Nuclear Transfer Protocols
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Nuclear Transfer Protocols
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Author : Paul J. Verma
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Nuclear Transfer Protocols written by Paul J. Verma and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the gene- tion of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and serial nuclear transfer. Of immense practical benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis also provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included making this an essential comprehensive handbook for research and commercial labo- tories involved in, or intending to work on, nuclear transfer. The volume will prove beneficial to molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.
Nuclear Transfer Protocols
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Author : Paul J. Verma
language : en
Publisher:
Release Date : 2006
Nuclear Transfer Protocols written by Paul J. Verma and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with categories.
"Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis" is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and post-fusion enucleation nuclear transfer.
Nuclear Reprogramming
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Author : Kejin Hu
language : en
Publisher: Humana
Release Date : 2021-12-08
Nuclear Reprogramming written by Kejin Hu and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-12-08 with Science categories.
This volume provides basic and advanced protocols on somatic cell nuclear transfer, induced pluripotent stem cells, and direct reprogramming of somatic cells into different functional cells. Chapters guide readers through methods on standardized procedures for characterization of induced pluripotent stem cells, as well as those for preparation of materials required for induction of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nuclear Reprogramming: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Somatic Cell Nuclear Transfer Technology
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Author : Marcelo Tigre Moura
language : en
Publisher: Humana
Release Date : 2023-05-07
Somatic Cell Nuclear Transfer Technology written by Marcelo Tigre Moura and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-05-07 with Science categories.
This volume details protocols on animal cloning by Somatic cell nuclear transfer for basic research and biotechnological applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Somatic Cell Nuclear Transfer Technology aims to be comprehensive guide for researchers.
Nuclear Transfer Protocols
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Author : Paul J. Verma
language : en
Publisher: Humana Press
Release Date : 2006-07-18
Nuclear Transfer Protocols written by Paul J. Verma and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-07-18 with Science categories.
Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the gene- tion of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and serial nuclear transfer. Of immense practical benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis also provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included making this an essential comprehensive handbook for research and commercial labo- tories involved in, or intending to work on, nuclear transfer. The volume will prove beneficial to molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.
Nuclear Reprogramming
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Author : Elijah Joshua
language : en
Publisher: Createspace Independent Publishing Platform
Release Date : 2017-06-12
Nuclear Reprogramming written by Elijah Joshua and has been published by Createspace Independent Publishing Platform this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-06-12 with categories.
Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised collection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Nuclear Reprogramming
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Author : Kejin Hu
language : en
Publisher:
Release Date : 2021
Nuclear Reprogramming written by Kejin Hu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021 with Cell nuclei categories.
This volume provides basic and advanced protocols on somatic cell nuclear transfer, induced pluripotent stem cells, and direct reprogramming of somatic cells into different functional cells. Chapters guide readers through methods on standardized procedures for characterization of induced pluripotent stem cells, as well as those for preparation of materials required for induction of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nuclear Reprogramming: Methods and Protocols aims to ensure successful results in the further study of this vital field. .
Cell Reprogramming
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Author : Paul J. Verma
language : en
Publisher: Humana
Release Date : 2015-12-02
Cell Reprogramming written by Paul J. Verma and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-12-02 with Science categories.
This volume provides an understanding of the factors involved in nuclear reprogramming, which is essential for the success of reprogramming. The book is aimed at reprogramming differentiated cells and germ line transmission of pluripotent stem cells and features chapters that deal with reprogramming-related issues such as analysis of mitochondrial DNA in reprogrammed cells and the isolation of reprogramming intermediates; alternative methods for nuclear transfer; the production of germ-line chimeras from embryonic stem cells and induced pluripotent stem cells; and neonatal care and management of somatic cell nuclear transfer derived offspring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell Reprogramming: Methods and Protocols
Nuclear Reprogramming
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Author : Steve Pells
language : en
Publisher: Humana
Release Date : 2005-12-05
Nuclear Reprogramming written by Steve Pells and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-12-05 with Science categories.
A wide-ranging collection of readily reproducible methods for performing nuclear reprogramming by nuclear transfer in several different species, by fusion through both chemical treatment and electrically shocking cells, and by in vivo treatment of cells with cell extracts. Several methods of monitoring nuclear reprogramming are also presented, including the use of transgenic markers, activation of telomerase as an ES-specific marker, light and electron microscopic observation of structural changes in the nucleus, and verification of surface marker expression and the differentiation potential of stem cells. Biochemical methods are provided for the examination of chromatin protein modifications, nucleosomal footprinting, transcription factor binding, and the study of DNA methylation changes both at the specific locus level and at the level of the whole nucleus.
Bovine Reproduction
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Author : Richard M. Hopper
language : en
Publisher: John Wiley & Sons
Release Date : 2014-08-18
Bovine Reproduction written by Richard M. Hopper and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-08-18 with Medical categories.
Bovine Reproduction is a comprehensive, current reference providing information on all aspects of reproduction in the bull and cow. Offering fundamental knowledge on evaluating and restoring fertility in the bovine patient, the book also places information in the context of herd health where appropriate for a truly global view of bovine theriogenology. Printed in full color throughout, the book includes 83 chapters and more than 550 images, making it the most exhaustive reference available on this topic. Each section covers anatomy and physiology, breeding management, and reproductive surgery, as well as obstetrics and pregnancy wastage in the cow. Bovine Reproduction is a welcome resource for bovine practitioners, theriogenologists, and animal scientists, as well as veterinary students and residents with an interest in the cow.