Polymerase Chain Reaction Pcr
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Pcr Topics
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Author : Arndt Rolfs
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-12-01
Pcr Topics written by Arndt Rolfs and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-01 with Medical categories.
PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary Mullis and Randall K. Saiki, is a very simple method for amplifying nucleic acids in vitro. The realization of this idea bases on the repetition of a set of three different temperatures and yields an increase of the target structure up to a factor of 106 to 1012. Therefore, this technique is predisposed for safe analysis and characterization of DNA and RNA sequences of interest, even where the starting amount of material is enormously small. Because of its sensitivity, speed and versatility this method is particularly suitable for investigations of oncogenes, tumor associated translocations, retroviral sequences, lymphokines and mainly the broad field of degenerative and inflammatory diseases of nervous system. PCR seems to be the technique which could overcome the two most important problems in that field: very small amount of material combined with the necessity of rapid diagnostic procedures in inflammatory infections. "PCR topics" will give an actual overview of basic and applied research fields on usage of polymerase chain reaction. All contributions to this book have been presented at an international congress on "Usage of Polymerase chain reaction in genetic and infectious diseases" which took place in june 1990 in Berlin. The editors wish to thank all participants for their contributions. We offer our thanks and gratitude to our coworkers and especially to our technical assistents Barbara Trampenau, Mirjana Wiirdemann and Hannelore Leonhard.
The Polymerase Chain Reaction
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Author : Kary B. Mullis
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-02-02
The Polymerase Chain Reaction written by Kary B. Mullis and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-02-02 with Medical categories.
James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...
Polymerase Chain Reaction
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Author : Shehnam Shafique
language : en
Publisher: Lulu.com
Release Date : 2012-05-05
Polymerase Chain Reaction written by Shehnam Shafique and has been published by Lulu.com this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-05-05 with Science categories.
This PCR handbook establishes an easy approach for the students going through their studies in Molecular Diagnostics. It introduces timely topics such as Clinical Applications of PCR, Real time PCR, PCR Optimization, Application of PCR, PCR Array System Performance, Designing the primers, PCR in diagnosis of diseases and different PCR Techniques and their procedures.
Polymerase Chain Reaction Pcr
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Author : Rosalind A. Eeles
language : en
Publisher:
Release Date : 1993
Polymerase Chain Reaction Pcr written by Rosalind A. Eeles and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Polymerase chain reaction categories.
Pcr Protocols
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Author : Bruce A. White
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Pcr Protocols written by Bruce A. White and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.
The Polymerase Chain Reaction Pcr For Human Viral Diagnosis
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Author : Jonathan P. Clewley
language : en
Publisher: CRC Press
Release Date : 1994-10-27
The Polymerase Chain Reaction Pcr For Human Viral Diagnosis written by Jonathan P. Clewley and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-10-27 with Science categories.
The polymerase chain reaction (PCR) is the most sensitive method for revealing the presence of otherwise undetectable quantities of the genome of RNA or DNA of human viruses. The Polymerase Chain Reaction (PCR) for Human Viral Diagnosis addresses the urgent need to use this revolutionary technology in reference and routine diagnostic laboratories. It informs the molecular biologist of the most appropriate clinical uses for PCR and educates the clinician and medical virologist about the subtleties and benefits of gene amplification. The reader is given an understanding and appreciation of the principles of PCR and how, why, and where it should be applied. The book explains the principles behind PCR and its role in the diagnostic and public health laboratory. The application of PCR to the detection and investigation of viral latency and persistence is presented by the originators of in situ amplification. There are individual contributions from experts in their respective fields on the detection, characterization, and analysis by PCR of gastroenteritis viruses, hepatitis viruses, herpesviruses, rhinoviruses, enteroviruses, flaviviruses, polyomaviruses, human immunodeficiency virus (HIV), human T-lymphotropic virus types I and II (HTLV-I and II); and measles, mumps, rubella, influenza, rabies, and B19 viruses.
Pcr Protocols
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Author : John M. S. Bartlett
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Pcr Protocols written by John M. S. Bartlett and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
Polymerase Chain Reaction
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Author : Azra Hasan
language : en
Publisher: Elsevier Health Sciences
Release Date : 2019-10-31
Polymerase Chain Reaction written by Azra Hasan and has been published by Elsevier Health Sciences this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-10-31 with Medical categories.
Polymerase Chain Reaction
Polymerase Chain Reaction For Biomedical Applications
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Author : Ali Samadikuchaksaraei
language : en
Publisher: BoD – Books on Demand
Release Date : 2016-12-14
Polymerase Chain Reaction For Biomedical Applications written by Ali Samadikuchaksaraei and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-12-14 with Science categories.
Do you want to know the details that should be taken into consideration in order to have accurate conventional and real-time PCR results? If so, this book is for you. Polymerase Chain Reaction for Biomedical Applications is a collection of chapters for both novice and experienced scientists and technologists aiming to address obtaining an optimized real-time PCR result, simultaneous processing of a large number of samples and assays, performing PCR and RT-PCR on cell lysate without extraction of DNA or RNA, detecting false-positive PCR results, detecting organisms in viral and microbial diseases and hospital environment, following safety assessments of food products, and using PCR for introduction of mutations. This is a must-have book for any PCR laboratory.
Pcr Protocols
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Author : Michael A. Innis
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Pcr Protocols written by Michael A. Innis and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. Avoid contamination--with specific instructions on setting up your lab Avoid cumbersome molecular biological techniques Discover new applications