Pcr Methods Express

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Pcr Methods Express
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Author : Simon Hughes
language : en
Publisher: Scion Publishing Ltd
Release Date : 2007-05-01
Pcr Methods Express written by Simon Hughes and has been published by Scion Publishing Ltd this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-05-01 with Science categories.
PCR is the most widely used technique in molecular biology. New PCR variants offering substantial benefits to existing protocols appear on a frequent basis. PCR: Methods Express describes the very latest PCR-based methodologies and approaches to provide the most up-to-date practical advice on how to tackle a broad range of biological problems including: *real time qRT-PCR *rapid generation of gene targeting constructs *PCR multiplexes *PCR-based mutagenesis *identification of microdeletions and microduplications *DNA methylation analysis *forensic genetic DNA typing *genotyping *identification of mutations in single cells *whole genome amplification *diagnosis of infectious diseases *inverse PCR-based RFLP This book is a comprehensive research guide; every chapter discusses the merits and limitations of the available approaches and then provides fully-proven protocols with hints and tips for success. PCR: Methods Express is an essential laboratory manual for researchers in all life science fields and at all levels, from postgraduate student to principal investigator.
Pcr Methods Express
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Author : Hughes S.
language : en
Publisher:
Release Date : 2007
Pcr Methods Express written by Hughes S. and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with categories.
Poisoning
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Author : Ntambwe Malangu
language : en
Publisher: BoD – Books on Demand
Release Date : 2017-12-20
Poisoning written by Ntambwe Malangu and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-20 with Medical categories.
This book, which is the result of contributions from a team of international authors, presents a collection of materials that can be categorized into two groups. The first group of papers deals with clinical toxicology topics including poisoning by anticoagulant rodenticides, food toxins, carbon monoxide, the toxicity of beta-lactam antibiotics, acute neonicotinoid poisoning, occupational risk factors for acute pesticide poisoning, activating carbon fibers, and date pits for use in liver toxin adsorption. The second group of papers deals with forensic or analytical toxicology topics such as simplified methods for the analysis of gaseous toxic agents, rapid methods for the analysis and monitoring of pathogens in drinking water and water-based solutions, as well as the linkages between clinical and forensic toxicology. Each chapter presents new information on the topic discussed based on authors' experience while summarizing existing knowledge. As such, this book will be a good teaching aid and can be a prescribed or recommended reading for postgraduate students and professionals in the fields of public health, medicine, pharmacy, nursing, biology, toxicology, and forensic sciences.
Dna Techniques To Verify Food Authenticity
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Author : Malcolm Burns
language : en
Publisher: Royal Society of Chemistry
Release Date : 2019-10-14
Dna Techniques To Verify Food Authenticity written by Malcolm Burns and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-10-14 with Technology & Engineering categories.
The food supply chain needs to reassure consumers and businesses about the safety and standards of food. Global estimates of the cost of food fraud to economies run into billions of dollars hence a huge surge in interest in food authenticity and means of detecting and preventing food fraud and food crime. Approaches targeting DNA markers have assumed a pre-eminence. This book is the most comprehensive and timely collection of material from those working at the forefront of DNA techniques applied to food authenticity. Addressing the new field of analytical molecular biology as it combines the quality assurance rigour of analytical chemistry with DNA techniques, it introduces the science behind DNA as a target analyte, its extraction, amplification, detection and quantitation as applied to the detection of food fraud and food crime. Making the link with traditional forensic DNA profiling and describing emerging and cutting-edge techniques such as next generation sequencing, this book presents real-world case studies from a wide perspective including from analytical service providers, industry, enforcement agencies and academics. It will appeal to food testing laboratories worldwide, who are just starting to use these techniques and students of molecular biology, food science and food integrity. Food policy professionals and regulatory organisations who will be using these techniques to back up legislation and regulation will find the text invaluable. Those in the food industry in regulatory and technical roles will want to have this book on their desks.
Essentials Of Nucleic Acid Analysis
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Author : Jacquie T Keer
language : en
Publisher: Royal Society of Chemistry
Release Date : 2008-02-25
Essentials Of Nucleic Acid Analysis written by Jacquie T Keer and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-25 with Science categories.
Over the last decade there has been a rapid development of molecular techniques, with an increasing range of instrumentation now available. The development of accompanying reference literature has not kept pace with technological advances and this poses significant challenges to the analyst. Essentials of Nucleic Acid Analysis sets out to guide the analyst through the steps needed to obtain good quality results in DNA analysis. The underlying principles for achieving this goal were formulated by LGC (formerly the Laboratory of the Government Chemist) as the six principles for ensuring valid analytical measurement, which are detailed in the introduction. The reader is also provided with guidelines for method validation and quality control of established and emerging DNA measurement techniques. The authors of each chapter are practitioners of the art of DNA analysis in areas where the quality of the result is critical. Technical details and examples of application of key techniques in nucleic acid analysis are provided while highlighting best practice, available standards and practical advice on improving measurement quality. This book provides an indispensable handbook and premier reference for those working in the widely varying areas and specifically in the fields of food analysis and forensic applications.
Pcr Protocols
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Author : John M. S. Bartlett
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Pcr Protocols written by John M. S. Bartlett and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
Genome Research
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Author :
language : en
Publisher:
Release Date : 2007
Genome Research written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with categories.
Cellular And Molecular Methods In Neuroscience Research
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Author : Adalberto Merighi
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-12-14
Cellular And Molecular Methods In Neuroscience Research written by Adalberto Merighi and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-14 with Medical categories.
There are numerous books on cellular and molecular protocols for general use in cell biology but very few are exclusively devoted to neurobiology. This book fills this gap and explains in a clear and consistent manner, some of the more commonly used protocols in neuroscience research. Each chapter is written by either the person who invented the procedure or an expert in the field. The format is uniform: "Overview," "Background," "Protocols," and "results and discussion." Each protocol begins with the principle of the technique, studies in cell culture, materials and reagents, and, lastly, step-by-step outline of the procedure itself. This highly practical book is also well illustrated (with 17 four color plates) to make the concepts and procedures easy to understand and perform.
Pcr
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Author : Simon Hughes
language : en
Publisher: Methods Express (Hardcover)
Release Date : 2007
Pcr written by Simon Hughes and has been published by Methods Express (Hardcover) this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Science categories.
"PCR: Methods Express" describes the very latest PCR-based methodologies and approaches to provide the most up-to-date practical advice on how to tackle a broad range of biological problems.
Textbook Of Immunology
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Author : Constantin A. Bona
language : en
Publisher: CRC Press
Release Date : 2019-11-11
Textbook Of Immunology written by Constantin A. Bona and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-11-11 with Medical categories.
Building on the strengths of the first edition, the newly titled and expanded second edition remains a concise introduction to the fundamentals of immunology, with an expert synthesis of basic and clinical information., Augmented by color illustrations, and with increased emphasis on the molecular and genetic underpinnings of cellular phenomena, Textbook of Immunology covers the physiology of the immune system, disease entities related to immune system dysfunction, and the underlying pathophysiologic mechanisms of dysfunction. In response to advancing knowledge that influences the approach to presenting basic immunology, new chapters have been added on cytokines; host defense (non-specific immunity and specific immune responses); the aging immune system; and the pathophysiology, diagnosis, prevention, and therapy of AIDS., This book keeps pace with the explosion of information and data in immunology, and adeptly refines, organizes, and presents this body of knowledge to serve as a succinct introduction to modern immunologic concepts for medical students, and as an update and refresher in the basics for researchers and clinicians.