Plant Microtechniques And Protocols


Plant Microtechniques And Protocols
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Plant Microtechniques And Protocols


Plant Microtechniques And Protocols
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Author : Edward Chee Tak Yeung
language : en
Publisher: Springer
Release Date : 2015-09-19

Plant Microtechniques And Protocols written by Edward Chee Tak Yeung and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-09-19 with Science categories.


A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.​



Plant Microtechniques And Protocols


Plant Microtechniques And Protocols
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Author : Edward Chee Tak Yeung
language : en
Publisher:
Release Date : 2015

Plant Microtechniques And Protocols written by Edward Chee Tak Yeung and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.


This work gathers common plant microtechniques and updates their procedures using a simple and fully understandable approach. The text serves as a handy resource for scientists familiar with the protocols and as a guide for the novice, especially students just beginning to learn about various structural methods for the first time. This book analyzes a range of topics in order to generate cross-talks among scientists in different research disciplines. The first section of this volume covers the more commonly used embedding methods, with emphasis on the preparative methods for light and electron microscopy. A number of cell biology related protocols are compiled in the second section to showcase the usefulness of various techniques based on different processing and staining methods. The third section highlights some common and recent procedures in wood preparation. The last section includes botanical methods related to archaeological uses of plant materials. A special chapter on field and herbarium procedures is also included to serve as a guide to students interested in plant collection and taxonomic studies.



Plant Cell Culture Protocols


Plant Cell Culture Protocols
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Author : Víctor M. Loyola-Vargas
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04

Plant Cell Culture Protocols written by Víctor M. Loyola-Vargas and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.


A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.



Plant Tissue Culture


Plant Tissue Culture
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Author : B. N. Sathyanarayana
language : en
Publisher: I. K. International Pvt Ltd
Release Date : 2007

Plant Tissue Culture written by B. N. Sathyanarayana and has been published by I. K. International Pvt Ltd this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Botany, Experimental categories.


Plant Tissue Culture forms an integral basis of the present day biotechnology. Plant Tissue Culture: Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.



Plant Microtechnique


Plant Microtechnique
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Author : D. A. Jahansen
language : en
Publisher:
Release Date : 1979

Plant Microtechnique written by D. A. Jahansen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1979 with categories.




Plant Microtechnique And Microscopy


Plant Microtechnique And Microscopy
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Author : Steven E. Ruzin
language : en
Publisher: Oxford University Press, USA
Release Date : 1999

Plant Microtechnique And Microscopy written by Steven E. Ruzin and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Science categories.


Plant microtechnique has generated renewed interest in recent years, due in part to the need for molecular biologists to visualize a gene or gene product in the context of the whole plant. Plant Microtechnique and Microscopy offers uniquely in-depth coverage of this reinvigorated field. Thoroughly covering classical aspects of microscope slide preparation, it goes a step beyond all other available manuals by also documenting the theory and practice of modern applications. The text opens with single-page "Quick Start" protocols that provide students with fundamental instructions to complete eight of the most common microtechnique protocols used today. The following sections cover the theory and practice of microtechnique. The traditional paraffin method is demonstrated by explicit step-by-step protocols, and theoretical background is incorporated to give students the tools required to design their own experiments and to interpret existing results. In addition, modern applications such as methacrylate embedding and sectioning, microwave tissue processing, fluorescence histochemistry, and in situ hybridization are discussed in detail. The manual also contains a definitive chapter on microscopy and describes, in both text and diagrams, the optical principles of techniques such as phase contrast and DIC as well as confocal and deconvolution wide-field microscopy. Appendices on laboratory practice (chemical toxicities, common calculations, and buffer tables), an extensive appendix on optics and its application to the microscope, and an extensive bibliography of over 550 references are also included. Ideal for courses in plant biology, Plant Microtechnique and Microscopy also serves as an indispensable reference for all students of microscopy, histology, and histological technique. It is a valuable addition to every biological laboratory.



Plant Tissue Culture New Techniques And Application In Horticultural Species Of Tropical Region


Plant Tissue Culture New Techniques And Application In Horticultural Species Of Tropical Region
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Author : Duong Tan Nhut
language : en
Publisher: Springer Nature
Release Date : 2022-04-05

Plant Tissue Culture New Techniques And Application In Horticultural Species Of Tropical Region written by Duong Tan Nhut and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-04-05 with Science categories.


This book presents latest work in the field of plant biotechnology regarding high-efficiency micropropagation for commercial exploitation at low labor and equipment costs. The book consists of 18 chapters on establishing advanced culture systems, techniques as well as latest modification protocols on a variety of crops. It also discusses new methods such as nylon film culture system, light-emitting diode and wireless light-emitting diode system, stem elongation, wounding manipulation and shoot tip removal, in vitro hydroponic and microponic culture system, thin cell layer culture system etc. Plant cell tissue has been developed more than fifty years ago. Since then applications of in vitro plant propagation expanded rapidly all around the world and played as an important role in agricultural and horticultural systems. This book will be of interest to teachers, researchers, scientists, capacity builders and policymakers. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences.



The Plant Cell Wall


The Plant Cell Wall
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Author : Zoë A. Popper
language : en
Publisher:
Release Date : 2020

The Plant Cell Wall written by Zoë A. Popper and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020 with Plant cell walls categories.




Protocols For Macroalgae Research


Protocols For Macroalgae Research
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Author : Bénédicte Charrier
language : en
Publisher: CRC Press
Release Date : 2018-04-17

Protocols For Macroalgae Research written by Bénédicte Charrier and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-17 with Medical categories.


This book presents a wide range of tested and proven protocols relevant to a number of fields within biotechnology used in laboratory experiments in everyday phycological (seaweed) research. A major focus will be on bioenergy related aspects of this emerging technology. These protocols will be written in a clear and concise manner using simple language permitting even nonspecialist to adequately understand the significance of this research. It will also contain all necesssary notes and guidelines for successful execution of these experiments.



Orchid Propagation


Orchid Propagation
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Author : Yung-I. Lee
language : en
Publisher:
Release Date : 2018

Orchid Propagation written by Yung-I. Lee and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with Orchids categories.