Optical Spectroscopic And Microscopic Techniques


Optical Spectroscopic And Microscopic Techniques
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Optical Spectroscopic And Microscopic Techniques


Optical Spectroscopic And Microscopic Techniques
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Author : Harekrushna Sahoo
language : en
Publisher:
Release Date : 2022

Optical Spectroscopic And Microscopic Techniques written by Harekrushna Sahoo and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022 with categories.


This book illustrates the significance of various optical spectroscopy and microscopy techniques, including absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and Raman spectroscopy for deciphering the nature of biological molecules. The content of this book chiefly focuses on (1) the principle, theory, and instrumentation used in different optical spectroscopy techniques, and (2) the application of these techniques in exploring the nature of different biomolecules (e.g., proteins, nucleic acids, enzymes, and carbohydrates). It emphasizes the structural, conformational and dynamic, and kinetic including the changes in biomolecules under a range of conditions. In closing, the book summarizes recent advances in the field of optical spectroscopic and microscopic techniques.



Optical Microscopic And Spectroscopic Techniques Targeting Biological Applications


Optical Microscopic And Spectroscopic Techniques Targeting Biological Applications
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Author : Vicente Micó
language : en
Publisher: Frontiers Media SA
Release Date : 2021-10-22

Optical Microscopic And Spectroscopic Techniques Targeting Biological Applications written by Vicente Micó and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-10-22 with Science categories.




Optical Imaging And Microscopy


Optical Imaging And Microscopy
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Author : Peter Török
language : en
Publisher: Springer
Release Date : 2013-06-05

Optical Imaging And Microscopy written by Peter Török and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-05 with Science categories.


This text draws together the fields of optical microscopy and optical data storage, in a unique compilation of valuable and novel scientific work that is scarcely to be found elsewhere. The contributing authors are unquestioned leaders of their respective fields.



New Techniques Of Optical Microscopy And Microspectroscopy


New Techniques Of Optical Microscopy And Microspectroscopy
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Author : Cherry
language : en
Publisher: CRC Press
Release Date : 1991

New Techniques Of Optical Microscopy And Microspectroscopy written by Cherry and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with Science categories.


The last few years have seen an upsurge of interest in the study of cells by optical microscopy. The advent of new techniques such as confocal microscopy and the availability of extremely sensitive digital imaging devices are revolutioniz-ing the field. A number of groups have developed new ways of making spectroscopic measurements at the microscopic level, accompanied by the introduction of appropriate sen-sor molecules for cellular assays. The aim of this volume will be to bring together the various advances in order to provide the reader with an up-to-date account of what can now be achieved with modern optical microscopic methods.



Optical Spectroscopy


Optical Spectroscopy
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Author : Nikolai V. Tkachenko
language : en
Publisher: Elsevier
Release Date : 2006-06-06

Optical Spectroscopy written by Nikolai V. Tkachenko and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-06-06 with Science categories.


Optical Spectroscopy bridges a gap by providing a background on optics while focusing on spectroscopic methodologies, tools and instrumentations. The book introduces the most widely used steady-state and time-resolved spectroscopic techniques, makes comparisions between them, and provides the methodology for estimating the most important characteristics of the techniques such as sensitivity and time resolution. Recent developments in lasers, optics and electronics has had a significant impact on modern optical spectroscopic methods and instrumentations. Combining the newest lasers, advanced detectors and other high technology components researchers are able to assemble a spectroscopic instrument with characteristics that were hardly achievable a decade ago. This book will help readers to sourse spectroscopy tools to solve their problems by providing information on the most widely used methods while introducing readers to the principles of quantitative analysis of the application range for each methodology. In addition, background information is provided on optics, optical measurements and laser physics, which is of crucial importance for spectroscopic applications. * provides an overview of the most popular absorption/emission spectroscopy techniques* discusses application range, advantages and disadvantages are compared for different spectroscopy methods* provides introductions to the relevant topics such as optics and laser physics



Superresolution Optical Microscopy


Superresolution Optical Microscopy
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Author : Barry R. Masters
language : en
Publisher: Springer Nature
Release Date : 2020-03-21

Superresolution Optical Microscopy written by Barry R. Masters and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-03-21 with Science categories.


This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.



Optical Spectroscopic And Microscopic Techniques


Optical Spectroscopic And Microscopic Techniques
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Author : Harekrushna Sahoo
language : en
Publisher: Springer Nature
Release Date : 2022-02-25

Optical Spectroscopic And Microscopic Techniques written by Harekrushna Sahoo and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-02-25 with Science categories.


This book illustrates the significance of various optical spectroscopy and microscopy techniques, including absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and Raman spectroscopy for deciphering the nature of biological molecules. The content of this book chiefly focuses on (1) the principle, theory, and instrumentation used in different optical spectroscopy techniques, and (2) the application of these techniques in exploring the nature of different biomolecules (e.g., proteins, nucleic acids, enzymes, and carbohydrates). It emphasizes the structural, conformational and dynamic, and kinetic including the changes in biomolecules under a range of conditions. In closing, the book summarizes recent advances in the field of optical spectroscopic and microscopic techniques.



Surface And Interface Characterization By Electron Optical Methods


Surface And Interface Characterization By Electron Optical Methods
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Author : Ugo Valdre
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-09

Surface And Interface Characterization By Electron Optical Methods written by Ugo Valdre and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-09 with Science categories.


The importance of real space imaging and spatially-resolved spectroscopy in many of the most significant problems of surface and interface behaviour is almost self evident. To join the expertise of the tradi tional surface scientist with that of the electron microscopist has however been a slow and difficult process. In the past few years remarkable progress has been achieved, including the development of new techniques of scanning transmission and reflection imaging as well as low energy microscopy, all carried out in greatly improved vacuum conditions. Most astonishing of all has been the advent of the scanning tunneling electron microscope providing atomic resolution in a manner readily compatible with most surface science diagnostic procedures. The problem of beam damage, though often serious, is increasingly well understood so that we can assess the reliability and usefulness of the results which can now be obtained in catalysis studies and a wide range of surface science applications. These new developments and many others in more established surface techniques are all described in this book, based on lectures given at a NATO Advanced Study Institute held in Erice, Sicily, at Easter 1987. It is regretted that a few lectures on low energy electron diffraction and channeling effects could not be included. Fifteen lecturers from seven different Countries and 67 students from 23 Countries and a wide variety of backgrounds attended the school.



Near Field Coherent Optical Spectroscopy And Microscopy Of A Mesoscopic Quantum System


Near Field Coherent Optical Spectroscopy And Microscopy Of A Mesoscopic Quantum System
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Author : Jeffrey Randall Guest
language : en
Publisher:
Release Date : 2000

Near Field Coherent Optical Spectroscopy And Microscopy Of A Mesoscopic Quantum System written by Jeffrey Randall Guest and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with categories.




Optical Techniques In Biological Research


Optical Techniques In Biological Research
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Author : Denis L. Rousseau
language : en
Publisher:
Release Date : 1984

Optical Techniques In Biological Research written by Denis L. Rousseau and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Science categories.


Light scattering / Bennie R. Ware -- Raman scattering / Denis L. Rousseau and Mark R. Ondrias -- Fourier transform infrared spectroscopy / James O. Alben and Frank G. Fiamingo -- Nanosecond fluorescence spectroscopy / Juan Yguerabide and Evangelina E. Yguerabide -- Circular dichroism / Marcos F. Maestre -- Electron microscopy / S.W. Hui.