Practical Immunocytochemistry In Diagnostic Cytology
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Practical Immunocytochemistry In Diagnostic Cytology
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Author : Eugenio Leonardo
language : en
Publisher: Springer Nature
Release Date : 2020-06-08
Practical Immunocytochemistry In Diagnostic Cytology written by Eugenio Leonardo and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-06-08 with Medical categories.
This book provides current information for a judicious use of conventional and molecular ICC markers performed in cytologic samples to help in the cost-effective diagnosis of superficial and deep-seated masses. By providing both an eminently practical information for appropriate technical preparation of cytologic samples and a systematic exposure of the immunophenotypic and biomolecular characteristics of normal and pathological cells, the reported morphological-molecular findings highlight the characteristics of different lesions that are useful for the practical solution of a particular differential diagnosis. Chapters include description of the cytomorphology followed by a practical ICC staining pattern for each entity. Cytologic materials include serous effusions, exfoliative cytology, fine needle aspiration cytology of superficial and deep-seated masses, and squash preparation from central nervous system masses. Practical Immunocytochemistry in Diagnostic Cytology serves as a valuable resource towards understanding critical differences between tests performed on tissue samples and those carried out on cytologic samples.
Color Atlas Of Immunocytochemistry In Diagnostic Cytology
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Author : Parvin Ganjei-Azar
language : en
Publisher: Springer
Release Date : 2006-10-13
Color Atlas Of Immunocytochemistry In Diagnostic Cytology written by Parvin Ganjei-Azar and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-10-13 with Medical categories.
This atlas examines the benefits and limitations of immunocytochemistry (ICC) in contemporary cytology. The text addresses offers practical advice on choice of markers, and illustrates the use of ICC in fine needle aspiration and body cavity fluid cytology. This practical handbook will allow for quick reference in the selection and interpretation of markers in specific differential diagnostic in the daily practice of diagnostic cytology. Includes 299 color illustrations.
Immunocytochemistry In Diagnostic Cytology
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Author : Pranab Dey
language : en
Publisher: Jaypee Brothers Medical Publishers
Release Date : 2021-01-31
Immunocytochemistry In Diagnostic Cytology written by Pranab Dey and has been published by Jaypee Brothers Medical Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-01-31 with Medical categories.
Cytology refers to a branch of pathology that deals with making diagnoses of diseases and conditions through the examination of tissue samples from the body (MedicineNet.com). Immunocytochemistry is a laboratory method that uses antibodies to check for certain antigens (markers) in a sample of cells. The antibodies are usually linked to an enzyme or a fluorescent dye. After the antibodies bind to the antigen in the cell sample, the enzyme or dye is activated, and the antigen can then be seen under a microscope. Immunocytochemistry is used to help diagnose diseases, such as cancer. It may also be used to help tell the difference between different types of cancer (National Cancer Institute). This book is a comprehensive guide to the techniques and application of immunocytochemistry in cytology. Divided into two parts, the first section discusses basic principles and preparation, techniques and quality control, and automated immune staining. Section Two covers diagnostic applications of immunocytochemistry for many different types of tumour. The text is highly illustrated with microphotographs, tables and boxes to assist learning and interpretation of findings for accurate diagnosis. Key points Comprehensive guide to techniques and application of immunocytochemistry in cytology Provides thorough understanding of basic principles and methods Covers diagnostic applications for many different types of tumour Highly illustrated with microphotographs, tables and boxes
Effusion Cytology
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Author : Parvin Ganjei-Azar, MD
language : en
Publisher: Demos Medical Publishing
Release Date : 2011-02-22
Effusion Cytology written by Parvin Ganjei-Azar, MD and has been published by Demos Medical Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-02-22 with Medical categories.
Effusion Cytology is a practical manual in diagnosis and interpretation of body cavity fluid (BCF) specimens. This highly illustrated volume will provide handy information for residents, fellows, and general pathologists with a step-by-step guide to evaluation of BCF specimens with the specific goal of identification of malignancies. Use of ancillary techniques such as immunocytochemistry is discussed where appropriate. Special attention is given to the formulation of final cytologic reports of the diagnosis of difficult cases. --Book Jacket.
New Frontiers In Cytology
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Author : Klaus Goerttler
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
New Frontiers In Cytology written by Klaus Goerttler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Diagnostic cytology has recently enjoyed increased attention and significance in modern research. Essential information on latest developments in methods and applications in cytology is provided by this book. Chapters review methodological advances, such as in cancer detection, and explore potential relationships to molecular biology. Also discussed are: viral infection, fundamentals of quantitative methods, and the revolutionary role of immunocytochemistry in diagnostic cell typing. The new insights offered by transmission and scanning electron microscopy into cellular structure and function are discussed, and the connections between cytology and histology are highlighted. Epidemiology in connection with cytology is incorporated in special reports. The current developments described here will become routine methods of the cytology of tomorrow.
Nongynecologic Cytopathology
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Author : Peter Spieler
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-07-27
Nongynecologic Cytopathology written by Peter Spieler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-07-27 with Medical categories.
This manual for diagnostic cytologists offers detailed guidance on diagnostic problems likely to be encountered in everyday practice. It encompasses exfoliative and aspiration cytology of all major nongynecologic body sites. Each chapter opens with an algorithm that presents the reader with the relevant microscopic findings, the most important additional findings, and the differential diagnostic possibilities and problems in a clear and easily remembered form. Another important feature is the wealth of high-quality color photomicrographs, which clearly document the visual appearances of the most important lesions and highlight the differential diagnostic difficulties. The accompanying text contains helpful general remarks and presents further relevant information on diagnostics, differential diagnostic procedures, and auxiliary methods. Besides established cytologists and pathologists, cytopathologists in training and cytotechnologists will find this book to be a valuable aid.
Practical Diagnostic Approaches In Non Gynaecologic Cytology
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Author : Min En Nga
language : en
Publisher: Springer Nature
Release Date : 2020-07-31
Practical Diagnostic Approaches In Non Gynaecologic Cytology written by Min En Nga and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-31 with Medical categories.
This book presents rational diagnostic approaches to common areas of cytopathology, such as thyroid, lymph node and effusion cytology. It discusses differential diagnoses for each site, and describes a systematic approach to narrow down differential diagnoses in a logical manner based on cytomorphology and the judicious application of ancillary tests. Further, it introduces discriminatory panels of immunohistochemical tests, with an emphasis on patient-centred approaches with active clinicopathologic correlations. In addition, it provides practical recommendations for optimizing tissue triage for ancillary testing, in terms of both diagnostic and therapy-related testing. The book also includes sample diagnostic reports to help readers formulate appropriate comments and to aid clinicians in specific clinical scenarios, as well as test cases for readers to apply their diagnostic and specimen triage algorithms. The book equips readers to apply logical approaches to sound cytopathology reporting in daily clinical practice, guiding them through specimen collection and triage to diagnostic workup based on morphologic and clinical features, and writing rational and clinically useful diagnostic cytology reports with a focus on clinicopathologic correlation. As such it is relevant for practising cytopathologists and pathology trainees as well as for cytologists (cytotechnologists) and other clinicians involved in cytopathology diagnostic processes.
Practical Cytopathology
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Author : Huihong Xu
language : en
Publisher: Springer
Release Date : 2019-11-18
Practical Cytopathology written by Huihong Xu and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-11-18 with Medical categories.
This book provides a comprehensive, practical, and state-of-the art review addressing the major issues and challenges in cytopathology practice using a question and answer format. Making an accurate diagnosis, especially on a limited cytology sample obtained by minimally invasive procedures, is often challenging, yet crucial to patient care. Using the most current and evidence-based approaches, this book: 1) focuses on frequently asked questions in day-to-day practice of cytopathology as well as surgical pathology; 2) provides quick, accurate, and useful answers; 3) emphasizes the importance of clinical, radiological, and cytological correlation, as well as cyto-histological correlation; and 4) delineates how to judiciously use immunohistochemistry, molecular tests, flow cytometry, cytogenetics, and other established ancillary studies including next generation sequencing and computer-assisted diagnostics. Chapters are written by experts in their fields and provide the most up-to-date information in the field of cytopathology. Practical Cytopathology: Frequently Asked Questions serves as a practical resource and guide to relevant references for trainees, cytotechnologists, and cytopathologists at various skill levels.
Immunocytochemistry
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Author : Richard W. Burry
language : en
Publisher: Springer
Release Date : 2009-12-17
Immunocytochemistry written by Richard W. Burry and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-12-17 with Science categories.
Description: In biomedical research, because of a dramatic increase in productivity, immunocytochemistry has emerged as a major technique. The proposed book will provide the first practical guide to planning, performing, and evaluating immunocytochemical experiments. In today’s graduate education the emphasis is on doing research and not on formal class work. Graduate students therefore lack the background in many essential techniques necessary to perform research in fields in which they were not trained. As director of a university core microscopy facility which sees students and faculty from dozens of laboratories each year, Dr. Burry has surmised the vast majority of these novice microscope users need considerable help. In an attempt to educate users, Dr. Burry has initiated immunocytochemistry seminars and workshops which serve to train people in this powerful research tool. The proposed book is an outgrowth of these presentations and conversations with, by now, hundreds of people who have asked for help. The philosophy which separates this book from other books in this field is that it is practical, rather than academic. In looking at other important immunocytochemistry titles, the predominant orientation is academic, with the author attempting to comprehensively discuss the topic. For example, one book with sample preparation lists ten fixatives which can be used; however, only two such fixatives are commonly used today. In this particular title, the detailed discussion of old methods might be seen as important in establishing the author as an expert. By contrast, the approach for Burry’s book would be to discuss methods based on what works in animal research laboratories today, and focus only on the most productive methods. An additional distinction with this proposed book is the focus on animal research and not human pathology. There is a certification program for pathology technicians which requires them to learn a set body of material based on processing human tissue for examination by a pathologist. Many of the books on immunocytochemistry aim at this large pathology user base. Due to historical reasons, pathology laboratories process human tissues in a specific way and embed the tissue in paraffin, as has been done for over a century. In the last ten years, the power of immunocytochemistry in clinical diagnosis has become clear and has accordingly been adapted to pathology. However, the extensive processing needed for paraffin sections is not needed if the tissues are from research animals. Processing for animal-based tissues takes about a third of the time and results in higher quality images. The focus of this book is on processing these animal research tissues for immunocytochemistry. Today, there are no technique books which are aimed at this user base. As a subject matter expert in the area of the proposed book, Dr. Burry will make recommendations and offer opinions. Because this field is new and is emerging, there are numerous advantages of specific methods over other, more generalized methods. The purpose of this book is to show a novice how to do immunocytochemistry without engaging in a discussion of possible advanced methods. For the advanced user, there are several good books which discuss the unusual methods, yet for the novice there are currently none. Main Author : Richard W. Burry, The Ohio State University (United States). The Outline of the Book : Each chapter supplies a set of important principals and steps necessary for good immunocytochemistry. The information is distilled down to include only the most important points and does not attempt to cover infrequently used procedures or reagents. At the end of most chapters is a section on trouble-shooting many of the common problems using the Sherlock Holmes method. Each chapter also includes specific protocols which can be used. The goal of each chapter is to present the reader with enough information to successfully design experiments and solve many of the problems one may encounter. Using immunocytochemical protocols without the understanding of their workings is not advised, as the user will need to evaluate his or her results to determine whether the results are reliable. Such evaluation is extremely important for users who need reliable images which will clearly answer important scientific questions. 1. Introduction Definitions (immunocytochemistry and immunohistochemistry) Scope: animal research and not human pathology, paraffin sections, epitope retrieval, or immunohistochemistry Focus: fluorescence and enzyme detection Why do immunocytochemistry? Immunocytochemistry "individual study" rather than "population study" Example of a two-label experiment What is included in these chapters? Overview of the theory Background with enough information to help solve common problems. Advantages and disadvantages of different options Opinions and suggestions 2. Fixation and Sectioning Chemistry of fixation Denaturing vs cross-linking fixatives Application of fixative Perfusion, drop-in, cultures, fresh-frozen Selection of sample section type Sectioning tissue Rapid freezing, cryostat, freezing microtome, vibratome Storage of tissue Protocols 3. Antibodies Introduction Isoforms, structure, reactivity Generation Polyclonal vs monoclonal Antibodies as reagents Antibody specificity and sources Storage and handling 4. Labels for antibodies Fluorescence, enzymes and particulates Fluorescence theory Fluorescent labels - four generations Enzymes theory Selecting enzymes vs. fluorescence Selecting a label- advantages and disadvantages Protocols 5. Methods of applying antibodies Direct method Indirect method Antibody amplification methods ABC TSA Protocols 6. Blocking and Permeability Theory of blocking Theory of detergents Protocols 7. Procedure- Single primary antibody Planning steps Sample, fixation, sectioning Vehicle Antibody dilutions Controls Protocols 8. Multiple primary antibodies - primary antibodies of different species Procedure Controls Protocols 9. Multiple primary antibodies-primary antibodies of same species Block-between Zenon HRP-chromogen development High-titer incubations Controls Protocols 10. Microscopy Wide-field fluorescence microscope Confocal microscope Bright field—enzyme chromogen Choice Problems 11. Images Size, intensity, and pixels Manipulation—what is ethical? Manuscript Figures 11. Planning and Troubleshooting Scheme for discussion-making in planning experiments Case studies with Sherlock Holmes detective work 12. So you want to do electron microscopic ICC? Criteria in decision-making Summary of the two techniques
Practical Cytopathology
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Author : Huihong Xu
language : en
Publisher:
Release Date : 2020
Practical Cytopathology written by Huihong Xu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020 with Cytodiagnosis categories.
This book provides a comprehensive, practical, and state-of-the art review addressing the major issues and challenges in cytopathology practice using a question and answer format. Making an accurate diagnosis, especially on a limited cytology sample obtained by minimally invasive procedures, is often challenging, yet crucial to patient care. Using the most current and evidence-based approaches, this book: 1) focuses on frequently asked questions in day-to-day practice of cytopathology as well as surgical pathology; 2) provides quick, accurate, and useful answers; 3) emphasizes the importance of clinical, radiological, and cytological correlation, as well as cyto-histological correlation; and 4) delineates how to judiciously use immunohistochemistry, molecular tests, flow cytometry, cytogenetics, and other established ancillary studies including next generation sequencing and computer-assisted diagnostics. Chapters are written by experts in their fields and provide the most up-to-date information in the field of cytopathology. Practical Cytopathology: Frequently Asked Questions serves as a practical resource and guide to relevant references for trainees, cytotechnologists, and cytopathologists at various skill levels.