Practical Methods In Electron Microscopy Pt 1 Fixation Dehydration And Embedding Of Biological Specimens
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Methods Of Preparation For Electron Microscopy
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Author : David G. Robinson
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Methods Of Preparation For Electron Microscopy written by David G. Robinson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the reso lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus.
Practical Methods In Electron Microscopy Pt 1 Fixation Dehydration And Embedding Of Biological Specimens By A M Glauert
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Author : Audry M. Glauert
language : en
Publisher:
Release Date : 1972
Practical Methods In Electron Microscopy Pt 1 Fixation Dehydration And Embedding Of Biological Specimens By A M Glauert written by Audry M. Glauert and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1972 with Electron microscopy categories.
Practical Methods In Electron Microscopy Pt 1 Williams M A Autoradiography And Immunocytochemistry
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Author : Audry M. Glauert
language : en
Publisher:
Release Date : 1977
Practical Methods In Electron Microscopy Pt 1 Williams M A Autoradiography And Immunocytochemistry written by Audry M. Glauert and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1977 with Electron microscopy categories.
Practical Methods In Electron Microscopy Pt 1 Glauert A M Fixation Dehydration And Embedding Of Biological Specimens
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Author : Audry M. Glauert
language : en
Publisher:
Release Date : 1972
Practical Methods In Electron Microscopy Pt 1 Glauert A M Fixation Dehydration And Embedding Of Biological Specimens written by Audry M. Glauert and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1972 with Electron microscopy categories.
Practical Methods In Electron Microscopy Pt 1 Fixation Dehydration And Embedding Of Biological Specimens
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Author : Audrey M. Glauert
language : en
Publisher:
Release Date : 1972
Practical Methods In Electron Microscopy Pt 1 Fixation Dehydration And Embedding Of Biological Specimens written by Audrey M. Glauert and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1972 with Electron microscopy categories.
Practical Methods In Electron Microscopy Pt 1 Lewis P R Staining Methods For Sectioned Material
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Author : Audrey M. Glauert
language : en
Publisher:
Release Date : 1977
Practical Methods In Electron Microscopy Pt 1 Lewis P R Staining Methods For Sectioned Material written by Audrey M. Glauert and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1977 with Electron microscopy categories.
Practical Methods In Electron Microscopy Pt 1 Lewis P R Staining Methods For Sectioned Material
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Author : Audry M. Glauert
language : en
Publisher:
Release Date : 1972
Practical Methods In Electron Microscopy Pt 1 Lewis P R Staining Methods For Sectioned Material written by Audry M. Glauert and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1972 with Electron microscopy categories.
Practical Methods In Electron Microscopy Pt 1 Staining Methods For Sectioned Material
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Author : Audrey M. Glauert
language : en
Publisher:
Release Date : 1977
Practical Methods In Electron Microscopy Pt 1 Staining Methods For Sectioned Material written by Audrey M. Glauert and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1977 with Electron microscopy categories.
Biological Specimen Preparation For Transmission Electron Microscopy
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Author : Audrey M. Glauert
language : en
Publisher: Princeton University Press
Release Date : 2014-07-14
Biological Specimen Preparation For Transmission Electron Microscopy written by Audrey M. Glauert and has been published by Princeton University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-07-14 with Science categories.
This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements. All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs. Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor Originally published in 1999. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.
National Library Of Medicine Current Catalog
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Author : National Library of Medicine (U.S.)
language : en
Publisher:
Release Date : 1971
National Library Of Medicine Current Catalog written by National Library of Medicine (U.S.) and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1971 with Medicine categories.