book is intended for both complete beginners who want to quickly orient themselves in the large number of existing fluorescent methods, as well as for advanced readers who are interested in particular methods and their proper use.
Biomedical Imaging
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Author : Reiner Salzer
language : en
Publisher: John Wiley & Sons
Release Date : 2012-04-11
Biomedical Imaging written by Reiner Salzer and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-04-11 with Technology & Engineering categories.
This book presents and describes imaging technologies that can be used to study chemical processes and structural interactions in dynamic systems, principally in biomedical systems. The imaging technologies, largely biomedical imaging technologies such as MRT, Fluorescence mapping, raman mapping, nanoESCA, and CARS microscopy, have been selected according to their application range and to the chemical information content of their data. These technologies allow for the analysis and evaluation of delicate biological samples, which must not be disturbed during the profess. Ultimately, this may mean fewer animal lab tests and clinical trials.
Fundamentals Of Optical Engineering
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Author : S. Singh
language : en
Publisher: Discovery Publishing House
Release Date : 2009
Fundamentals Of Optical Engineering written by S. Singh and has been published by Discovery Publishing House this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with Lenses categories.
Optical Imaging Techniques In Cell Biology Second Edition
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Author : Guy Cox
language : en
Publisher: CRC Press
Release Date : 2012-06-04
Optical Imaging Techniques In Cell Biology Second Edition written by Guy Cox and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-06-04 with Medical categories.
Optical Imaging Techniques in Cell Biology, Second Edition covers the field of biological microscopy, from the optics of the microscope to the latest advances in imaging below the traditional resolution limit. It includes the techniques—such as labeling by immunofluorescence and fluorescent proteins—which have revolutionized cell biology. Quantitative techniques such as lifetime imaging, ratiometric measurement, and photoconversion are all covered in detail. Expanded with a new chapter and 40 new figures, the second edition has been updated to cover the latest developments in optical imaging techniques. Explanations throughout are accurate, detailed, but as far as possible non-mathematical. This edition includes appendices with useful practical protocols, references, and suggestions for further reading. Color figures are integrated throughout.
Advances In Planar Lipid Bilayers And Liposomes
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Author : A. Leitmannova Liu
language : en
Publisher: Academic Press
Release Date : 2011-08-09
Advances In Planar Lipid Bilayers And Liposomes written by A. Leitmannova Liu and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-09 with Science categories.
Advances in Planar Lipid Bilayers and Liposomes, Volume 6, continues to include invited chapters on a broad range of topics, covering both main arrangements of the reconstituted system, namely planar lipid bilayers and spherical liposomes. The invited authors present the latest results in this exciting multidisciplinary field of their own research group. Many of the contributors working in both fields over many decades were in close collaboration with the late Prof. H. Ti Tien, the founding editor of this book series. There are also chapters written by some of the younger generation of scientists included in this series. This volume keeps in mind the broader goal with both systems, planar lipid bilayers and spherical liposomes, which is the further development of this interdisciplinary field worldwide. Contributions from newcomers and established and experienced researchers Exploring theoretically and experimentally the planar lipid bilayer systems and spherical liposomes This volume is dedicated to mark the Bilayer Lipid Membranes 45th anniversary
Optical Imaging Techniques In Cell Biology
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Author : Guy Cox
language : en
Publisher: CRC Press
Release Date : 2006-11-06
Optical Imaging Techniques In Cell Biology written by Guy Cox and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-11-06 with Science categories.
Since the word microscopy was coined in 1656, the evolution of the instrument has had a long and convoluted history. Plagued with problems of chromatic aberration, spherical aberration, and challenges with illumination and resolution, the microscope's technical progression happened in a series of fits and starts until the late 19th century. After E
New Techniques Of Optical Microscopy And Microspectroscopy
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Author : Cherry
language : en
Publisher: CRC Press
Release Date : 1991
New Techniques Of Optical Microscopy And Microspectroscopy written by Cherry and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with Science categories.
The last few years have seen an upsurge of interest in the study of cells by optical microscopy. The advent of new techniques such as confocal microscopy and the availability of extremely sensitive digital imaging devices are revolutioniz-ing the field. A number of groups have developed new ways of making spectroscopic measurements at the microscopic level, accompanied by the introduction of appropriate sen-sor molecules for cellular assays. The aim of this volume will be to bring together the various advances in order to provide the reader with an up-to-date account of what can now be achieved with modern optical microscopic methods.