Mycobacteria Protocols
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Mycobacteria Protocols
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Author : Tanya Parish
language : en
Publisher: Humana Press
Release Date : 2011-05-26
Mycobacteria Protocols written by Tanya Parish and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-05-26 with Science categories.
Due to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need. Mycobacteria Protocols, Second Edition updates and refines the methods of the well-received first edition while adding newly developed methods culled from the most cutting-edge research in the field. Ranging from the basics of sub-cellular fractionation to advanced methods using specialized growth conditions and whole genome, transcriptome and proteome analysis, the chapters conform to the Methods in Molecular BiologyTM series format, providing step-by-step laboratory protocols, lists of necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Mycobacteria Protocols, Second Edition is the perfect resource to promote and stimulate further research into these intriguing, important and most fractious of bacteria.
Mycobacterium Tuberculosis Protocols
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Author : Tanya Parish
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-01
Mycobacterium Tuberculosis Protocols written by Tanya Parish and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-01 with Medical categories.
The aim of this book is to provide detailed protocols for studying the molecular biology of the pathogen Mycobacterium tuberculosis, and its int- actions with host cells. As established mycobacterial laboratories move - wards exploiting the genome, and laboratories with expertise in other fields apply them to mycobacteria, both traditional and novel methodologies need to be reviewed. Thus the chapters in Mycobacterium tuberculosis Protocols range from perspectives on storage of strains and safety issues to the application of the latest functional genomics technologies. The last few years have been remarkable ones for research into M. tuber- losis. The most important landmark by far has been the completion of the genome sequence of the widely studied H37Rv strain (1). We can now predict every protein and RNA molecule made by the pathogen. This information is or will soon be enriched by the addition of genome sequences of other strains from the M. tuberculosis complex: a second strain of M. tuberculosis, My- bacterium bovis, and the vaccine strain, M. bovis BCG. Valuable comparative data will also be provided by the genome sequences of Mycobacterium leprae, Mycobacterium avium, and Streptomyces coelicolor. Another recent milestone for M. tuberculosis has been the development of efficient mutagenesis me- odologies, the lack of which has been a major handicap in functional studies.
Mycobacterium Ulcerans
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Author : Gerd Pluschke
language : en
Publisher: Humana
Release Date : 2022-10-14
Mycobacterium Ulcerans written by Gerd Pluschke and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-10-14 with Science categories.
This volume explores the latest techniques used to study Mycobacterium ulcerans, and more specifically M. ulcerans disease (Buruli ulcer). The chapters in this book are organized into three parts and cover methods for the detection of M. ulcerans and the analysis of host-pathogen interaction; the quantification and characterization of mycolactone, the macrolide toxin of M. ulcerans; and drug development against M. ulcerans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mycobacterium ulcerans: Methods and Protocols is a valuable resource that helps scientists advance their research on Buruli ulcer, which is still an under-researched field in infection biology.
Diagnostic Bacteriology Protocols
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Author : Louise O’Connor
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Diagnostic Bacteriology Protocols written by Louise O’Connor and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
The field of bacterial diagnostics has seen unprecedented advances in recent years. The increased need for accurate detection and identification of bacteria in human, animal, food, and environmental samples has fueled the development of new techniques. The field has seen extensive research aided by the information from bacterial genome sequencing projects. Although traditional methods of bacterial detection and identification remain in use in laboratories around the world, there is now a growing trend toward the use of nucleic ac- based diagnostics and alternative biochemically and immunologically based formats. The ultimate goal of all diagnostic tests is the accurate detection, identification, or typing of microorganisms in samples of interest. Although the resulting information is of obvious use in the areas of patient management, animal health, and quality control, it is also of use in monitoring routes of infection and outlining strategies for infection control. There is, therefore, a need to ensure that the information being provided is of the highest standard and that any new technique is capable of delivering this.
Antimicrobial Susceptibility Testing Protocols
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Author : Richard Schwalbe
language : en
Publisher: CRC Press
Release Date : 2007-05-22
Antimicrobial Susceptibility Testing Protocols written by Richard Schwalbe and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-05-22 with Medical categories.
The clinical microbiology laboratory is often a sentinel for the detection of drug resistant strains of microorganisms. Standardized protocols require continual scrutiny to detect emerging phenotypic resistance patterns. The timely notification of clinicians with susceptibility results can initiate the alteration of antimicrobial chemotherapy and
Antibiotic Resistance Methods And Protocols
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Author : Stephen H. Gillespie
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-01
Antibiotic Resistance Methods And Protocols written by Stephen H. Gillespie and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-01 with Medical categories.
At a time of rising concern about drug resistance and falling output of new antibacterial compounds, antibiotic research has once again returned to the forefront of medical science. In Antibiotic Resistance: Methods and Protocols, Stephen Gillespie and a panel of leading clinical and diagnostic microbiologists describe a series of detailed molecular and physical methods designed to study the growing problem of antibiotic resistance, as well as facilitate new antibiotic research programs for its effective redress. The techniques range widely from those that provide rapid diagnosis via DNA amplification and phage display, to those for plotting the transmission of resistant organisms and investigating their epidemiology. The methods are readily adaptable to a wide range of resistant bacterial organisms. In order to ensure successful results, each method is described in minute detail and includes tips on avoiding pitfalls. Practical and wide-ranging, Antibiotic Resistance: Methods and Protocols provides a collection of indispensable techniques not only for illuminating the basic biology of antimicrobial resistance, but also for developing and implementing new diagnostic and epidemiological tools.
Molecular Mycobacteriology
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Author : Robert A. Ollar
language : en
Publisher: CRC Press
Release Date : 1999-01-04
Molecular Mycobacteriology written by Robert A. Ollar and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-01-04 with Science categories.
This work presents an overview of the clinical pathology of mycobacterial and nonpathogens, data on genetically based methodologies for clinical testing, straightforward protocols for molecular manipulations, and theoretical explanations of the molecular mechanisms involved. It explains the safest and most efficient methods for detecting mycobacterium avium complex, providing the means to combat this common secondary opportunistic infection in AIDS patients.
Antibiotic Resistance Protocols
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Author : Stephen H. Gillespie
language : en
Publisher:
Release Date : 2018
Antibiotic Resistance Protocols written by Stephen H. Gillespie and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with Antibiotics categories.
Microbial Steroids
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Author : Carlos Barreiro
language : en
Publisher: Springer Nature
Release Date : 2023-09-30
Microbial Steroids written by Carlos Barreiro and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-09-30 with Science categories.
This detailed volume explores experimental laboratory procedures for a wide range of steroid bioconversions. After an overview on the current trends and perspectives, the book continues with sections covering microbial screening and synthetic biology applied to microorganisms able to catabolize sterols, methods on strain characterization, including omics and biochemical analyses, methods of fermentation and biocatalysis for steroids production, as well as a chapter on the medical use of glucocorticoids in cancer patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Steroids: Methods and Protocols, Second Edition serves as an ideal reference source for laboratory and industrial professionals, as well as for students in a wide array of biological disciplines.
Antibiotic Resistance Protocols
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Author : Stephen H. Gillespie
language : en
Publisher: Humana
Release Date : 2010-04-16
Antibiotic Resistance Protocols written by Stephen H. Gillespie and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-04-16 with Medical categories.
This book fully updates and builds upon its first edition. Beginning with chapters on epidemiology and population genetics, it continues with sections covering genomics and gene expressions, fitness mutation and physiology, and the detection of resistance.