Monoclonal Antibodies And Functional Cell Lines
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Monoclonal Antibodies And Functional Cell Lines
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Author : Roger H. Kennett
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-08
Monoclonal Antibodies And Functional Cell Lines written by Roger H. Kennett and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-08 with Medical categories.
This volume serves as a follow-up to our previous book, MonoclonalAntibodies Hybridomas: A New Dimension in Biological Analyses. We continue the theme of monoclonal antibodies and their applications, attempting to cover some of the areas not covered in the previous volume. We again include an appendix de scribing methods useful to those who ar-e beginning to apply these techniques in their own laboratories. This volume will be followed by another concentrating on the combination of monoclonal antibody techniques with molecular genetic techniques to study structure/function relationships at the level of both the gene and gene product. Roger H. Kennett Kathleen B. Bechtol Philadelphia, Pennsylvania Thomas J. McKearn Princeton, New Jersey IX Acknowledgments Roger Kennett acknowledges the patience and support of his wife, Carol, and his family, friends, and colleagues during the work on this volume, and again thanks, above all, the Lord, Jesus Christ. Kathleen Bechtol wishes to thank colleagues and friends for their support and understanding during the months of preparation of this volume. Tom McKearn acknowledges and thanks his wife, Pat, and his family for their support and encouragement. Xl Contents PART I INTRODUCTION 1 Introduction: Reflections on Nine Years of Monoclonal Antibodies from Hybridomas 3 ROGER H. KENNETT, KATHLEEN B. BECHTOL, AND THOMAS J. McKEARN 1. Biotechnology'S "Coming of Age". . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 II. Monoclonal Antibodies-An Overview of Applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 III. Commercialization of Monoclonal Antibody Technology.. . . .. ... . .... .... .. . ... . . 10 References. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 . . . . . . . . . . . . . . . . . . . . .
Monoclonal Antibodies And Functional Cell Lines
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Author : Roger H Kennett
language : en
Publisher:
Release Date : 1984-06-01
Monoclonal Antibodies And Functional Cell Lines written by Roger H Kennett and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984-06-01 with categories.
Monoclonal Hybridoma Antibodies
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Author : John G.R. Hurrell
language : en
Publisher: CRC Press
Release Date : 2018-05-04
Monoclonal Hybridoma Antibodies written by John G.R. Hurrell and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-04 with Health & Fitness categories.
The first section of this volume is aimed to provide a comprehensive review of the many varied and often empirically derived techniques and procedures currently in use to produce monoclonal hybridoma cell lines and to characterize the antibodies secreted. The goal has been achieved with the chapter contributed by Zola and Brookes who, as each step in the process of hybridoma production and antibody characterisation is reviewed, have provided an experimental procedure found to be satisfactory in their laboratory.The second section of this volume is designed to provide a review of areas in which monoclonal hybridoma antibodies have been of particular advantage. This is a rapidly advancing field which could not be thoroughly reviewed in a single volume.
Monoclonal Antibodies In Biotechnology
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Author : Kenneth C. McCullough
language : en
Publisher: Cambridge University Press
Release Date : 1990-04-19
Monoclonal Antibodies In Biotechnology written by Kenneth C. McCullough and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-04-19 with Medical categories.
Monoclonal antibodies are one of the most exciting developments in biotechnology in recent years; this book provides a comprehensive description of principles, methodologies and applications of this powerful technology to modern science and industry.
Lymphocyte Hybridomas
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Author : F. Melchers
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Lymphocyte Hybridomas written by F. Melchers and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
Sponsored by the National Cancer Institute (NIH)
A Practical Guide To Monoclonal Antibodies
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Author : J. Eryl Liddell
language : en
Publisher: John Wiley & Sons
Release Date : 1991-08-26
A Practical Guide To Monoclonal Antibodies written by J. Eryl Liddell and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991-08-26 with Medical categories.
Includes all of the information required to produce monoclonal antibodies in the laboratory and to prepare them for use in a multitude of given applications. Production procedures are treated in chronological order, beginning with basic tissue culture techniques, immunization strategies and screening test design, followed by production of hybridoma cell lines and basic antibody characterization, purification and labeling. Each chapter contains explanatory text on each step with comparative analysis of methods where appropriate. All necessary experimental protocols are presented in a self-contained format that is easy to follow in the laboratory. Alternative protocols are provided where relevant; for others not included in full, source references are presented. Surveys the current status of human hybridoma production and antibody engineering using molecular biology techniques.
Monoclonal Antibodies
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Author : Mary A. Ritter
language : en
Publisher: Cambridge University Press
Release Date : 1995-01-26
Monoclonal Antibodies written by Mary A. Ritter and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-01-26 with Medical categories.
This book aims to provide a unique combination of the production (by both cellular and molecular biology techniques), structure and functional characteristics of monoclonal antibodies, together with detailed discussions of the various analytic, diagnostic and therapeutic applications.
Monoclonal Antibody Production
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Author : National Research Council
language : en
Publisher: National Academies Press
Release Date : 1999-05-06
Monoclonal Antibody Production written by National Research Council and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-05-06 with Medical categories.
The American Anti-Vivisection Society (AAVS) petitioned the National Institutes of Health (NIH) on April 23, 1997, to prohibit the use of animals in the production of mAb. On September 18, 1997, NIH declined to prohibit the use of mice in mAb production, stating that "the ascites method of mAb production is scientifically appropriate for some research projects and cannot be replaced." On March 26, 1998, AAVS submitted a second petition, stating that "NIH failed to provide valid scientific reasons for not supporting a proposed ban." The office of the NIH director asked the National Research Council to conduct a study of methods of producing mAb. In response to that request, the Research Council appointed the Committee on Methods of Producing Monoclonal Antibodies, to act on behalf of the Institute for Laboratory Animal Research of the Commission on Life Sciences, to conduct the study. The 11 expert members of the committee had extensive experience in biomedical research, laboratory animal medicine, animal welfare, pain research, and patient advocacy (Appendix B). The committee was asked to determine whether there was a scientific necessity for the mouse ascites method; if so, whether the method caused pain or distress; and, if so, what could be done to minimize the pain or distress. The committee was also asked to comment on available in vitro methods; to suggest what acceptable scientific rationale, if any, there was for using the mouse ascites method; and to identify regulatory requirements for the continued use of the mouse ascites method. The committee held an open data-gathering meeting during which its members summarized data bearing on those questions. A 1-day workshop (Appendix A) was attended by 34 participants, 14 of whom made formal presentations. A second meeting was held to finalize the report. The present report was written on the basis of information in the literature and information presented at the meeting and the workshop.
Monoclonal Antibodies T Cell Products
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Author : David H Katz
language : en
Publisher: CRC Press
Release Date : 2019-06-04
Monoclonal Antibodies T Cell Products written by David H Katz and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-06-04 with Medical categories.
First Published in 1982, this book offers a full, comprehensive guide into the applications of Monoclonal Antibodies. Carefully compiled and filled with a vast repertoire of notes, diagrams, and references this book serves as a useful reference for Students of Medicine, and other practitioners in their respective fields.
Human Hybridomas And Monoclonal Antibodies
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Author : Edgar Engleman
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Human Hybridomas And Monoclonal Antibodies written by Edgar Engleman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Medical categories.
Soon after Kohler and Milstein described the use of somatic cell hybridization for the production of murine monoclonal antibodies of desired specificity, this relatively simple technique became widely applied. Indeed, production of murine monoclonal antibodies is now considered routine by immunologists and nonimmunologists alike. However, as heterologous proteins, mouse monoclonal antibodies have one major limitation: they are immunogenic in man and, hence, their use in vivo is severely limited. An obvious solution to this problem is to produce human hybridomas with the same techniques used for the production of rodent hybrids. Unfortunately, the history of human hybridomas has been marked by substantive and often exasperating tech nical problems, and the first reports of hybrids secreting human immu noglobulin of desired specificity did not appear until 1980. These reports were met with initial enthusiasm, but it soon became apparent that while human lymphocytes might be fused, their frequency, level of Ig synthesis, and stability were such that production of human antibodies with this method was neither routine nor practical. Nonetheless, a sufficient number of investiga tors persevered, and during the next 5 years relatively efficient B-cell fusion partners as well as improved methods of Epstein-Barr virus transformation were developed. Generation of human T -T hybrids has also been achieved, although problems of chromosomal stability remain a substantial obstacle, more so than with B-cell lines.