Patch Clamp And Single Cell Voltage Clamp Techniques
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Patch Clamp Methods And Protocols
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Author : Peter Molnar
language : en
Publisher: Springer Science & Business Media
Release Date : 2007
Patch Clamp Methods And Protocols written by Peter Molnar and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Medical categories.
Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described require a basic level of electrophysiological training and aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. Patch Clamp Methods and Protocols is divided into three sections that cover the major areas of patch clamp application: Pharmacology, Physiology, and Biophysics. The first section provides examples and step by step instructions on how to use whole-cell and single-channel patch clamp methods for testing drugs in industrial settings. The second section provides a wide selection of patch clamp applications in physiological studies. The last part focuses on the biophysical applications of the patch clamp method using single channel recordings or statistical analysis of whole-cell currents in order to obtain parameters that describe ion channel properties or transmitter release. Individual techniques are explored within the area that they are applied most often. Researchers will find Patch Clamp Methods and Protocols to be an invaluable aid in the design and execution of a wide variety of patch clamp experiments, both on their own and in conjunction with other state-of-the-art methodologies.
Patch Clamp And Single Cell Voltage Clamp Techniques
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Author :
language : en
Publisher:
Release Date : 1988
Patch Clamp And Single Cell Voltage Clamp Techniques written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with categories.
Patch Clamp And Single Cell Voltage Clamp Techniques
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Author : Nick Sperelakis
language : en
Publisher:
Release Date : 1988
Patch Clamp And Single Cell Voltage Clamp Techniques written by Nick Sperelakis and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with Voltage-clamp techniques (Electrophysiology) categories.
Patch Clamping
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Author : Areles Molleman
language : en
Publisher: John Wiley & Sons
Release Date : 2003-06-13
Patch Clamping written by Areles Molleman and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-06-13 with Science categories.
Patch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells. Patch Clamping aims to cover the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the basic principles, platforms, equipment and environmental control, and will also include coverage of preparation types, recording modes and analysis of results. This book will explain the basic principles and practical application of patch clamp electrophysiology Written in a non-technical style to ensure its broad appeal to novice users Takes a practical approach This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this technique, including an overview of membrane biophysics, standard experimental design, data analysis, and technical concerns
Voltage And Patch Clamping With Microelectrodes
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Author : Thomas G Smith
language : en
Publisher: Springer
Release Date : 2013-05-27
Voltage And Patch Clamping With Microelectrodes written by Thomas G Smith and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-05-27 with Medical categories.
Edited and authored by international experts on voltage and patch clamping, this volume is designed to help anyone undertaking experiments requiring the use of these techniques. The only book of its kind to bring together this wealth of information on theory as well as practical techniques, this is a volume that no one involved in voltage and patch clamping can afford to be without.
Patch Clamp Analysis
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Author : Wolfgang Walz
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-06
Patch Clamp Analysis written by Wolfgang Walz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-06 with Medical categories.
Patch-Clamp Analysis: Advanced Techniques describes in reproducible detail all applications that involve patch pipet. Beginning with updated basic patch-clamp techniques, the book presents the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose patch, the perforated patch, and the patch cram detection technique. The advanced techniques covered combine molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques.
Patch Clamp Techniques
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Author : Yasunobu Okada
language : en
Publisher: Springer
Release Date : 2012-04-27
Patch Clamp Techniques written by Yasunobu Okada and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-04-27 with Medical categories.
Channels and transporters are multi-functional proteins that mediate substrate transport and signal transmission and simultaneously act as regulators for other proteins and biosensors for environmental materials. Patch clamping is an epoch-making technique that allows researchers to perform real-time measurements of electrogenic channel/transporter functions at the single/multiple molecular level. This book describes not only the conventional patch clamp techniques but also their newly developed variations or applications, such as perforated patch, slice patch, blind patch, in vivo patch, imaging patch, smart patch, and automated patch clamping. These patch clamp techniques are now essential and are extensively used across the life sciences and in related industries. With plain and practical descriptions of patch clamping and how to carry it out, especially for beginners, the book also shows how widely and exquisitely the patch clamp techniques can be applied by expert electrophysiologists. This work serves as a useful guide for young researchers and students in training and laboratory courses as well as for senior researchers who wish to extend their repertoire of techniques.
Patch Clamp Technique
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Author : Fatima Shad Kaneez
language : en
Publisher: BoD – Books on Demand
Release Date : 2012-03-23
Patch Clamp Technique written by Fatima Shad Kaneez and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-03-23 with Science categories.
This book is a stimulating and interesting addition to the collected works on Patch clamp technique. Patch Clamping is an electrophysiological technique, which measures the electric current generated by a living cell, due to the movement of ions through the protein channels present in the cell membrane. The technique was developed by two German scientists, Erwin Neher and Bert Sakmann, who received the Nobel Prize in 1991 in Physiology for this innovative work. Patch clamp technique is used for measuring drug effect against a series of diseases and to find out the mechanism of diseases in animals and plants. It is also most useful in finding out the structure function activities of compounds and drugs, and most leading pharmaceutical companies used this technique for their drugs before bringing them for clinical trial. This book deals with the understanding of endogenous mechanisms of cells and their receptors as well as advantages of using this technique. It covers the basic principles and preparation types and also deals with the latest developments in the traditional patch clamp technique. Some chapters in this book take the technique to a next level of modulation and novel approach. This book will be of good value for students of physiology, neuroscience, cell biology and biophysics.
Signal Transduction Single Cell Techniques
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Author : Bert, van Duijn
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Signal Transduction Single Cell Techniques written by Bert, van Duijn and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
A variety of powerful techniques for monitoring and analysing events during signal transduction at the single cell level are described in this lab manual. An introductionary section on cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH.
Single Channel Recording
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Author : Bert Sakmann
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-10-03
Single Channel Recording written by Bert Sakmann and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-10-03 with Science categories.
Edited by the 1991 winners of the Nobel Prize in Physiology or Medicine, this Second Edition includes new chapters covering such applications as capacitance measurements; single-cell PCR measurements; whole-cell recording from brain slices in combination with imaging techniques; atomic force microscopy of cells and membranes attached to glass pipettes; and patch clamping.