Nitric Oxide Protocols
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Nitric Oxide Protocols
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Author : M. A Titheradge
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Nitric Oxide Protocols written by M. A Titheradge and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
Michael Titheradge provides both the experienced researcher and the newcomer with time-tested techniques to cover all aspects of NO synthase and metabolism. Topics range from the cloning and expression of the different isoforms of NO synthase to the role of NO in DNA damage and apoptosis and include indirect and direct measurements of NO production, measurement on NO synthase mRNA both in situ and in vitro, and Western blotting and immunohistochemical localization of different isoforms of NO synthase. A variety of alternative techniques are described to enable researchers to set up an assay using either the simplest equipment or the most expensive EPR machines.
Nitric Oxide
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Author : Helen O. McCarthy
language : en
Publisher: Humana Press
Release Date : 2010-12-16
Nitric Oxide written by Helen O. McCarthy and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-12-16 with Science categories.
The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in vivo models remains challenging, due to the relatively unstable nature of the molecule. In Nitric Oxide: Methods and Protocols, experts in the field provide a detailed reference manual packed with step-by-step approaches to reproduce various complex protocols within your own laboratory. Divided into two parts, the volume first covers various disparate protocols relating to the direct detection and quantification of nitric oxide, including techniques which detail how to image real time in vivo generation of nitric oxide, quantify nitric oxide production in the rat brain, and the detect ultralow levels of nitric oxide in the pM range, and the book then continues with a section on techniques designed to either inhibit or enhance nitric oxide, with an aim to achieve therapeutic gain, featuring the inhibition of the nitric oxide synthase enzymes using viral, shRNA delivery systems to prevent cardiovascular dysfunction, peripheral neuropathy, and graft rejection. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Nitric Oxide: Methods and Protocols examines numerous complex methodologies detailed in each chapter and proves to be an invaluable resource for anyone with an interest in nitric oxide research.
Plant Nitric Oxide Methods And Protocols
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Author : Kapuganti Jagadis Gupta
language : en
Publisher: Methods in Molecular Biology
Release Date : 2018-05-05
Plant Nitric Oxide Methods And Protocols written by Kapuganti Jagadis Gupta and has been published by Methods in Molecular Biology this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-05 with Science categories.
With numerous methods available for the quantification of nitric oxide (NO), this detailed book explores their advantages and disadvantages in order to provide a foundation for further research in plant nitric oxide. After providing a useful practical guide to choosing a technique for measuring NO from plant materials, the book continues with chapters on chemiluminescence, diaminofluorescence (DAF), EPR spectroscopy, a laser-based method, as well as many other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory methods, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Nitric Oxide: Methods and Protocols serves as a valuable guide to all researchers working and intending to work in the field of plant nitric oxide research.
Nitric Oxide In Health And Disease
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Author : Jill Lincoln
language : en
Publisher: Cambridge University Press
Release Date : 1997-08-28
Nitric Oxide In Health And Disease written by Jill Lincoln and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-08-28 with Medical categories.
Nitric oxide has a tantalizing role in health and disease: while many of its wide-ranging effects are well known, there remains much more to explore and to learn about the interactions of this fascinating molecule in physiological and pathophysiological processes. The volume reviews the myriad effects of nitric oxide as a chemical messenger in the central nervous system, peripheral nervous system, immune system and cardiovascular system. Furthermore, it provides a very practical introduction to the procedures and experimental protocols necessary to work with and study nitric oxide and its synthesizing enzyme, nitric oxide synthase, in the laboratory. In this respect the volume is unique, providing as it does a complete single-volume review of the role of nitric oxide in health and disease, and a very practical introduction to the methods and protocols involved in this intriguing and active area of biomedical research.
Experimental Protocols For Reactive Oxygen And Nitrogen Species
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Author : Naoyuki Taniguchi
language : en
Publisher: OUP Oxford
Release Date : 2000-06-15
Experimental Protocols For Reactive Oxygen And Nitrogen Species written by Naoyuki Taniguchi and has been published by OUP Oxford this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-06-15 with Science categories.
Experimental Protocols for Reactive Oxygen and Nitrogen Species is a translated, expanded, and fully updated of the Japanese book Experimental Protocols for Reactive Oxygen Research: assay methods, gene analysis, and pathophysiology models published in 1994. The aim of the book is to provide experimental protocols covering many aspects of free radical research: biochemistry, molecular and cellular biology, genetics, physiology, and medicine. The protocols are all self-contained describing the equipment and reagents needed and then detailing the experimental procedure.
Nitric Oxide Protocols
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Author : Aviv Hassid
language : en
Publisher: Humana
Release Date : 2010-11-10
Nitric Oxide Protocols written by Aviv Hassid and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-10 with Science categories.
A collection of cutting-edge techniques for measuring nitric oxide and the enzyme that produces it in biological tissues and fluids. These readily reproducible methods can be used to measure novel nitric oxide-related products such as protein nitration and nitrosation, as well as to express nitric oxide synthase in basic research and gene therapy using viral vectors.
Nitric Oxide And The Regulation Of The Peripheral Circulation
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Author : Philip J. Kadowitz
language : en
Publisher: Springer Science & Business Media
Release Date : 2000
Nitric Oxide And The Regulation Of The Peripheral Circulation written by Philip J. Kadowitz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Medical categories.
Nitric oxide is involved in the regulation of the circulation in physiologic and pathophysiological conditions. Evidence indicates that alterations in endothelial production of nitric oxide may be involved in the pathogenesis of central hypertension, pulmonary hypertension, renal disease and coronary vasopastic disorders. In addition to being involved in regulation of the circulation in physiologic and pathophysiologic conditions, the inducible form of the enzyme may play a role in the refractory hypertension.
Who Guidelines For Indoor Air Quality
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Author :
language : en
Publisher: World Health Organization
Release Date : 2010
Who Guidelines For Indoor Air Quality written by and has been published by World Health Organization this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with House & Home categories.
This book presents WHO guidelines for the protection of public health from risks due to a number of chemicals commonly present in indoor air. The substances considered in this review, i.e. benzene, carbon monoxide, formaldehyde, naphthalene, nitrogen dioxide, polycyclic aromatic hydrocarbons (especially benzo[a]pyrene), radon, trichloroethylene and tetrachloroethylene, have indoor sources, are known in respect of their hazardousness to health and are often found indoors in concentrations of health concern. The guidelines are targeted at public health professionals involved in preventing health risks of environmental exposures, as well as specialists and authorities involved in the design and use of buildings, indoor materials and products. They provide a scientific basis for legally enforceable standards.
Nitric Oxide Protocols
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Author : Aviv Hassid
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Nitric Oxide Protocols written by Aviv Hassid and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Carrying on the high standards of the much-praised first edition of Nitric Oxide Protocols, Aviv Hassid has brought together a panel of expert researchers and clinician scientists to describe in step-by-step detail the latest methodologies for the measurement of nitric oxide--and the enzyme that produces it-in biological tissues and fluids. The authors take advantage of the latest methodologies for the quantitation of biological fluids and tissues, including capillary electrophoresis, microcoaxial electrodes, in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography, in situ hybridization, and real-time polymerase chain reaction. Chapters on the measurement of the novel products of nitric oxide, such as nitrated proteins, S-nitrosylated proteins, and dioxygen-dependent NO metabolism, are also included. Additional chapters address the expression of nitric oxide synthase via the use of viral vectors in gene therapy for erectile dysfunction and cancer, as well as in retrovirus, adenovirus, or adenoassociated virus-mediated expression of nitric oxide synthase in vivo. The protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Nitric Oxide Protocols, 2nd ed., offers investigators and clinican/scientists a gold-standard collection of readily reproducible analytical techniques for measuring levels of nitric oxide and determining its manifold functions and effects.
Nitric Oxide In Pulmonary Processes
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Author : Maria G. Belvisi
language : en
Publisher: Springer Science & Business Media
Release Date : 2000
Nitric Oxide In Pulmonary Processes written by Maria G. Belvisi and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Medical categories.
to Nitric Oxide Biology.- 1. Nitric Oxide Synthesis and Actions.- 2. Reactive Oxygen and Reactive Nitrogen Species in the Lung.- Role of Endogenous Nitric Oxide in the Lung.- 3. Non-Adrenergic Non-Cholinergic Neurotransmission in the Airways: Role of Nitric Oxide.- 4. Localisation of Nitric Oxide Synthases in the Lung.- 5. Role of Nitric Oxide in the Regulation of Pulmonary Vascular Tone.- 6. Nitric Oxide and Bronchial Hyperresponsiveness.- 7. Bronchodilator Actions of Nitric Oxide and Related Compounds.- 8. Role of Nitric Oxide in Airway Inflammation.- Therapeutic Potential of Inhalded Nitric Oxide and Nitric Oxide Synthase Inhibitors in Lung Disease.- 9. Nitric Oxide in Exhaled Air: Relevance in Inflammatory Lung Disease.- 10. Luminal Nitric Oxide in the Upper Airways: Implications for Local and Distal Sites of Action.- 11. Inhaled Nitric Oxide as a Therapy for Diseases of the Pulmonary Vasculature.- 12. Combinded Use of Nitric Oxide and Nitric Oxide Synthase Inhibitors as a Possible Therapeutic Approach.