Practical Methods In Electron Microscopy A W Robards And U B Sleytr Low Temperature Methods In Biological Electron Microscopy

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Practical Methods In Electron Microscopy A W Robards And U B Sleytr Low Temperature Methods In Biological Electron Microscopy

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Author : Audrey M. Glauert
language : en
Publisher:
Release Date : 1972

Practical Methods In Electron Microscopy A W Robards And U B Sleytr Low Temperature Methods In Biological Electron Microscopy written by Audrey M. Glauert and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1972 with Electron microscopes categories.

Electron Microscopy Methods And Protocols

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Author : M. A. Nasser Hajibagheri
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02

Electron Microscopy Methods And Protocols written by M. A. Nasser Hajibagheri and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.

Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).

Methods Of Preparation For Electron Microscopy

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Author : David G. Robinson
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Methods Of Preparation For Electron Microscopy written by David G. Robinson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the reso lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus.

Cryotechniques In Biological Electron Microscopy

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Author : Rudolf A. Steinbrecht
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Cryotechniques In Biological Electron Microscopy written by Rudolf A. Steinbrecht and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.

Low Temperature Microscopy And Analysis

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Author : Patrick Echlin
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11

Low Temperature Microscopy And Analysis written by Patrick Echlin and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.

The frozen-hydrated specimen is the principal element that unifies the subject of low temperature microscopy, and frozen-hydrated specimens are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure and composition to investigate. Yet all share a body of com mon fact and theory upon which their work must be based. Low-Temperature Micros copy and Analysis provides, for the first time, a comprehensive treatment of all the elements to which one needs access. What is the appeal behind the use of frozen-hydrated specimens for biological electron microscopy, and why is it so important that such a book should now have been written? If one cannot observe dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all the more necessary in electron microscopy because of the enormous increase in resolution that is available with electron-optical instruments, compared to light optical microscopes.

Biological Field Emission Scanning Electron Microscopy

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Author : Roland A. Fleck
language : en
Publisher: John Wiley & Sons
Release Date : 2019-02-06

Biological Field Emission Scanning Electron Microscopy written by Roland A. Fleck and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-02-06 with Science categories.

The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.

Electron Microscopy Of Subcellular Dynamics

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Author : Helmut Plattner
language : en
Publisher: CRC Press
Release Date : 1989-09-30

Electron Microscopy Of Subcellular Dynamics written by Helmut Plattner and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989-09-30 with Science categories.

This illustrated volume surveys the correlated use of currently available methods of electron microscopic techniques, along with the goals and perspectives for future developments. The authors discuss an integrative approach of different EM preparation and analysis techniques that can allow for an analysis of dynamic cellular processes with high temporal and spatial resolution on the electron microscope level. This concise, yet thorough, work is a valuable reference for researchers in the field.

Biological Specimen Preparation For Transmission Electron Microscopy

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Author : Audrey M. Glauert
language : en
Publisher: Princeton University Press
Release Date : 2014-07-14

Biological Specimen Preparation For Transmission Electron Microscopy written by Audrey M. Glauert and has been published by Princeton University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-07-14 with Science categories.

This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements. All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs. Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor Originally published in 1999. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.

Gray S Anatomy E Book

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Author :
language : en
Publisher: Elsevier Health Sciences
Release Date : 2015-09-25

Gray S Anatomy E Book written by and has been published by Elsevier Health Sciences this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-09-25 with Medical categories.

In 1858, Drs. Henry Gray and Henry Vandyke Carter created a book for their surgical colleagues that established an enduring standard among anatomical texts. After more than 150 years of continuous publication, Gray's Anatomy remains the definitive, comprehensive reference on the subject, offering ready access to the information you need to ensure safe, effective practice. This 41st edition has been meticulously revised and updated throughout, reflecting the very latest understanding of clinical anatomy from field leaders around the world. The book's traditional lavish art programme and clear text have been further honed and enhanced, while major advances in imaging techniques and the new insights they bring are fully captured in new state-of-the-art X-ray, CT, MR, and ultrasonic images. - Presents the most detailed and dependable coverage of anatomy available anywhere. - Regional organization collects all relevant material on each body area together in one place, making access to core information easier for clinical readers. - Anatomical information is matched with key clinical information where relevant. - Numerous clinical discussions emphasize considerations that may affect medical care. - Each chapter has been edited by experts in their field, ensuring access to the very latest evidence-based information on that topic. - More than 1,000 completely new photographs, including an extensive electronic collection of the latest X-ray, CT, MR, and histological images. - The downloadable Expert Consult eBook version included with your purchase allows you to search all of the text, figures, references and videos from the book on a variety of devices. - Carefully selected electronic enhancements include additional text, tables, illustrations, labelled imaging and videos – as well as 24 specially invited 'Commentaries' on new and emerging topics related to anatomy.

Protocols In Lichenology

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Author : Ilse Kranner
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Protocols In Lichenology written by Ilse Kranner and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

As an intricate association between a fungus and one or more green algae or cyanobacteria, lichens are one of the most successful examples of symbiosis. These fascinating organisms survive extreme desiccation and temperatures. They are adapted to a great variety of habitats, from deserts to intertidal zones, from tropical rain forests to the peaks of the Himalayas and to circumpolar ecosystems. Lichens are extremely efficient accumulators of atmospherically deposited pollutants, and are therefore widely used to monitor environmental pollution. Their wide range of secondary products show pharmaceutically interesting fungicidal, antibacterial and antiviral properties. Lichens are extremely difficult to culture. This manual provides well-tested tissue culture protocols, protocols for studying lichen ultrastructure, (eco)physiology, primary and secondary compounds, and for using lichens as bioindicators.