Practical Techniques In Molecular Biotechnology
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Practical Techniques In Molecular Biotechnology
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Author : Bal Ram Singh
language : en
Publisher: Cambridge University Press
Release Date : 2022-06-16
Practical Techniques In Molecular Biotechnology written by Bal Ram Singh and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-06-16 with Medical categories.
The book will be useful for undergraduate students as a supplementary/reference text in the field of molecular biotechnology.
Advanced Methods In Molecular Biology And Biotechnology
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Author : Khalid Z. Masoodi
language : en
Publisher: Academic Press
Release Date : 2020-10-28
Advanced Methods In Molecular Biology And Biotechnology written by Khalid Z. Masoodi and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-28 with Science categories.
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Basic Techniques In Molecular Biology
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Author : Stefan Surzycki
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Basic Techniques In Molecular Biology written by Stefan Surzycki and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Molecular Biology Techniques
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Author : Heather B. Miller
language : en
Publisher: Academic Press
Release Date : 2011-10-18
Molecular Biology Techniques written by Heather B. Miller and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-18 with Science categories.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Principles And Techniques Of Biochemistry And Molecular Biology
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Author : Keith Wilson
language : en
Publisher: Cambridge University Press
Release Date : 2010-03-04
Principles And Techniques Of Biochemistry And Molecular Biology written by Keith Wilson and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-03-04 with Medical categories.
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Techniques In Molecular Biology
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Author : J.M. Walker
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Techniques In Molecular Biology written by J.M. Walker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.
Wilson And Walker S Principles And Techniques Of Biochemistry And Molecular Biology
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Author : Keith Wilson
language : en
Publisher: Cambridge University Press
Release Date : 2018-04-19
Wilson And Walker S Principles And Techniques Of Biochemistry And Molecular Biology written by Keith Wilson and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-19 with Medical categories.
A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research.
Experiments In Molecular Biology
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Author : Robert J. Slater
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-07
Experiments In Molecular Biology written by Robert J. Slater and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-07 with Science categories.
Research in the field of molecular biology has progressed at a fascinating rate in recent years. Much of this progress results from the development of new laboratory techniques that allow very precise fractionation and analysis of nucleic acids and proteins, as well as the construction of recom binant DNA molecules that can then be cloned and expressed in host cells. Progress has been so rapid that there has been a shortfall in the training of appropriately qualified staff. Many existing laboratory workers require retraining, and many educational institutions have had difficulty incor porating the new molecular biology techniques into their teaching programs. Although there are several manuals currently available that describe laboratory techniques in molecular biology, they are principally written for the indivi dual research worker and are not intended for use in the design of practical classes for students. The aim of this book is to provide just such a series of pro tocols forthe teaching of practical molecular biology. The idea arose following the success of several Workshops in Molecular Biology, organized and taught by staff in the Biol ogy Department of the Hatfield Polytechnic. Gradually, the protocols used in the workshops have been incorporated into the Hatfield undergraduate and postgraduate teaching pro grams and have now been collected together to form a book.
Techniques In Molecular Medicine
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Author : Friedhelm Hildebrandt
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Techniques In Molecular Medicine written by Friedhelm Hildebrandt and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Medical categories.
Standard molecular biology techniques are used widely in biomedical re search and have had a major impact on the understanding of physiologic processes and disease mechanisms. They also play an increasing role in mo lecular genetic diagnosis and new therapeutic approaches. This laboratory manual is designed for researchers who need reliable pro tocols for their laboratorywork. It provides a step by step approach to stan dard methods of molecular biology. A wide range of topics is covered by protocols for immediate application in the laboratory. These include pur ification, isolation, labeling, hybridization, sequencing and enzymatic mod ification ofDNA or RNA, polymerase chain reaction, cloning, libraries, and protein techniques. The manual is based on experience with a practical course in molecular biologytechniques, which was taught bythe authors. Since most methods in molecular biology recapitulate in a test tube mechanisms that occur natu rally in the cell, the first three chapters describe and illustrate basic mechan isms of molecular biology. U nderstanding more about these basic mechan isms will enable the researcher to apply techniques more specifically, based on the knowledge of their potential and limits. W e hope that users find this manual a helpful guide on their road to exciting insights.
A Practical Guide To Protein Engineering
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Author : Tuck Seng Wong
language : en
Publisher: Springer Nature
Release Date : 2020-10-29
A Practical Guide To Protein Engineering written by Tuck Seng Wong and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-29 with Science categories.
This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.