Molecular Diagnostic Methods For Bacteria And Fungi Detection
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Molecular Diagnostic Methods For Bacteria And Fungi Detection
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Author : Laura Cerqueira
language : en
Publisher: Frontiers Media SA
Release Date : 2024-06-12
Molecular Diagnostic Methods For Bacteria And Fungi Detection written by Laura Cerqueira and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-06-12 with Science categories.
Microorganisms, like bacteria and fungi, are ubiquitous worldwide and can have different roles in human’s lives. Some will bring beneficial effects which are exploited and used in industrial and agricultural sectors. Contrariwise, some are responsible for several life-threatening diseases. Microbial analysis, surveillance and research is therefore crucial. Until recently, the classical culturing methods were widely used to study bacteria and fungi. However these methods, although considered the gold standard, are becoming now obsolete since they tend to be time-consuming, have low sensitivity and are unable to detect some cellular morphological states, as the viable but non-culturable (VBNC) state, leading to false negative results. Moving away from the classical methods, microbial detection is now evolving to new effective and rapid diagnostics.
Molecular Microbiology
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Author : David H. Persing
language : en
Publisher: John Wiley & Sons
Release Date : 2016-04-01
Molecular Microbiology written by David H. Persing and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-01 with Science categories.
Presenting the latest molecular diagnostic techniques in one comprehensive volume The molecular diagnostics landscape has changed dramatically since the last edition of Molecular Microbiology: Diagnostic Principles and Practice in 2011. With the spread of molecular testing and the development of new technologies and their opportunities, laboratory professionals and physicians more than ever need a resource to help them navigate this rapidly evolving field. Editors David Persing and Fred Tenover have brought together a team of experienced researchers and diagnosticians to update this third edition comprehensively, to present the latest developments in molecular diagnostics in the support of clinical care and of basic and clinical research, including next-generation sequencing and whole-genome analysis. These updates are provided in an easy-to-read format and supported by a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors. Molecular Microbiology: Diagnostic Principles and Practice Presents the latest basic scientific theory underlying molecular diagnostics Offers tested and proven applications of molecular diagnostics for the diagnosis of infectious diseases, including point-of-care testing Illustrates and summarizes key concepts and techniques with detailed figures and tables Discusses emerging technologies, including the use of molecular typing methods for real-time tracking of infectious outbreaks and antibiotic resistance Advises on the latest quality control and quality assurance measures Explores the increasing opportunities and capabilities of information technology Molecular Microbiology: Diagnostic Principles and Practice is a textbook for molecular diagnostics courses that can also be used by anyone involved with diagnostic test selection and interpretation. It is also a useful reference for laboratories and as a continuing education resource for physicians. If you are looking for online access to the latest clinical microbiology content, please visit www.wiley.com/learn/clinmicronow.
Molecular Methods In Plant Disease Diagnostics
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Author : Neil Boonham
language : en
Publisher: CABI
Release Date : 2016-04-19
Molecular Methods In Plant Disease Diagnostics written by Neil Boonham and has been published by CABI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-19 with Science categories.
Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.
Advanced Techniques In Diagnostic Microbiology
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Author : Yi-Wei Tang
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-09-14
Advanced Techniques In Diagnostic Microbiology written by Yi-Wei Tang and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-09-14 with Medical categories.
In the United States, hospitals annually report over 5 million cases of infectious-disease-related illnesses: clinical microbiology laboratories in these hospitals are engaged in detecting and identifying the pathogenic microorganisms in clinical specimens collected from these patients with suspected infections. Clearly, the timely and accurate detection/identification of these microbial pathogens is critical for patient treatment decisions and outcomes for millions of patients each year. Despite an appreciation that the outcome of an infectious-disease-related illness is directly related to the time required to detect and identify a microbial pathogen, clinical microbiology laboratories in the United States as well as worldwide have long been hampered by traditional culture-based assays, which may require prolonged incubation time for slowly growing microorganisms such as Mycobacterium tuberculosis. Moreover, traditional culture-based assays often require multiple steps with additional time needed for discernment of species and/or detection of antimicrobial resistance. Finally, these traditional, slow multistep culture-based assays are labor-intensive and required skilled clinical microbiologists at the bench. Over the past several decades, advanced molecular techniques in diagnostic microbiology quietly have been revolutionizing the practice of clinical microbiology in the hospital setting. Indeed, molecular diagnostic testing in general and nucleic-acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. There is no question that the development of rapid molecular techniques for nucleic acid amplification/characterization combined with automation and user-friendly software has greatly broadened the diagnostic capabilities of the clinical microbiology laboratory. These technical advances in molecular microbiology over the first decade of the 21st Century have profoundly influenced the physical structure of clinical microbiology laboratories as well as their staffing patterns, workflow, and turnaround time. These molecular microbiology advances have also resulted in the need for a revised and updated second edition of Advanced Techniques in Diagnostic Microbiology. This second edition again provides an updated and comprehensive description of the ongoing evolution of molecular methods for the diagnosis of infectious diseases. In addition, many new chapters have been added, including a chapter on the clinical interpretation and relevance of advanced technique results. The second edition, like the first edition, includes both a “techniques” section describing the latest molecular techniques and an “applications” section describing how these advanced molecular techniques are being used in the clinical setting. Finally, the second edition, like the first edition, utilizes a diverse team of authors who have compiled chapters that provide the reader with comprehensive and useable information on advanced molecular microbiology techniques.
Common Eye Infections
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Author : Imtiaz Chaudhry
language : en
Publisher: BoD – Books on Demand
Release Date : 2013-05-08
Common Eye Infections written by Imtiaz Chaudhry and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-05-08 with Medical categories.
Since ocular infections are one of the most frequent occurrences in ophthalmology, the treatment for these infections must be fast, precise and effective. In order to address this goal, it is important to identify and characterize the culprit microorganisms involved in the pathogenesis of ocular infections. Clinical diagnosis of ocular infections can be confirmed by several techniques based on microbiological test of ocular samples. Some of these techniques include classic microbiological testing in which it is necessary to isolate microorganisms to characterize them by biochemical analysis which require significant resources and timing.
Pcr For Clinical Microbiology
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Author : Ian W.J. Carter
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-07-03
Pcr For Clinical Microbiology written by Ian W.J. Carter and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-07-03 with Science categories.
Not another textbook, but a valuable tool for doctors and microbiologists wanting to know how to set up a PCR diagnostic microbiology laboratory according to current regulatory standards and perform assays supplied with patient clinical diagnostic criteria and easy to follow protocols. Whether laboratories are using commercial kits or in-house methods developed in their own laboratories or adopted from published methods, all clinical microbiology laboratories need to be able to understand, critically evaluate, perform and interpret these tests according to rigorous and clinically appropriate standards and international guidelines. The cost and effort of development and evaluation of in-house tests is considerable and many laboratories do not have the resources to do so. This compendium is a vehicle to improve and maintain the clinical relevance and high quality of diagnostic PCR. It is a unique collection of; guidelines for PCR laboratory set up and quality control, test selection criteria, methods and detailed step by step protocols for a diagnostic assays in the field of molecular microbiology. The structure of the book provides the PCR fundamentals and describes the clinical aspects and diagnosis of infectious disease. This is followed by protocols divided into; bacteria, virus, fungi and parasites, and susceptibility screens. The inclusion of medical criteria and interpretation adds value to the compendium and benefits clinicians, scientists, researchers and students of clinical diagnostic microbiology
Diagnostic Approaches For Aspergillus Infections
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Author : Juergen Prattes
language : en
Publisher: Frontiers Media SA
Release Date : 2019-05-16
Diagnostic Approaches For Aspergillus Infections written by Juergen Prattes and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-16 with categories.
Infections caused by Aspergillus spp. are associated with devastating mortality rates. Early and reliable diagnosis and subsequent rapid initiation of appropriate antifungal therapy has shown to improve survival significantly, at least for invasive Aspergillosis. Early diagnosis of Aspergillus associated infections, therefore, represents a cornerstone in successful management of these diseases. Current state of the art diagnostic approaches, new insights in epidemiology and established biomarkers as well as an outlook for future diagnostic options in the armamentarium of diagnostics tools are highlighted in this eBook.
Diagnosis Of Fungal Infections
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Author : Johan Maertens
language : en
Publisher: CRC Press
Release Date : 2007-08-29
Diagnosis Of Fungal Infections written by Johan Maertens and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-08-29 with Medical categories.
Analyzing a key cause of infectious morbidity in immunosuppressed and immunocompromised patients, this source spans the most recent strategies to improve and expedite the diagnosis, identification, and treatment of fungal infections. With authoritative contributions from experienced clinicians in the field, this reference tracks developments in ima
Mandell Douglas And Bennett S Principles And Practice Of Infectious Diseases
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Author : John E. Bennett, MD, MACP
language : en
Publisher: Elsevier Health Sciences
Release Date : 2014-08-28
Mandell Douglas And Bennett S Principles And Practice Of Infectious Diseases written by John E. Bennett, MD, MACP and has been published by Elsevier Health Sciences this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-08-28 with Medical categories.
After thirty five years, Mandell, Douglas, and Bennett’s Principles and Practice of Infectious Diseases, 8th Edition is still the reference of choice for comprehensive, global guidance on diagnosing and treating the most challenging infectious diseases. Drs. John E. Bennett and Raphael Dolin along with new editorial team member Dr. Martin Blaser have meticulously updated this latest edition to save you time and to ensure you have the latest clinical and scientific knowledge at your fingertips. With new chapters, expanded and updated coverage, increased worldwide perspectives, and many new contributors, Mandell, Douglas, and Bennett’s Principles and Practice of Infectious Diseases, 8th Edition helps you identify and treat whatever infectious disease you see. Get the answers to questions you have with more in-depth coverage of epidemiology, etiology, pathology, microbiology, immunology, and treatment of infectious agents than you'll find in any other infectious disease resource. Find the latest diagnoses and treatments for currently recognized and newly emerging infectious diseases, such as those caused by avian and swine influenza viruses. Put the latest knowledge to work in your practice with new or completely revised chapters on influenza (new pandemic strains); new Middle East respiratory syndrome (MERS) virus; probiotics; antibiotics for resistant bacteria; antifungal drugs; new antivirals for hepatitis B and C; Clostridium difficile treatment; sepsis; advances in HIV prevention and treatment; viral gastroenteritis; Lyme disease; Helicobacter pylori; malaria; infections in immunocompromised hosts; immunization (new vaccines and new recommendations); and microbiome. Benefit from fresh perspectives and global insights from an expanded team of international contributors. Find and grasp the information you need easily and rapidly with newly added chapter summaries. These bulleted templates include diagnosis, therapy, and prevention and are designed as a quick summary of the chapter and to enhance relevancy in search and retrieval on Expert Consult. Stay current on Expert Consult with a thorough and regularly scheduled update program that ensures access to new developments in the field, advances in therapy, and timely information. Access the information you need easily and rapidly with new succinct chapter summaries that include diagnosis, therapy, and prevention. Experience clinical scenarios with vivid clarity through a richly illustrated, full-color format that includes 1500 photographs for enhanced visual guidance.
Ecologically Based Integrated Pest Management
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Author : Opender Koul
language : en
Publisher: CABI
Release Date : 2007
Ecologically Based Integrated Pest Management written by Opender Koul and has been published by CABI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Technology & Engineering categories.
This book, intended for all those involved in studying entomology, crop protection and pest management, has 18 review chapters on topics ranging from the ecological effects of chemical control practices to the ecology of predator-prey and parasitoid-host systems.